Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	993,935	T→C	intergenic (+43361/+12230)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	993,935	0	T	C	100.0%	218.5 / NA	77	intergenic (+43361/+12230)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/34);  total (43/34)

CATCTAAAATAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCTAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGA  >  1/993706‑994181                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cATCTAAAATAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAg                                                                                                                                                                                                                                   >  1:372006/1‑251 (MQ=255)   tCTAAAATAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGa                                                                                                                                                                                                                                  >  1:575254/1‑250 (MQ=255)   tCTAAAATAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGAc                                                                                                                                                                                                                                 <  1:798578/251‑1 (MQ=255)   tCTAAAATAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGAc                                                                                                                                                                                                                                 <  1:561104/251‑1 (MQ=255)       aaaTAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACtttt                                                                                                                                                                                                                             >  1:502714/1‑251 (MQ=255)           aatGCGATCCTGCAACGATGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGccc                                                                                                                                                                                                                         <  1:345678/251‑1 (MQ=255)            atGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTc                                                                                                                                                                                                                                       >  1:257242/1‑236 (MQ=255)                 aTCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTgg                                                                                                                                                                                                                   >  1:223139/1‑251 (MQ=255)                    cTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCAt                                                                                                                                                                                                                <  1:705495/251‑1 (MQ=255)                           ggTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGt                                                                                                                                                                                                                                                    >  1:831463/1‑208 (MQ=255)                            gTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGAc                                                                                                                                                                                                                                 <  1:301557/226‑1 (MQ=255)                            gTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCAt                                                                                                                                                                                                                >  1:679240/1‑243 (MQ=255)                               cAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATg                                                                                                                                                                                                      <  1:495612/250‑1 (MQ=255)                                 aGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGGCCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAg                                                                                                                                                                                                                                             <  1:630835/209‑1 (MQ=255)                                   aTGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGtt                                                                                                                                                                                                  <  1:699423/250‑1 (MQ=255)                                      gggCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGAc                                                                                                                                                                                                                                 >  1:209982/1‑216 (MQ=255)                                        gcagcaCGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTttgttg                                                                                                                                                                                             <  1:828691/250‑1 (MQ=255)                                           gcaCGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTtgctg                                                                                                                                                                                          >  1:639968/1‑250 (MQ=255)                                           gcaCGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTtgctg                                                                                                                                                                                          >  1:30355/1‑250 (MQ=255)                                           gcaCGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTtgctg                                                                                                                                                                                          >  1:662770/1‑250 (MQ=255)                                             aCGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCa                                                                                                                                                                                       <  1:15997/251‑1 (MQ=255)                                              cgcgATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCAt                                                                                                                                                                                      >  1:418970/1‑251 (MQ=255)                                                                         aTTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGt                                                                                                                                                                                                                                                    <  1:471951/162‑1 (MQ=255)                                                                            cGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGc                                                                                                                                                        <  1:117559/251‑1 (MQ=255)                                                                              cAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGcc                                                                                                                                                       <  1:246248/250‑1 (MQ=255)                                                                                aTCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATg                                                                                                                                                    >  1:301195/1‑251 (MQ=255)                                                                                         aaTGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAgg                                                                                                                                            <  1:189679/250‑1 (MQ=255)                                                                                                 gCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTtggtggtg                                                                                                                                   >  1:81304/1‑251 (MQ=255)                                                                                                     aTCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATggg                                                                                                                                                                                                    <  1:251941/182‑1 (MQ=255)                                                                                                      tCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGAt                                                                                                                              >  1:841725/1‑251 (MQ=255)                                                                                                             ggCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGa                                                                                                                       >  1:618999/1‑251 (MQ=255)                                                                                                                cTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATaa                                                                                                                    >  1:823805/1‑251 (MQ=255)                                                                                                                           ggCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAg                                                                                                                >  1:620114/1‑244 (MQ=255)                                                                                                                             cATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAAt                                                                                                       <  1:647333/251‑1 (MQ=255)                                                                                                                                gAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGcc                                                                                                                                                       >  1:274668/1‑200 (MQ=255)                                                                                                                                   cTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCa                                                                                                 >  1:636064/1‑251 (MQ=255)                                                                                                                                     gTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGcc                                                                                                                                                                                        >  1:378799/1‑162 (MQ=255)                                                                                                                                     gTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCAtt                                                                                               >  1:70617/1‑251 (MQ=255)                                                                                                                                            gtggtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAt                                                                                        >  1:753683/1‑251 (MQ=255)                                                                                                                                            gtggtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAt                                                                                        >  1:149671/1‑251 (MQ=255)                                                                                                                                               gtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAAt                                                                                                       >  1:636593/1‑233 (MQ=255)                                                                                                                                               gtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAtct                                                                                      >  1:223412/1‑250 (MQ=255)                                                                                                                                               gtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGAGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAtct                                                                                      >  1:446111/1‑250 (MQ=255)                                                                                                                                                 gCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTttgttg                                                                                                                                                                                             <  1:895500/145‑1 (MQ=255)                                                                                                                                                 gCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAt                                                                                        >  1:588312/1‑246 (MQ=255)                                                                                                                                                 gCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCg                                                                                    >  1:448265/1‑250 (MQ=255)                                                                                                                                                   acaATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCCTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTtgc                                                                                                                                                                                            >  1:273496/1‑144 (MQ=255)                                                                                                                                                      aTACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTg                                                                                                                                                                                <  1:560594/153‑1 (MQ=255)                                                                                                                                                         cGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATaa                                                                             <  1:338400/249‑1 (MQ=255)                                                                                                                                                         cGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAg                                                                           <  1:541677/251‑1 (MQ=255)                                                                                                                                                           ttGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTg                                                                                                                                                                                    >  1:675400/1‑144 (MQ=255)                                                                                                                                                           ttGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGc                                                                          <  1:745497/250‑1 (MQ=255)                                                                                                                                                                     ccTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTttgttg                                                                                                                                                                                             <  1:17077/125‑1 (MQ=255)                                                                                                                                                                               gaggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATaa                                                                             >  1:764385/1‑227 (MQ=255)                                                                                                                                                                               gaggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTggg                                                      >  1:147656/1‑250 (MQ=255)                                                                                                                                                                                aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTggg                                                      <  1:301006/249‑1 (MQ=255)                                                                                                                                                                                aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                     <  1:187044/250‑1 (MQ=255)                                                                                                                                                                                aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                     <  1:373850/250‑1 (MQ=255)                                                                                                                                                                                aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                     <  1:273502/250‑1 (MQ=255)                                                                                                                                                                                          cTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAgg                                          <  1:540559/251‑1 (MQ=255)                                                                                                                                                                                          cTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAgg                                          <  1:198412/251‑1 (MQ=255)                                                                                                                                                                                           tttCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGgt                                         <  1:669408/251‑1 (MQ=255)                                                                                                                                                                                                gATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCgg                                    >  1:281299/1‑251 (MQ=255)                                                                                                                                                                                                 aTTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGt                                   <  1:564496/251‑1 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCa                                                                                                                                                                                                                 >  1:180426/1‑64 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTg                                                                   <  1:638873/206‑1 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                       >  1:653468/1‑250 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                       >  1:896538/1‑250 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                       >  1:822055/1‑250 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                       >  1:361881/1‑250 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                       >  1:686019/1‑250 (MQ=255)                                                                                                                                                                                                              tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCCTCTTTGTACGCCTTTTGGGCTTTGTCAAAgg                                          >  1:316029/1‑231 (MQ=255)                                                                                                                                                                                                                 cAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGt                   <  1:900804/251‑1 (MQ=255)                                                                                                                                                                                                                        aTGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGc                                                              <  1:762026/201‑1 (MQ=255)                                                                                                                                                                                                                        aTGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                     <  1:537151/210‑1 (MQ=255)                                                                                                                                                                                                                             ggATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCAt                                                                                                >  1:434774/1‑162 (MQ=255)                                                                                                                                                                                                                             ggATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                     >  1:73379/1‑205 (MQ=255)                                                                                                                                                                                                                                  ctgccAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGa  >  1:804935/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CATCTAAAATAATGCGATCCTGCAACGGTGCAAGATGGGGCAGCACGCGATCCCATTTGAAATCGGAGCGCCATTCGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCTAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGA  >  1/993706‑994181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑