Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	997,466	T→C	intergenic (+46892/+8699)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	997,466	0	T	C	100.0%	196.9 / NA	70	intergenic (+46892/+8699)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/36);  total (34/36)

GTGGTTATGTAGCCTTGACGATGTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGTCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGAAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATG  >  1/997224‑997691                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  gtggtgATGTAGCCTTGACGATGTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGa                                                                                                                                                                                                                           >  1:238040/1‑251 (MQ=255)       aTGTAGCCTTGACGATGTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGt                                                                                                                                                                                                                      >  1:635590/1‑250 (MQ=255)               ttGACGATGTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCaaaaa                                                                                                                                                                                                             <  1:877494/251‑1 (MQ=255)                     aTGTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAgtggt                                                                                                                                                                                                       >  1:686833/1‑251 (MQ=255)                       gTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAgtggtg                                                                                                                                                                                                      >  1:487404/1‑250 (MQ=255)                              tGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGAtt                                                                                                                                                                                                <  1:164832/249‑1 (MQ=255)                                                         gTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGc                                                                                                                                                                                                     >  1:543635/1‑217 (MQ=255)                                                                agagGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAACTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAg                                                                                                                                                             <  1:60136/250‑1 (MQ=255)                                                                    cgacctaaaaacgccggcAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGc                                                                                                                                                                                                     >  1:856494/18‑206 (MQ=255)                                                                     cATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGa                                                                                                                                                       >  1:774171/1‑251 (MQ=255)                                                                      aTTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGa                                                                                                                                                       >  1:538848/1‑250 (MQ=255)                                                                       ttAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACa                                                                                                                                                     <  1:655129/251‑1 (MQ=255)                                                                        tAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACAt                                                                                                                                                    <  1:758811/251‑1 (MQ=255)                                                                                 cTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGA‑TTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCtat                                                                                                                                          >  1:529072/1‑251 (MQ=255)                                                                                   gCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCtata                                                                                                                                         >  1:7214/1‑251 (MQ=255)                                                                                    ccAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTatat                                                                                                                                        <  1:656064/251‑1 (MQ=255)                                                                                    ccAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTatat                                                                                                                                        <  1:603770/251‑1 (MQ=255)                                                                                    ccAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTatat                                                                                                                                        <  1:454702/251‑1 (MQ=255)                                                                                       aaaTCCGCCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtga                                                                                                                                     <  1:798934/251‑1 (MQ=255)                                                                                       aaaTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtga                                                                                                                                     <  1:289733/251‑1 (MQ=255)                                                                                       aaaTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtga                                                                                                                                     <  1:553248/251‑1 (MQ=255)                                                                                         aTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:711917/251‑1 (MQ=255)                                                                                         aTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:138925/251‑1 (MQ=255)                                                                                         aTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:465565/251‑1 (MQ=255)                                                                                         aTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:761125/251‑1 (MQ=255)                                                                                         aTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:895823/251‑1 (MQ=255)                                                                                         aTCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:505695/251‑1 (MQ=255)                                                                                           ccACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:512203/249‑1 (MQ=255)                                                                                                   cTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATgg                                                                                                                         <  1:399890/251‑1 (MQ=255)                                                                                                         gTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTCTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTgcg                                                                                                                                              >  1:614676/1‑224 (MQ=255)                                                                                                                 caacaaAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGaa                                                                                                                                                               <  1:626598/199‑1 (MQ=255)                                                                                                                 caacaaAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCAAGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTAt                                                                                                           >  1:195490/1‑251 (MQ=255)                                                                                                                    caaAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   >  1:277612/1‑224 (MQ=255)                                                                                                                           gaTCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAAc                                                                                                    >  1:133314/1‑248 (MQ=255)                                                                                                                            aTCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGc                                                                                                                                                                         <  1:490026/178‑1 (MQ=255)                                                                                                                              cTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTatat                                                                                                                                        >  1:127397/1‑209 (MQ=255)                                                                                                                               ttATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTatat                                                                                                                                        >  1:93409/1‑208 (MQ=255)                                                                                                                               ttATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   >  1:217103/1‑213 (MQ=255)                                                                                                                                      gCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCtat                                                                                                                                          >  1:643355/1‑199 (MQ=255)                                                                                                                                      gCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTatat                                                                                                                                        >  1:548981/1‑201 (MQ=255)                                                                                                                                      gCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   >  1:119974/1‑206 (MQ=255)                                                                                                                                      gCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAg                                                                                      >  1:758799/1‑251 (MQ=255)                                                                                                                                       cTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGt                                                                                     <  1:126423/251‑1 (MQ=255)                                                                                                                                             gTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCa                                                                                                                                                                       <  1:494091/163‑1 (MQ=255)                                                                                                                                             gTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTg                                                                                                <  1:317076/234‑1 (MQ=255)                                                                                                                                             gTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAgcag                                                                                >  1:814019/1‑247 (MQ=255)                                                                                                                                             gTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAgcag                                                                                >  1:626935/1‑247 (MQ=255)                                                                                                                                             gTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAgcag                                                                                >  1:237368/1‑247 (MQ=255)                                                                                                                                              tttACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGcc                                                                              <  1:541069/251‑1 (MQ=255)                                                                                                                                              tttACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGcc                                                                              <  1:76934/251‑1 (MQ=255)                                                                                                                                              tttACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGcc                                                                              <  1:338736/251‑1 (MQ=255)                                                                                                                                                  cGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGt                                                                          >  1:92905/1‑251 (MQ=255)                                                                                                                                                    aCCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTa                                                                        >  1:547509/1‑251 (MQ=255)                                                                                                                                                        ttGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGc                                                                    <  1:28199/251‑1 (MQ=255)                                                                                                                                                        ttGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGc                                                                    <  1:508548/251‑1 (MQ=255)                                                                                                                                                        ttGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGc                                                                    <  1:181372/251‑1 (MQ=255)                                                                                                                                                        ttGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGATCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGc                                                                    <  1:880305/251‑1 (MQ=255)                                                                                                                                                             cGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGt                                                                >  1:206529/1‑250 (MQ=255)                                                                                                                                                                     gtgcgCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGcc                                                                                                                                                                   <  1:867294/143‑1 (MQ=255)                                                                                                                                                                                cATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAatcatc                                            <  1:587920/251‑1 (MQ=255)                                                                                                                                                                                  tGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtga                                                                                                                                     >  1:321433/1‑160 (MQ=255)                                                                                                                                                                                                      gTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAAc                                                                                                    <  1:736351/173‑1 (MQ=255)                                                                                                                                                                                                          ccGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTa                  >  1:366682/1‑251 (MQ=255)                                                                                                                                                                                                                  cTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAg                                                                                      <  1:374309/175‑1 (MQ=255)                                                                                                                                                                                                                  cTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGc           >  1:211888/1‑250 (MQ=255)                                                                                                                                                                                                                  cTCGTGACCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGc           >  1:474985/1‑250 (MQ=255)                                                                                                                                                                                                                        aCCATCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTAcc                        >  1:4271/1‑231 (MQ=255)                                                                                                                                                                                                                           aTCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGc              >  1:844464/1‑238 (MQ=255)                                                                                                                                                                                                                           aTCTTAAATGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATg  >  1:338484/1‑250 (MQ=255)                                                                                                                                                                                                                                aaaTGATGACGCGGAAACGCCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAGACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATtgatg                                                                                                                                   <  1:156949/116‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  GTGGTTATGTAGCCTTGACGATGTAAAAGTGCGGTGGATTTTGCCGGCGTTTTTAGGTCGTGGAGAGGCATTAAAAAACACTGCCAAAATCCACCGCACTTTCCGTGAAAACCAACAAAGGAGATCTTATGAAGCTTTATGTTTACGACCATTGCCCGTTTTGCGTGCGCGCCCGCATGATTTTCGGCTTGAAAAATGTTCCCGTTGAACTCGTGACCATCTTAAATGATGACGCGGAAACGTCAATCCGACTGGTCGGCAAAAAAGTGGTGCCGATTTTAGTGAAAGAAAACGGCGAAGCCATGCCGGAAAGTTTGGACATTGTGCGCTATATTGATGAACGCTATGGCGAGAAAATGTTATCGGAAACGGTGCGCCCGGAAGTAGAAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATG  >  1/997224‑997691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑