Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	989,994	C→T	intergenic (+39420/+16171)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	989,994	0	C	T	100.0%	185.3 / NA	68	intergenic (+39420/+16171)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/33);  total (35/33)

GTTTTGGCGTTGGCGTAAATAATTGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGCGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACAAGGCTTTGGCGTGATAATTTATTT  >  1/989752‑990211                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          gttttgGCGTTGGCGTAAATAATTGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAggg                                                                                                                                                                                                                   >  1:526915/1‑251 (MQ=255)            ggcgtAAATAATTGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTa                                                                                                                                                                                                        >  1:23879/1‑251 (MQ=255)            ggcgtAAATAATTGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTa                                                                                                                                                                                                        >  1:664082/1‑251 (MQ=255)              cgtAAATAATTGGCGATGCCAATGTCCGCCGCCGTTAATCCTGCGCGGGCATCCACAAAAAAGAGACCAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTAc                                                                                                                                                                                                       <  1:826177/250‑1 (MQ=255)                  aataatTGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTc                                                                                                                                                                                                  <  1:796424/251‑1 (MQ=255)                       ttGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACg                                                                                                                                                                                             <  1:546642/251‑1 (MQ=255)                              tGCCAGTGTCCGCGGACGTTATTCCTGCGCGGGCATCCACAAAAAAGAGACCAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAAt                                                                                                                                                                                      <  1:327437/251‑1 (MQ=255)                              tGCCAATGTCCGCCGCCGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAAt                                                                                                                                                                                      <  1:439550/251‑1 (MQ=255)                              tGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAAt                                                                                                                                                                                      >  1:720191/1‑251 (MQ=255)                              tGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAAt                                                                                                                                                                                      <  1:199260/251‑1 (MQ=255)                                ccAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAg                                                                                                                                                                                    <  1:496950/251‑1 (MQ=255)                                caaaTGTCCGCCGTCGTTAACCTTGCGCGGGCACCCACAAAAAAGAGACCAATTTCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAg                                                                                                                                                                                    <  1:862767/249‑1 (MQ=255)                                       ccgccgACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGTCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGAt                                                                                                                                                                             <  1:444534/251‑1 (MQ=255)                                            gACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGc                                                                                                                                                                         >  1:297623/1‑250 (MQ=255)                                              cGTTAATCCTGCGCGGGCATCCACCAAAAGGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGa                                                                                                                                                                      >  1:801422/1‑251 (MQ=255)                                              cGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGa                                                                                                                                                                      >  1:613963/1‑251 (MQ=255)                                              cGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGa                                                                                                                                                                      >  1:82044/1‑251 (MQ=255)                                              cGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGa                                                                                                                                                                      >  1:276249/1‑251 (MQ=255)                                                  aaTCCTGCGCGGGAATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGTCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACAtt                                                                                                                                                                  <  1:766478/251‑1 (MQ=255)                                                                   caccaAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAAt                                                                                                                                                                                      >  1:115266/1‑214 (MQ=255)                                                                   caccaAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAgg                                                                                                                                                 >  1:117280/1‑251 (MQ=255)                                                                    cccaAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGc                                                                                                                                                <  1:662048/250‑1 (MQ=255)                                                                    accaAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCCTTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGCGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAgg                                                                                                                                                 >  1:664396/1‑250 (MQ=255)                                                                                   atatCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGt                                                                                                                                 >  1:365025/1‑251 (MQ=255)                                                                                        cGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACggg                                                                                                                                       <  1:134975/240‑1 (MQ=255)                                                                                             ctTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCa                                                                                                                               >  1:683577/1‑243 (MQ=255)                                                                                             ctTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAAcc                                                                                                                       <  1:558079/251‑1 (MQ=255)                                                                                             ctTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAAc                                                                                                                        >  1:386854/1‑250 (MQ=255)                                                                                             ctTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAAc                                                                                                                        >  1:528090/1‑250 (MQ=255)                                                                                                          cagcGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATaaatga                                                                                                           <  1:615799/250‑1 (MQ=255)                                                                                                          cagcGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATaaatga                                                                                                           <  1:703919/250‑1 (MQ=255)                                                                                                            gtgATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTTGCTACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAACCGGTGACGTGGGCGTCGCGGGTACGGGTCAGTCGATTAAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTTATAAAAACCTCTCATaaatgaaat                                                                                                        >  1:454632/3‑251 (MQ=255)                                                                                                            gcGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAggg                                                                                                                                                                                                                   <  1:12780/144‑1 (MQ=255)                                                                                                             cGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATaaatgaaa                                                                                                         <  1:571456/249‑1 (MQ=255)                                                                                                                             ttttCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTg                                                                                       <  1:874423/251‑1 (MQ=255)                                                                                                                                 cttctACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGatat                                                                                   <  1:219671/251‑1 (MQ=255)                                                                                                                                      acacCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATaaatg                                                                                                            >  1:646821/1‑221 (MQ=255)                                                                                                                                      acacCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCa                                                                              >  1:410311/1‑251 (MQ=255)                                                                                                                                       cacCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCAc                                                                             <  1:488501/251‑1 (MQ=255)                                                                                                                                        acCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAg                                                                                                                                     >  1:269171/1‑194 (MQ=255)                                                                                                                                        acCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCa                                                                              >  1:231717/1‑249 (MQ=255)                                                                                                                                        acCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCa                                                                              >  1:617025/1‑249 (MQ=255)                                                                                                                                         ccTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTg                                                                                                                                  >  1:360552/1‑196 (MQ=255)                                                                                                                                             cttcCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCaa                                                                                                                                                   <  1:319224/175‑1 (MQ=255)                                                                                                                                             cttcCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGaaa                                                                       >  1:425296/1‑251 (MQ=255)                                                                                                                                               tcCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGaaaaa                                                                     <  1:849650/251‑1 (MQ=255)                                                                                                                                                  gTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAAc                                                                                                                        >  1:477247/1‑197 (MQ=255)                                                                                                                                                    gCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGt                                                                                                                                                                                                                <  1:620477/107‑1 (MQ=255)                                                                                                                                                        tCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACggcaggca                                                            <  1:248298/251‑1 (MQ=255)                                                                                                                                                           aTACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTg                                                                                                                                  >  1:436051/1‑178 (MQ=255)                                                                                                                                                              ccGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCGTGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAg                                                      <  1:655266/251‑1 (MQ=255)                                                                                                                                                              ccGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAg                                                      <  1:231153/251‑1 (MQ=255)                                                                                                                                                               cGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAg                                                      >  1:328078/1‑250 (MQ=255)                                                                                                                                                                gCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAAtgt                                                                                                      <  1:647527/201‑1 (MQ=255)                                                                                                                                                                     tATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGAttt                                               >  1:347716/1‑251 (MQ=255)                                                                                                                                                                     tATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGAttt                                               >  1:454175/1‑251 (MQ=255)                                                                                                                                                                         aaTGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGc                                           <  1:886551/251‑1 (MQ=255)                                                                                                                                                                         aaTGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGc                                           <  1:749356/251‑1 (MQ=255)                                                                                                                                                                              cGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAAc                                                                                                                        <  1:612056/169‑1 (MQ=255)                                                                                                                                                                                aTAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCatgatg                                    >  1:209316/1‑251 (MQ=255)                                                                                                                                                                                          ggCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACa                          >  1:756732/1‑251 (MQ=255)                                                                                                                                                                                                  aGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACAAGGCTTTg                  >  1:246535/1‑251 (MQ=255)                                                                                                                                                                                                            gACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAg                                                      >  1:420611/1‑205 (MQ=255)                                                                                                                                                                                                            gACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACAAGGc                      >  1:148282/1‑237 (MQ=255)                                                                                                                                                                                                             aCCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATa                                                                                                                <  1:118747/146‑1 (MQ=255)                                                                                                                                                                                                              ccATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACAAGGCTTTGGCg               <  1:711519/242‑1 (MQ=255)                                                                                                                                                                                                                 tatTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACa                          >  1:595848/1‑228 (MQ=255)                                                                                                                                                                                                                  atTTGCGATCGCGGGTTAAACCGGGAAAATCGGTGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACAAGGCTTTGGCGTGATAatttattt  <  1:651162/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          GTTTTGGCGTTGGCGTAAATAATTGGCGATGCCAATGTCCGCCGACGTTAATCCTGCGCGGGCATCCACCAAAAAGAGAACAATATCCGCCTCTTCAATGGCGAGCAGCGATTGCTCCGCCATTTTTTCTTCTACACCTTCTTCCGTGCCGTCGATACCGCCCGTATCAATGACGATAAAATCATGGCCGGCAAGGTGGGCATGACCATATTTGCGATCGCGGGTTAAACCGGGAAAATCGGCGACGAGGGCGTCGCGGGTACGGGTCAGACGATTGAATAGCGTGGATTTGCCGACATTCGGGCGACCCACCAAGGCAACTACGGGAGTTGTCATAAAAACCTCTCATAAATGAAATGTGCCACGGGAAGATTGATATCGGCACTTGAAAAACGGCAGGCATTATAGCGGATTTTAGCCATGATGAGAAATGACAAGGCTTTGGCGTGATAATTTATTT  >  1/989752‑990211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑