Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,105	A→T	intergenic (+49531/+6060)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,105	0	A	T	100.0%	86.7 / NA	33	intergenic (+49531/+6060)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/16);  total (17/16)

CACCGACAGTGTTGGCTGGGTAGAAATACCAAGGGATTCGGTATTACTTTTTTTACTTAGCATTTTATGAAAAATAGAAAACATTATAGTTGCTTCATGTTTTAGTAAGGGGAACTGTTTAATGTTGCAGAATACAAGTGATGGACATCAAAACAACAAATAAGTGATCATTAAAAACGAACAAATATAGTGAGTGTAGTTCCAGCCTTTTGAAAAGGCCTTTTGACCTTTTTAGGTAAGACCTTTCCCTTGGGAAGGTTATCCTTAAATTTTACCCTTAGCCCGATAGGGCTCCCTTGGGCCTTTTACCTTTTGGCAATTTTGTTGTATGATTCCAAGATAGATCATTATTACAATCCGCGCGCGTTGGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCT‑TCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAG  >  1/999902‑1000363                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                    cattggtaGTGTCGGTTGGATAGTAATATCAAAGGATTCGGTATTAGATTTTTTACTTAGGATTTTATGAAAAATGGAAAAC‑‑‑ATAGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTgga                                                                                                                                                                                                                   >  1:787157/8‑250 (MQ=255)                  ggatagtaaTATCAAAGGATTCGGTATTAGATTTTTTACTTAGGATTTTATGAAAAATGGAAAAC‑‑‑ATAGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAAtt                                                                                                                                                                                                  >  1:839919/8‑251 (MQ=255)                        taaTATCAAAGGATTCGGTATTAGATTTTTTACTTAGGATTTTATGAAAAATGGAAAAC‑‑‑ATAGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTAccc                                                                                                                                                                                            <  1:131650/250‑1 (MQ=255)                             tcaaaggATTCGGTATTAGATTTTTTACTTAGGATTTTATGAAAAATGGAAAAC‑‑‑ATAGTTGCTTCATATCTTAGTAAGGGGGGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGc                                                                                                                                                                                       <  1:442552/246‑1 (MQ=255)                                         gtattagattttttACTTAGGATTTTATGAAAAATGGAAAAC‑‑‑ATAGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCgct                                                                                                                                                                                  >  1:98711/9‑242 (MQ=255)                                                   ttttACTTAGGATTTTATGAAAAATGGAAAAC‑‑‑ATAGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTgttcct                                                                                                                                                                 <  1:416088/251‑6 (MQ=255)                                                                             atggaaaacataGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTttgttg                                                                                                                                          >  1:234863/10‑251 (MQ=255)                                                                                    acataGTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGAtt                                                                                                                                   >  1:210347/3‑251 (MQ=255)                                                                                         gTTGCTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAag                                                                                                                              >  1:360243/1‑251 (MQ=255)                                                                                             cTTCATATCTTAGTAAGGGGTGTTATTTAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtat                                                                                                                          >  1:190343/1‑247 (MQ=255)                                                                                                     cttagtaaggggtgttatttAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAg                                                                                                                                                                                >  1:572970/18‑189 (MQ=255)                                                                                                       tagtaaggggtgttatttAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGc                                                                                                                                                                             >  1:63624/16‑190 (MQ=255)                                                                                                                  gttatttAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtatgt                                                                                                                        >  1:197498/5‑226 (MQ=255)                                                                                                                     atttAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtatgtagatccgtgttggag                                                                                                         <  1:690409/243‑22 (MQ=255)                                                                                                                     atttAATGTTGCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtatgtagatc                                                                                                                   >  1:604511/2‑223 (MQ=255)                                                                                                                               gCAGAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAAtt                                                                                                                                                                                                  >  1:737542/1‑145 (MQ=255)                                                                                                                                  gAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGaaa                                                                                                                                                                                                                                                          <  1:774784/86‑1 (MQ=255)                                                                                                                                  gAATACAAGTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtatgtagatccgtgttggag                                                                                                         <  1:397000/231‑22 (MQ=255)                                                                                                                                          gTGATGAATATCAAAACAACAAATAAGTGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGtt                                                                                                                                                                                                             <  1:65838/123‑1 (MQ=255)                                                                                                                                                                     tGATCATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCaagaaag                                     <  1:651205/251‑1 (MQ=255)                                                                                                                                                                         cATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtatgtagatc                                                                                                                   >  1:792599/1‑171 (MQ=255)                                                                                                                                                                         cATTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGta                                 >  1:353378/1‑250 (MQ=255)                                                                                                                                                                          aTTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGa                                                                                                                                     <  1:582822/163‑1 (MQ=255)                                                                                                                                                                          aTTAAAAACAGATAAATATGGTGCGGGTTGTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAaggtatgtagatc                                                                                                                   <  1:660186/181‑12 (MQ=255)                                                                                                                                                                              aaaacagataaatatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATc                            <  1:307693/226‑1 (MQ=255)                                                                                                                                                                                    gataaatatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCA‑CTCTCAAGAAAGAGTATTATCAGTGtt                      >  1:800660/20‑251 (MQ=255)                                                                                                                                                                                         atatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                 <  1:392502/237‑3 (MQ=255)                                                                                                                                                                                         atatggtgcgggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGaac                 <  1:143591/237‑3 (MQ=255)                                                                                                                                                                                                   ggttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCag                          >  1:239658/5‑231 (MQ=255)                                                                                                                                                                                                    gttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAAc       <  1:612114/247‑1 (MQ=255)                                                                                                                                                                                                    gttgTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAAc       <  1:11348/247‑1 (MQ=255)                                                                                                                                                                                                       gTTCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGa                                                                                                                                     >  1:223244/1‑134 (MQ=255)                                                                                                                                                                                                        ttCCTGCCTTTTGAAAAAGCCTTTTGGCCTTTTCAGGTAAGACCTTTCCCTTGGAAAGGTTATCCTTAAATTTTACCCTTAGCCCGCTAGGGCTCCCTTGTTCCTTTTACCTTTTTGCGGTTTTGTTGTATGATTTCAAGGTA‑‑‑‑‑‑T‑GTA‑GATC‑‑‑‑CGTGTTGGAGAAAATATGTTAAATCAAGCTAAATTAAAAAATGAGCAAACTCACTT‑TCAAGAAAGAGTATTATCAGTGTTTGAACAGTCCTTAACtgaag  <  1:150462/251‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                    CACCGACAGTGTTGGCTGGGTAGAAATACCAAGGGATTCGGTATTACTTTTTTTACTTAGCATTTTATGAAAAATAGAAAACATTATAGTTGCTTCATGTTTTAGTAAGGGGAACTGTTTAATGTTGCAGAATACAAGTGATGGACATCAAAACAACAAATAAGTGATCATTAAAAACGAACAAATATAGTGAGTGTAGTTCCAGCCTTTTGAAAAGGCCTTTTGACCTTTTTAGGTAAGACCTTTCCCTTGGGAAGGTTATCCTTAAATTTTACCCTTAGCCCGATAGGGCTCCCTTGGGCCTTTTACCTTTTGGCAATTTTGTTGTATGATTCCAAGATAGATCATTATTACAATCCGCGCGCGTTGGAGAAAATATGTTAAATCAAGCTAAATCAAAAAATGGGCAAACTCATCT‑TCAAGAAAGAGTTTTATCAATGTTTGAGCAATCCTTAACAGAAG  >  1/999902‑1000363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑