Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,517	A→G	intergenic (+49943/+5648)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,517	0	A	G	100.0%	209.7 / NA	76	intergenic (+49943/+5648)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/38);  total (38/38)

GGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCTTCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAG  >  1/1000270‑1000735                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                           ggAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGaa                                                                                                                                                                                                                         >  1:533506/1‑251 (MQ=255) ggAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGaa                                                                                                                                                                                                                         >  1:481236/1‑251 (MQ=255)       aaTATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                                                                                                                                                                                   <  1:312984/251‑1 (MQ=255)       aaTATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                                                                                                                                                                                   <  1:451611/251‑1 (MQ=255)          atGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                                                                                                                                                                                   <  1:667739/248‑1 (MQ=255)          atGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTa                                                                                                                                                                                                                 >  1:482085/1‑250 (MQ=255)                              aaaaaaTGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGa                                                                                                                                                                                            <  1:664728/251‑1 (MQ=255)                                          aactcactttCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACaa                                                                                                                                                                                <  1:847551/243‑1 (MQ=255)                                                    cAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                                                                                                                                                            >  1:547591/1‑245 (MQ=255)                                                           gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               >  1:848303/1‑251 (MQ=255)                                                           gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               >  1:690848/1‑251 (MQ=255)                                                           gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               >  1:776313/1‑251 (MQ=255)                                                            agtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               <  1:345053/246‑1 (MQ=255)                                                             gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCaaagta                                                                                                                                                                                                <  1:565035/213‑4 (MQ=255)                                                             gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGa                                                                                                                                                                       <  1:574803/238‑1 (MQ=255)                                                             gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                             >  1:686141/4‑251 (MQ=255)                                                             gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                             >  1:380722/4‑251 (MQ=255)                                                              tattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                             <  1:87181/248‑1 (MQ=255)                                                                      gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTc                                                                                                                                                         >  1:24566/2‑246 (MQ=255)                                                                      gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCa                                                                                                                                                    >  1:267620/2‑251 (MQ=255)                                                                      gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCa                                                                                                                                                    >  1:282192/2‑251 (MQ=255)                                                                                 gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                                                                                                                                                            >  1:615472/2‑216 (MQ=255)                                                                                 gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               >  1:142115/2‑229 (MQ=255)                                                                                 gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               >  1:166077/2‑229 (MQ=255)                                                                                   ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                                                                                                                                                               <  1:560529/227‑1 (MQ=255)                                                                                   ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGtt                                                                                                                                       >  1:839028/1‑251 (MQ=255)                                                                                      tAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTacacac                                                                                                                                                                                                            >  1:7649/1‑179 (MQ=255)                                                                                         ctgaagaGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                                                                                                                                                            <  1:417789/206‑1 (MQ=255)                                                                                                      cTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAc                                                                                                                    <  1:883757/251‑1 (MQ=255)                                                                                                      cTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAc                                                                                                                    <  1:790983/251‑1 (MQ=255)                                                                                                      cTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCACAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAc                                                                                                                    <  1:504438/251‑1 (MQ=255)                                                                                                       tttttACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATAct                                                                                                                   <  1:2064/251‑2 (MQ=255)                                                                                                        ttttACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCtata                                                                                                                     >  1:265917/1‑248 (MQ=255)                                                                                                           tACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACg                                                                                                               <  1:508055/251‑1 (MQ=255)                                                                                                             cAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACggg                                                                                                             <  1:826244/251‑1 (MQ=255)                                                                                                                ggTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                             <  1:814858/200‑1 (MQ=255)                                                                                                                   gtCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATActg                                                                                                                  >  1:109825/1‑238 (MQ=255)                                                                                                                      ttgaaTGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGagatttcac                                                                                                    <  1:50896/248‑9 (MQ=255)                                                                                                                                 aaTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAg                                                                                                                                                                                          <  1:546373/154‑1 (MQ=255)                                                                                                                                  aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                        >  1:10290/1‑244 (MQ=255)                                                                                                                                  aTTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACAtttttccactt                                                                                        >  1:840595/1‑244 (MQ=255)                                                                                                                                       gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                   >  1:188738/5‑251 (MQ=255)                                                                                                                                       gacctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTaaaaa                                                                                   >  1:669865/5‑251 (MQ=255)                                                                                                                                        acctACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTAGATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAAt                                                                                  <  1:450901/248‑1 (MQ=255)                                                                                                                                             cTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCAt                                                                                                                                                                          <  1:135188/158‑1 (MQ=255)                                                                                                                                               cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                                                                                                                                                             >  1:430895/1‑169 (MQ=255)                                                                                                                                               cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGaga                                                                                                          >  1:467730/1‑218 (MQ=255)                                                                                                                                               cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                           >  1:228148/1‑251 (MQ=255)                                                                                                                                               cacaTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCAtt                                                                           >  1:277073/1‑251 (MQ=255)                                                                                                                                                 caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGcc                                                                                                                         >  1:821727/1‑203 (MQ=255)                                                                                                                                                 caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                         >  1:421895/1‑251 (MQ=255)                                                                                                                                                 caTGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCa                                                                         >  1:410129/1‑251 (MQ=255)                                                                                                                                                        aggTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAg                                                                   <  1:857298/248‑1 (MQ=255)                                                                                                                                                             aTGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATg                                                                                 <  1:552000/231‑1 (MQ=255)                                                                                                                                                                             aGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtata                                             >  1:665540/1‑245 (MQ=255)                                                                                                                                                                               gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATActg                                                                                                                  <  1:144242/180‑3 (MQ=255)                                                                                                                                                                               gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtatat                                            <  1:623153/250‑8 (MQ=255)                                                                                                                                                                                 aTTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                         <  1:491142/251‑1 (MQ=255)                                                                                                                                                                                  ttGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTtacat                                                                                                                                   >  1:859494/1‑157 (MQ=255)                                                                                                                                                                                   tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                       <  1:744739/251‑1 (MQ=255)                                                                                                                                                                                   tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                       <  1:400564/251‑1 (MQ=255)                                                                                                                                                                                          aTGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGcc                                                                                                                                                                                                      <  1:439411/85‑1 (MQ=255)                                                                                                                                                                                          aTGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACg                                 >  1:657619/1‑250 (MQ=255)                                                                                                                                                                                                  ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGAc                                                                                                                                                                                  >  1:412830/9‑97 (MQ=255)                                                                                                                                                                                                  ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGaga                                                                                                          >  1:176733/9‑167 (MQ=255)                                                                                                                                                                                                  ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                         <  1:39444/226‑1 (MQ=255)                                                                                                                                                                                                   gattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTG‑CGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCa                      >  1:193324/8‑251 (MQ=255)                                                                                                                                                                                                       acgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                          <  1:592328/225‑1 (MQ=255)                                                                                                                                                                                                                 aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc         <  1:629036/251‑1 (MQ=255)                                                                                                                                                                                                                 aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc         <  1:93247/251‑1 (MQ=255)                                                                                                                                                                                                                   ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt       >  1:425820/1‑251 (MQ=255)                                                                                                                                                                                                                   ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt       >  1:87745/1‑251 (MQ=255)                                                                                                                                                                                                                    gTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGt                                                                  <  1:518623/191‑1 (MQ=255)                                                                                                                                                                                                                        gAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATg                                        <  1:792671/213‑1 (MQ=255)                                                                                                                                                                                                                         aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga  <  1:604529/250‑4 (MQ=255)                                                                                                                                                                                                                         aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga  <  1:545586/250‑4 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                           GGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCTTCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAG  >  1/1000270‑1000735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑