Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	996,788	C→T	intergenic (+46214/+9377)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	996,788	0	C	T	100.0%	178.4 / NA	67	intergenic (+46214/+9377)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/30);  total (37/30)

AATCAACCGCTCTTTGATGTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGCATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTGGAAACCGTGTGCTATGAAAT  >  1/996549‑997033                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      aaTCAACCGCTCTTTGATGTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTAt                                                                                                                                                                                                                                            <  1:305950/251‑1 (MQ=255)                  tGTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATg                                                                                                                                                                                                                            >  1:105103/1‑250 (MQ=255)                  tGTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATg                                                                                                                                                                                                                            >  1:472461/1‑250 (MQ=255)                   gTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCa                                                                                                                                                                                                                          >  1:651282/1‑251 (MQ=255)                   gTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCa                                                                                                                                                                                                                          >  1:290039/1‑251 (MQ=255)                           cGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAaaga                                                                                                                                                                                                                    <  1:466978/249‑1 (MQ=255)                                aaaCGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAacac                                                                                                                                                                                                             <  1:369016/251‑1 (MQ=255)                                     ggCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCa                                                                                                                                                                                                         >  1:208858/1‑250 (MQ=255)                                       cATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCAcc                                                                                                                                                                                                       <  1:485461/250‑1 (MQ=255)                                       cATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCAcc                                                                                                                                                                                                       <  1:535726/250‑1 (MQ=255)                                          tCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCACAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGtatca                                                                                                                                                                                                                                                   >  1:775273/1‑203 (MQ=255)                                                    ttGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACTCCGCACCCGCGTGTTGGAATc                                                                                                                                                                                         <  1:153760/251‑1 (MQ=255)                                                      ggATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGtatcatcat                                                                                                                                                                                                                                               >  1:62578/1‑195 (MQ=255)                                                                   aaTCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATc                                                                                                                                                                                         <  1:816214/236‑1 (MQ=255)                                                                    aTCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGa                                                                                                                                                                                                                                >  1:54802/1‑196 (MQ=255)                                                                       tcAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGcc                                                                                                                                                                      <  1:613873/251‑1 (MQ=255)                                                                                        ttGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAAc                                                                                                                                                     <  1:169498/251‑1 (MQ=255)                                                                                         tGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAAcc                                                                                                                                                    <  1:46006/251‑1 (MQ=255)                                                                                                 gAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCaa                                                                                                                                            >  1:731482/1‑251 (MQ=255)                                                                                                  aaCAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAAcc                                                                                                                                                    >  1:27915/1‑242 (MQ=255)                                                                                                      gacagaAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGa                                                                                                                                                         <  1:108386/233‑1 (MQ=255)                                                                                                      gacagaAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAAcc                                                                                                                                                    >  1:543500/1‑238 (MQ=255)                                                                                                      gacagaAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGtt                                                                                                                                       >  1:621748/1‑251 (MQ=255)                                                                                                      gacagaAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGtt                                                                                                                                       >  1:33819/1‑251 (MQ=255)                                                                                                      gacagaAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGtt                                                                                                                                       >  1:790435/1‑251 (MQ=255)                                                                                                           aaGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGACGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCa                                                                                                                                    >  1:438652/1‑249 (MQ=255)                                                                                                               cATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTc                                                                                                                                 >  1:786270/1‑248 (MQ=255)                                                                                                               cATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGACATGCAAAAAGAAGAACACCGCACCcg                                                                                                                                                                                                     <  1:10190/180‑1 (MQ=255)                                                                                                                aTGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGtt                                                                                                                             >  1:20434/1‑251 (MQ=255)                                                                                                                     caccaTCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGc                                                                                                                        <  1:68318/251‑1 (MQ=255)                                                                                                                          tCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCtggtg                                                                                                                   <  1:229743/251‑1 (MQ=255)                                                                                                                             aCATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGaa                                                                                                                >  1:34492/1‑251 (MQ=255)                                                                                                                             aCATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGaa                                                                                                                >  1:213025/1‑251 (MQ=255)                                                                                                                               aTTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAAt                                                                                                              <  1:165160/251‑1 (MQ=255)                                                                                                                                ttAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGTCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATg                                                                                                             <  1:430335/251‑1 (MQ=255)                                                                                                                                      ccGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCg                                                                                                        >  1:454199/1‑250 (MQ=255)                                                                                                                                         gcgcTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCt                                                                                                                       >  1:84534/1‑232 (MQ=255)                                                                                                                                              ttCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCt                                                                                                                       >  1:679771/1‑227 (MQ=255)                                                                                                                                              ttCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCa                                                                                                >  1:241637/1‑250 (MQ=255)                                                                                                                                                                         aCCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATg                                                                    >  1:176003/1‑251 (MQ=255)                                                                                                                                                                          ccATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGAcc                                                                                                          >  1:846027/1‑212 (MQ=255)                                                                                                                                                                            aTTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTc                                                                                                                                 >  1:778552/1‑187 (MQ=255)                                                                                                                                                                                 cAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTGATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGAtt                                                            <  1:430093/251‑1 (MQ=255)                                                                                                                                                                                    tACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGc                                                          >  1:55971/1‑250 (MQ=255)                                                                                                                                                                                         aCATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGc                                                                                                                        >  1:61056/1‑183 (MQ=255)                                                                                                                                                                                         aCATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGc                                                     >  1:777089/1‑250 (MQ=255)                                                                                                                                                                                                 gCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCACGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAgg                                            >  1:534201/1‑251 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGc                                                                        <  1:671644/222‑1 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAggg                                           <  1:403984/251‑1 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAggg                                           <  1:400583/251‑1 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAggg                                           <  1:519805/251‑1 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAggg                                           <  1:726362/251‑1 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAg                                             <  1:541539/249‑1 (MQ=255)                                                                                                                                                                                                  cTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAg                                             <  1:689591/249‑1 (MQ=255)                                                                                                                                                                                                    aCCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCg                                         >  1:49067/1‑251 (MQ=255)                                                                                                                                                                                                        aaGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGa                                     >  1:627353/1‑251 (MQ=255)                                                                                                                                                                                                          gCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACg                                   >  1:134980/1‑251 (MQ=255)                                                                                                                                                                                                            cATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCCCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACggg                                 <  1:899690/251‑1 (MQ=255)                                                                                                                                                                                                              tCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAGATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTg                               >  1:439094/1‑251 (MQ=255)                                                                                                                                                                                                                   cAACAGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATGTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCGTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACAAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAAc                          <  1:426307/251‑1 (MQ=255)                                                                                                                                                                                                                       aGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGa                                                              <  1:348430/211‑1 (MQ=255)                                                                                                                                                                                                                       aGCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTg                      >  1:449072/1‑251 (MQ=255)                                                                                                                                                                                                                        gCTCCAACTGCGCAATCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCGAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTgg                     >  1:454783/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTCTCGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGGGAAAACCGTGGAAACCGTGTGc          >  1:723151/1‑248 (MQ=255)                                                                                                                                                                                                                                       tctcGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTGGAAACCGTGTGCTATg      <  1:82200/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGccc                                                        <  1:542113/198‑1 (MQ=255)                                                                                                                                                                                                                                          cGGTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTGGAAACCGTGTGCTATGaaa   >  1:493072/1‑251 (MQ=255)                                                                                                                                                                                                                                            gTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTc                                                                                                                                 >  1:421076/1‑123 (MQ=255)                                                                                                                                                                                                                                            gTGGTATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTGGAAACCGTGTGCTATGAAAt  <  1:708046/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      AATCAACCGCTCTTTGATGTCTATAGCGTGGAAACGGGCATTCATAACGCGTTGGATAAACGGGTGAATCTCAAATCCGGTGGCTATTTGATTATTGAACAGACAGAAGCCATGACCACCATCGACATTAACACCGGCGCTTTCGTGGGGCATCGCAATCTGGAAGAAACCATTTTCAATACCAACATCGAAGCTACCAAAGCCATCGCCCAACAGCTCCAACTGCGCAATCTCGGTGGCATCATCATTATCGATTTTATCGATATGCAAAAAGAAGAACACCGCACCCGCGTGTTGGAATCCTTGCAGGAAGCCCTTGCCGGCGACCCGGTGAAAACCAATGTCAATGGTTTCACTCAGTTGGGGCTGGTGGAAATGACCCGCAAACGCACCCGCGAAAGTTTGGAGCGCGTGCTATGCGGCGATTGCCCCGCCTGCCAAGGGCGTGGACGGGTGAAAACCGTGGAAACCGTGTGCTATGAAAT  >  1/996549‑997033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑