Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,332	T→A	intergenic (+49758/+5833)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,332	0	T	A	100.0%	127.0 / NA	47	intergenic (+49758/+5833)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/28);  total (19/28)

AAAACGAACAAATATAGTGAGTGTAGTTCCAGCCTTTTGAAAAGGCCTTTTGACCTTTTTAGGTAAGACCTTTCCCTTGGGAAGGTTATCCTTAAATTTTACCCTTAGCCCGATAGGGCTCCCTTGGGCCTTTTACCTTTTGGCAATTTTGTTGTATGATTCCAAGATAGATCATTATTACAATCCGCGCGCGTTGGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCT‑TCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAG  >  1/1000075‑1000578                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       aaaacagataaatatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATc                                                                                                                                                                                                                                                   <  1:307693/226‑1 (MQ=255)       gataaatatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCA‑CTCTCAAGAAAGAGTATTATCAGTGtt                                                                                                                                                                                                                                             >  1:800660/20‑251 (MQ=255)            atatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                                                                                                                                                                                                                                        <  1:143591/237‑3 (MQ=255)            atatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                                                                                                                                                                                                                                        <  1:392502/237‑3 (MQ=255)                      ggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCag                                                                                                                                                                                                                                                 >  1:239658/5‑231 (MQ=255)                       gttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAAc                                                                                                                                                                                                                              <  1:11348/247‑1 (MQ=255)                       gttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAAc                                                                                                                                                                                                                              <  1:612114/247‑1 (MQ=255)                           ttCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACtgaag                                                                                                                                                                                                                         <  1:150462/251‑1 (MQ=255)                                                        ttttcAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGtattat                                                                                                                                                                                                                                                    >  1:609691/1‑195 (MQ=255)                                                         tttcaGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATc                                                                                                                                                                                                                                                   <  1:99293/191‑1 (MQ=255)                                                         tttcaGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCttg                                                                                                                                                                                               >  1:702711/5‑246 (MQ=255)                                                         tttcaGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATg                                                                                                                                                                                           >  1:137420/5‑251 (MQ=255)                                                                   gACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATc                                                                                                                                                                                                                                                   <  1:117744/185‑1 (MQ=255)                                                                              tggaaaGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                                                                                                                                                                                                                                        >  1:144612/5‑183 (MQ=255)                                                                               ggaaaGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                                                                                                                                                                                                                                        <  1:589814/181‑3 (MQ=255)                                                                                gaaaGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTcaca                                                                                                                                                                     <  1:768890/248‑1 (MQ=255)                                                                                gaaaGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTcaca                                                                                                                                                                     <  1:708263/248‑1 (MQ=255)                                                                                    ggTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATc                                                                                                                                                                                                                                                   <  1:73243/168‑1 (MQ=255)                                                                                    ggTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTACGAcaaagctacg                                                                                                                                                                     >  1:164276/1‑237 (MQ=255)                                                                                      ttATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGag                                                                                                                                                              <  1:692483/251‑2 (MQ=255)                                                                                        aTCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGaggt                                                                                                                                                            <  1:109841/251‑4 (MQ=255)                                                                                           cTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                                                                                                                                                                                                                                        <  1:254176/172‑3 (MQ=255)                                                                                                                                                                             ttcaaggtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTGCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGAt                                                                                   <  1:451424/231‑1 (MQ=255)                                                                                                                                                                                   gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGAc                                                                                                                                                                              <  1:873354/140‑1 (MQ=255)                                                                                                                                                                                   gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATg                                                                                                                                                        <  1:728756/162‑1 (MQ=255)                                                                                                                                                                                   gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACaggattac                                                                                                               <  1:227763/203‑8 (MQ=255)                                                                                                                                                                                   gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACaggatt                                                                                                                 <  1:277116/201‑6 (MQ=255)                                                                                                                                                                                   gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAg                                                                              >  1:745072/15‑250 (MQ=255)                                                                                                                                                                                   gtatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAg                                                                              >  1:741916/15‑250 (MQ=255)                                                                                                                                                                                    tatgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGc                                                                             <  1:128175/237‑1 (MQ=255)                                                                                                                                                                                     atgtagatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCtt                                                                           >  1:479013/13‑251 (MQ=255)                                                                                                                                                                                          gatccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGa                                                                      >  1:633588/8‑251 (MQ=255)                                                                                                                                                                                           atccgtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGAt                                                                     <  1:304981/245‑1 (MQ=255)                                                                                                                                                                                               gtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                                                                                                                                                                                                                                        <  1:277918/82‑3 (MQ=255)                                                                                                                                                                                               gtgTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAg                                                                 >  1:852226/3‑251 (MQ=255)                                                                                                                                                                                                    ggAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGaa                                                            >  1:533506/1‑251 (MQ=255)                                                                                                                                                                                                    ggAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGaa                                                            >  1:481236/1‑251 (MQ=255)                                                                                                                                                                                                          aaTATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                      <  1:312984/251‑1 (MQ=255)                                                                                                                                                                                                          aaTATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                      <  1:451611/251‑1 (MQ=255)                                                                                                                                                                                                             atGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATAtt                                                      <  1:667739/248‑1 (MQ=255)                                                                                                                                                                                                             atGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTa                                                    >  1:482085/1‑250 (MQ=255)                                                                                                                                                                                                                              attaaaaaaTGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGaaa                                                                                                                                >  1:701368/4‑156 (MQ=255)                                                                                                                                                                                                                                 aaaaaaTGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGa                               <  1:664728/251‑1 (MQ=255)                                                                                                                                                                                                                                             aactcactt‑tCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACaa                   <  1:847551/243‑1 (MQ=255)                                                                                                                                                                                                                                                        cAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTc               >  1:547591/1‑245 (MQ=255)                                                                                                                                                                                                                                                               gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag  >  1:690848/1‑251 (MQ=255)                                                                                                                                                                                                                                                               gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag  >  1:776313/1‑251 (MQ=255)                                                                                                                                                                                                                                                               gagTATTATCAGTGTTTGAACAGTCCTTAACTGAAGAGCAACGCTTTTTACAAGGTGTCTTGAATGGCAAAATTAAGACCTACTCACATGAAGAGGTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATag  >  1:848303/1‑251 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       AAAACGAACAAATATAGTGAGTGTAGTTCCAGCCTTTTGAAAAGGCCTTTTGACCTTTTTAGGTAAGACCTTTCCCTTGGGAAGGTTATCCTTAAATTTTACCCTTAGCCCGATAGGGCTCCCTTGGGCCTTTTACCTTTTGGCAATTTTGTTGTATGATTCCAAGATAGATCATTATTACAATCCGCGCGCGTTGGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCT‑TCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAGAGCAGCGCTTTTTACAAGGTGTCTTAAATGGAAAAATTAAGACTTACTCACATGAAGATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAG  >  1/1000075‑1000578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑