Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,359	A→T	intergenic (+49785/+5806)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,359	0	A	T	100.0%	135.2 / NA	51	intergenic (+49785/+5806)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (28/23);  total (28/23)

TTCCAGCCTTTTGAAAAGGCCTTTTGACCTTTTTAGGTAAGACCTTTCCCTTGGGAAGGTTATCCTTAAATTTTACCCTTAGCCCGATAGGGCTCCCTTGGGCCTTTTACCTTTTGGCAATTTTGTTGTATGATTCCAAGATAGATCATTATTACAATCCGCGCGCGTTGGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCTTCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTT  >  1/1000101‑1000602                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ttCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACtgaag                                                                                                                                                                                                                                                 <  1:150462/251‑1 (MQ=255)                               tttcaGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCttg                                                                                                                                                                                                                       >  1:702711/5‑246 (MQ=255)                               tttcaGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATg                                                                                                                                                                                                                   >  1:137420/5‑251 (MQ=255)                                                      gaaaGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTcaca                                                                                                                                                                                             <  1:768890/248‑1 (MQ=255)                                                      gaaaGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTcaca                                                                                                                                                                                             <  1:708263/248‑1 (MQ=255)                                                          ggTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTACGAcaaagctacg                                                                                                                                                                                             >  1:164276/1‑237 (MQ=255)                                                            ttATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGag                                                                                                                                                                                      <  1:692483/251‑2 (MQ=255)                                                              aTCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGaggt                                                                                                                                                                                    <  1:109841/251‑4 (MQ=255)                                                                                                                                                   ttcaaggtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTGCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGAt                                                                                                           <  1:451424/231‑1 (MQ=255)                                                                                                                                                         gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGAc                                                                                                                                                                                                      <  1:873354/140‑1 (MQ=255)                                                                                                                                                         gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATg                                                                                                                                                                                <  1:728756/162‑1 (MQ=255)                                                                                                                                                         gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACaggattac                                                                                                                                       <  1:227763/203‑8 (MQ=255)                                                                                                                                                         gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACaggatt                                                                                                                                         <  1:277116/201‑6 (MQ=255)                                                                                                                                                         gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAg                                                                                                      >  1:745072/15‑250 (MQ=255)                                                                                                                                                         gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAg                                                                                                      >  1:741916/15‑250 (MQ=255)                                                                                                                                                          tatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGc                                                                                                     <  1:128175/237‑1 (MQ=255)                                                                                                                                                           atgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCtt                                                                                                   >  1:479013/13‑251 (MQ=255)                                                                                                                                                                gatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGa                                                                                              >  1:633588/8‑251 (MQ=255)                                                                                                                                                                 atccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGAt                                                                                             <  1:304981/245‑1 (MQ=255)                                                                                                                                                                     gtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAg                                                                                         >  1:852226/3‑251 (MQ=255)                                                                                                                                                                          ggAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGaa                                                                                    >  1:481236/1‑251 (MQ=255)                                                                                                                                                                          ggAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGaa                                                                                    >  1:533506/1‑251 (MQ=255)                                                                                                                                                                                aaTATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                                              <  1:312984/251‑1 (MQ=255)                                                                                                                                                                                aaTATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                                              <  1:451611/251‑1 (MQ=255)                                                                                                                                                                                   atGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                                              <  1:667739/248‑1 (MQ=255)                                                                                                                                                                                   atGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTa                                                                            >  1:482085/1‑250 (MQ=255)                                                                                                                                                                                                    attaaaaaaTGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGaaa                                                                                                                                                        >  1:701368/4‑156 (MQ=255)                                                                                                                                                                                                       aaaaaaTGAGCAAACTCACTTTCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGa                                                       <  1:664728/251‑1 (MQ=255)                                                                                                                                                                                                                   aactcactttCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACaa                                           <  1:847551/243‑1 (MQ=255)                                                                                                                                                                                                                             cAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                       >  1:547591/1‑245 (MQ=255)                                                                                                                                                                                                                                    gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          >  1:776313/1‑251 (MQ=255)                                                                                                                                                                                                                                    gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          >  1:848303/1‑251 (MQ=255)                                                                                                                                                                                                                                    gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          >  1:690848/1‑251 (MQ=255)                                                                                                                                                                                                                                     agtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          <  1:345053/246‑1 (MQ=255)                                                                                                                                                                                                                                      gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCaaagta                                                           <  1:565035/213‑4 (MQ=255)                                                                                                                                                                                                                                      gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGa                                  <  1:574803/238‑1 (MQ=255)                                                                                                                                                                                                                                      gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                        >  1:380722/4‑251 (MQ=255)                                                                                                                                                                                                                                      gtattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                        >  1:686141/4‑251 (MQ=255)                                                                                                                                                                                                                                       tattATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATagag                        <  1:87181/248‑1 (MQ=255)                                                                                                                                                                                                                                               gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACaggattacgt                                                                                                                                     >  1:24671/2‑124 (MQ=255)                                                                                                                                                                                                                                               gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTc                    >  1:24566/2‑246 (MQ=255)                                                                                                                                                                                                                                               gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCa               >  1:282192/2‑251 (MQ=255)                                                                                                                                                                                                                                               gtGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCa               >  1:267620/2‑251 (MQ=255)                                                                                                                                                                                                                                                          gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACaggattacgt                                                                                                                                     <  1:240240/121‑10 (MQ=255)                                                                                                                                                                                                                                                          gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATgg                                                                                                                           >  1:695953/2‑132 (MQ=255)                                                                                                                                                                                                                                                          gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc                                       >  1:615472/2‑216 (MQ=255)                                                                                                                                                                                                                                                          gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          >  1:166077/2‑229 (MQ=255)                                                                                                                                                                                                                                                          gtCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          >  1:142115/2‑229 (MQ=255)                                                                                                                                                                                                                                                            ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATgg                                                                                                                           <  1:806915/130‑1 (MQ=255)                                                                                                                                                                                                                                                            ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag                          <  1:560529/227‑1 (MQ=255)                                                                                                                                                                                                                                                            ccTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGtt  >  1:839028/1‑251 (MQ=255)                                                                                                                                                                                                                                                               tAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTacacac                                                                       >  1:7649/1‑179 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    TTCCAGCCTTTTGAAAAGGCCTTTTGACCTTTTTAGGTAAGACCTTTCCCTTGGGAAGGTTATCCTTAAATTTTACCCTTAGCCCGATAGGGCTCCCTTGGGCCTTTTACCTTTTGGCAATTTTGTTGTATGATTCCAAGATAGATCATTATTACAATCCGCGCGCGTTGGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCTTCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTT  >  1/1000101‑1000602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑