Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,622	C→T	intergenic (+50048/+5543)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,622	0	C	T	100.0%	119.3 / NA	43	intergenic (+50048/+5543)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/25);  total (18/25)

TACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000376‑1000752                                                                                                                                                                                                                                                       |                                                                                                                                   tACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACg                                                                                                                                <  1:508055/251‑1 (MQ=255)   cAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACggg                                                                                                                              <  1:826244/251‑1 (MQ=255)            ttgaaTGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGagatttcac                                                                                                                     <  1:50896/248‑9 (MQ=255)                        aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                                         >  1:10290/1‑244 (MQ=255)                        aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                                         >  1:840595/1‑244 (MQ=255)                             gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    >  1:669865/5‑251 (MQ=255)                             gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    >  1:188738/5‑251 (MQ=255)                              acctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTAGATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAAt                                                                                                   <  1:450901/248‑1 (MQ=255)                                     cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGaga                                                                                                                           >  1:467730/1‑218 (MQ=255)                                     cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                                            >  1:228148/1‑251 (MQ=255)                                     cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                                            >  1:277073/1‑251 (MQ=255)                                       caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                                          >  1:410129/1‑251 (MQ=255)                                       caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                                          >  1:421895/1‑251 (MQ=255)                                              aggTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAg                                                                                    <  1:857298/248‑1 (MQ=255)                                                   aTGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATg                                                                                                  <  1:552000/231‑1 (MQ=255)                                                                   aGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtata                                                              >  1:665540/1‑245 (MQ=255)                                                                     gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtatat                                                             <  1:623153/250‑8 (MQ=255)                                                                       aTTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:491142/251‑1 (MQ=255)                                                                         tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:744739/251‑1 (MQ=255)                                                                         tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:400564/251‑1 (MQ=255)                                                                                aTGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACg                                                  >  1:657619/1‑250 (MQ=255)                                                                                        ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGaga                                                                                                                           >  1:176733/9‑167 (MQ=255)                                                                                        ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:39444/226‑1 (MQ=255)                                                                                         gattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTG‑CGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCa                                       >  1:193324/8‑251 (MQ=255)                                                                                             acgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           <  1:592328/225‑1 (MQ=255)                                                                                                       aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:93247/251‑1 (MQ=255)                                                                                                       aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:629036/251‑1 (MQ=255)                                                                                                         ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:425820/1‑251 (MQ=255)                                                                                                         ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:87745/1‑251 (MQ=255)                                                                                                          gTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGt                                                                                   <  1:518623/191‑1 (MQ=255)                                                                                                              gAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATg                                                         <  1:792671/213‑1 (MQ=255)                                                                                                               aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:604529/250‑4 (MQ=255)                                                                                                               aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:545586/250‑4 (MQ=255)                                                                                                                                            gcggAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           >  1:269537/4‑181 (MQ=255)                                                                                                                                               gAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtatgttattt  <  1:818686/235‑21 (MQ=255)                                                                                                                                                    tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      >  1:488859/1‑159 (MQ=255)                                                                                                                                                    tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaa                  <  1:225443/214‑5 (MQ=255)                                                                                                                                                      tttACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAATCTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:79663/211‑4 (MQ=255)                                                                                                                                                            cacaAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      <  1:767337/154‑4 (MQ=255)                                                                                                                                                                aaGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:491282/194‑1 (MQ=255)                                                                                                                                                                        gtAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    <  1:144322/111‑1 (MQ=255)                                                                                                                                                                                                        aTAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtgtgt       >  1:448188/1‑158 (MQ=255)                                                                                                                                                                                                         tAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAgg                                  <  1:43191/145‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                   TACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000376‑1000752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑