Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,637	T→C	intergenic (+50063/+5528)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,637	0	T	C	100.0%	106.2 / NA	38	intergenic (+50063/+5528)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/22);  total (16/22)

ATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000399‑1000752                                                                                                                                                                                                                                               |                                                                                                                    aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                                         >  1:10290/1‑244 (MQ=255) aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                                         >  1:840595/1‑244 (MQ=255)      gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    >  1:188738/5‑251 (MQ=255)      gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    >  1:669865/5‑251 (MQ=255)       acctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTAGATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAAt                                                                                                   <  1:450901/248‑1 (MQ=255)              cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                                            >  1:228148/1‑251 (MQ=255)              cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                                            >  1:277073/1‑251 (MQ=255)                caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                                          >  1:421895/1‑251 (MQ=255)                caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                                          >  1:410129/1‑251 (MQ=255)                       aggTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAg                                                                                    <  1:857298/248‑1 (MQ=255)                            aTGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATg                                                                                                  <  1:552000/231‑1 (MQ=255)                                            aGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtata                                                              >  1:665540/1‑245 (MQ=255)                                              gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtatat                                                             <  1:623153/250‑8 (MQ=255)                                                aTTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:491142/251‑1 (MQ=255)                                                  tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:400564/251‑1 (MQ=255)                                                  tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:744739/251‑1 (MQ=255)                                                         aTGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACg                                                  >  1:657619/1‑250 (MQ=255)                                                                 ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:39444/226‑1 (MQ=255)                                                                  gattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTG‑CGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCa                                       >  1:193324/8‑251 (MQ=255)                                                                      acgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           <  1:592328/225‑1 (MQ=255)                                                                                aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:629036/251‑1 (MQ=255)                                                                                aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:93247/251‑1 (MQ=255)                                                                                  ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:425820/1‑251 (MQ=255)                                                                                  ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:87745/1‑251 (MQ=255)                                                                                   gTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGt                                                                                   <  1:518623/191‑1 (MQ=255)                                                                                       gAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATg                                                         <  1:792671/213‑1 (MQ=255)                                                                                        aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:604529/250‑4 (MQ=255)                                                                                        aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:545586/250‑4 (MQ=255)                                                                                                                     gcggAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           >  1:269537/4‑181 (MQ=255)                                                                                                                        gAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtatgttattt  <  1:818686/235‑21 (MQ=255)                                                                                                                             tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      >  1:488859/1‑159 (MQ=255)                                                                                                                             tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaa                  <  1:225443/214‑5 (MQ=255)                                                                                                                               tttACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAATCTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:79663/211‑4 (MQ=255)                                                                                                                                     cacaAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      <  1:767337/154‑4 (MQ=255)                                                                                                                                         aaGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:491282/194‑1 (MQ=255)                                                                                                                                                 gtAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    <  1:144322/111‑1 (MQ=255)                                                                                                                                                                                 aTAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtgtgt       >  1:448188/1‑158 (MQ=255)                                                                                                                                                                                  tAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAgg                                  <  1:43191/145‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                    ATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000399‑1000752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑