Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,553	A→G	intergenic (+49979/+5612)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,553	0	A	G	100.0%	212.7 / NA	73	intergenic (+49979/+5612)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/37);  total (36/37)

AACTCATCTTCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000311‑1000752                                                                                                                                                                                                                                                   |                                                                                                                                                                                                        aactcactttCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACaa                                                                                                                                                                                                 <  1:847551/243‑1 (MQ=255)           cAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                                                                                                                                                                             >  1:547591/1‑245 (MQ=255)                  gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                >  1:690848/1‑251 (MQ=255)                  gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                >  1:776313/1‑251 (MQ=255)                  gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                >  1:848303/1‑251 (MQ=255)                   agtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                <  1:345053/246‑1 (MQ=255)                    gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGa                                                                                                                                                                                        <  1:574803/238‑1 (MQ=255)                    gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                                              >  1:686141/4‑251 (MQ=255)                    gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                                              >  1:380722/4‑251 (MQ=255)                     tattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                                              <  1:87181/248‑1 (MQ=255)                             gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTc                                                                                                                                                                          >  1:24566/2‑246 (MQ=255)                             gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCa                                                                                                                                                                     >  1:267620/2‑251 (MQ=255)                             gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCa                                                                                                                                                                     >  1:282192/2‑251 (MQ=255)                                        gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                                                                                                                                                                             >  1:615472/2‑216 (MQ=255)                                        gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                >  1:166077/2‑229 (MQ=255)                                        gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                >  1:142115/2‑229 (MQ=255)                                          ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                                                <  1:560529/227‑1 (MQ=255)                                          ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGtt                                                                                                                                                        >  1:839028/1‑251 (MQ=255)                                                ctgaagaGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                                                                                                                                                                             <  1:417789/206‑1 (MQ=255)                                                             cTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAc                                                                                                                                     <  1:790983/251‑1 (MQ=255)                                                             cTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAc                                                                                                                                     <  1:883757/251‑1 (MQ=255)                                                             cTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCACAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAc                                                                                                                                     <  1:504438/251‑1 (MQ=255)                                                              tttttACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAct                                                                                                                                    <  1:2064/251‑2 (MQ=255)                                                               ttttACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCtata                                                                                                                                      >  1:265917/1‑248 (MQ=255)                                                                  tACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACg                                                                                                                                <  1:508055/251‑1 (MQ=255)                                                                    cAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACggg                                                                                                                              <  1:826244/251‑1 (MQ=255)                                                                       ggTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                                              <  1:814858/200‑1 (MQ=255)                                                                          gtCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATActg                                                                                                                                   >  1:109825/1‑238 (MQ=255)                                                                             ttgaaTGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGagatttcac                                                                                                                     <  1:50896/248‑9 (MQ=255)                                                                                         aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                                         >  1:10290/1‑244 (MQ=255)                                                                                         aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                                         >  1:840595/1‑244 (MQ=255)                                                                                              gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    >  1:669865/5‑251 (MQ=255)                                                                                              gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    >  1:188738/5‑251 (MQ=255)                                                                                               acctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTAGATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAAt                                                                                                   <  1:450901/248‑1 (MQ=255)                                                                                                    cTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCAt                                                                                                                                                                                           <  1:135188/158‑1 (MQ=255)                                                                                                      cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                                              >  1:430895/1‑169 (MQ=255)                                                                                                      cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGaga                                                                                                                           >  1:467730/1‑218 (MQ=255)                                                                                                      cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                                            >  1:228148/1‑251 (MQ=255)                                                                                                      cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                                            >  1:277073/1‑251 (MQ=255)                                                                                                        caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGcc                                                                                                                                          >  1:821727/1‑203 (MQ=255)                                                                                                        caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                                          >  1:421895/1‑251 (MQ=255)                                                                                                        caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                                          >  1:410129/1‑251 (MQ=255)                                                                                                               aggTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAg                                                                                    <  1:857298/248‑1 (MQ=255)                                                                                                                    aTGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATg                                                                                                  <  1:552000/231‑1 (MQ=255)                                                                                                                                    aGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtata                                                              >  1:665540/1‑245 (MQ=255)                                                                                                                                      gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATActg                                                                                                                                   <  1:144242/180‑3 (MQ=255)                                                                                                                                      gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtatat                                                             <  1:623153/250‑8 (MQ=255)                                                                                                                                        aTTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:491142/251‑1 (MQ=255)                                                                                                                                         ttGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTtacat                                                                                                                                                    >  1:859494/1‑157 (MQ=255)                                                                                                                                          tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:400564/251‑1 (MQ=255)                                                                                                                                          tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:744739/251‑1 (MQ=255)                                                                                                                                                 aTGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACg                                                  >  1:657619/1‑250 (MQ=255)                                                                                                                                                         ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGAc                                                                                                                                                                                                   >  1:412830/9‑97 (MQ=255)                                                                                                                                                         ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGaga                                                                                                                           >  1:176733/9‑167 (MQ=255)                                                                                                                                                         ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:39444/226‑1 (MQ=255)                                                                                                                                                          gattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTG‑CGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCa                                       >  1:193324/8‑251 (MQ=255)                                                                                                                                                              acgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           <  1:592328/225‑1 (MQ=255)                                                                                                                                                                        aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:93247/251‑1 (MQ=255)                                                                                                                                                                        aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:629036/251‑1 (MQ=255)                                                                                                                                                                          ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:425820/1‑251 (MQ=255)                                                                                                                                                                          ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:87745/1‑251 (MQ=255)                                                                                                                                                                           gTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGt                                                                                   <  1:518623/191‑1 (MQ=255)                                                                                                                                                                               gAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATg                                                         <  1:792671/213‑1 (MQ=255)                                                                                                                                                                                aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:604529/250‑4 (MQ=255)                                                                                                                                                                                aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:545586/250‑4 (MQ=255)                                                                                                                                                                                                             gcggAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           >  1:269537/4‑181 (MQ=255)                                                                                                                                                                                                                gAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtatgttattt  <  1:818686/235‑21 (MQ=255)                                                                                                                                                                                                                     tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      >  1:488859/1‑159 (MQ=255)                                                                                                                                                                                                                     tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaa                  <  1:225443/214‑5 (MQ=255)                                                                                                                                                                                                                       tttACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAATCTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:79663/211‑4 (MQ=255)                                                                                                                                                                                                                             cacaAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      <  1:767337/154‑4 (MQ=255)                                                                                                                                                                                                                                 aaGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:491282/194‑1 (MQ=255)                                                                                                                                                                                                                                         gtAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                                    <  1:144322/111‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                        AACTCATCTTCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000311‑1000752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑