Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	986,276	C→T	intergenic (+35702/+19889)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	986,276	0	C	T	100.0%	188.0 / NA	70	intergenic (+35702/+19889)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/35);  total (35/35)

AAATACAAGAACCAACAAAATGCCGTTGCCAGCCGCATTGATGAGGACAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATCAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGCAACAATAAAATGTTTAAT  >  1/986031‑986522                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         aaaTACAAGAACCAACAAAATGCCGTTGCCAGCCGCATTGATGAGGACAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCAt                                                                                                                                                                                                                                                     >  1:39613/1‑251 (MQ=255)                                        gatgaGGACAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATcc                                                                                                                                                                                                                      >  1:643067/1‑243 (MQ=255)                                         atgaGGACAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGttt                                                                                                                                                                                                             <  1:747937/251‑1 (MQ=255)                                             ggACAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGtttt                                                                                                                                                                                                            >  1:430819/1‑248 (MQ=255)                                               aCAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTgg                                                                                                                                                                                                        <  1:424694/250‑1 (MQ=255)                                                       atGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTc                                                                                                                                                                                                                              >  1:53966/1‑220 (MQ=255)                                                         gAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAAt                                                                                                                                                                                             >  1:893615/1‑251 (MQ=255)                                                                    cAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGgcg                                                                                                                                                                                     >  1:77988/1‑248 (MQ=255)                                                                       ttattaCTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAAt                                                                                                                                                                                                                                 >  1:564316/1‑201 (MQ=255)                                                                       ttattaCTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGaa                                                                                                                                                                               >  1:79144/1‑251 (MQ=255)                                                                       ttattaCTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACACAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGaa                                                                                                                                                                               >  1:49149/1‑251 (MQ=255)                                                                           taCTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATcc                                                                                                                                                                           <  1:90128/251‑1 (MQ=255)                                                                                         tACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGAATTTAGCCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATgg                                                                                                                                                             >  1:897565/1‑251 (MQ=255)                                                                                                 aTCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTaaaaa                                                                                                                                                     <  1:786911/251‑1 (MQ=255)                                                                                                 aTCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTaaaaa                                                                                                                                                     <  1:493506/251‑1 (MQ=255)                                                                                                   cTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAAcg                                                                                                                                                   >  1:815511/1‑251 (MQ=255)                                                                                                           tttttGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAAt                                                                                                                                           <  1:425581/251‑1 (MQ=255)                                                                                                           tttttGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAAt                                                                                                                                           >  1:672051/1‑251 (MQ=255)                                                                                                           tttttGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAAt                                                                                                                                           <  1:550527/251‑1 (MQ=255)                                                                                                           tttttGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAAt                                                                                                                                           <  1:898553/251‑1 (MQ=255)                                                                                                                 aTGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑gcgc                                                                                                                                    <  1:831304/250‑1 (MQ=255)                                                                                                                 aTGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑gcgc                                                                                                                                    <  1:289853/250‑1 (MQ=255)                                                                                                                    tCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGat                                                                                                                                >  1:498271/1‑251 (MQ=255)                                                                                                                          ttCAGAAACTGGAAACGGTGGTTTCTGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGgcgc                                                                                                                                                                                    <  1:426301/195‑1 (MQ=255)                                                                                                                              gAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATg                                                                                                                       >  1:402540/1‑250 (MQ=255)                                                                                                                                  cTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAAt                                                                                                                                                               <  1:59404/208‑1 (MQ=255)                                                                                                                                    ggAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTAc                                                                                                                                                                                          >  1:369259/1‑179 (MQ=255)                                                                                                                                        aCGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGa                                                                                                            >  1:807067/1‑251 (MQ=255)                                                                                                                                                       aaaCTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑gcgc                                                                                                                                    <  1:187014/212‑1 (MQ=255)                                                                                                                                                         aCTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGTAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGataata                                                                                           <  1:140397/251‑1 (MQ=255)                                                                                                                                                          cTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAatat                                                                                          <  1:364819/251‑1 (MQ=255)                                                                                                                                                                    aTGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGAt                                                                                >  1:107041/1‑251 (MQ=255)                                                                                                                                                                           ccAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTg                                                                         <  1:766068/251‑1 (MQ=255)                                                                                                                                                                              aTTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCt                                                                      <  1:360320/251‑1 (MQ=255)                                                                                                                                                                                  cAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTaaaaa                                                                                                                                                     <  1:55249/170‑1 (MQ=255)                                                                                                                                                                                  cAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTaaaaa                                                                                                                                                     <  1:115130/170‑1 (MQ=255)                                                                                                                                                                                  cAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAatat                                                                                          <  1:892841/227‑1 (MQ=255)                                                                                                                                                                                  cAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAg                                                                  >  1:102439/1‑251 (MQ=255)                                                                                                                                                                                   aaGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 <  1:325191/251‑1 (MQ=255)                                                                                                                                                                                   aaGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 <  1:797619/251‑1 (MQ=255)                                                                                                                                                                                   aaGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 <  1:408635/251‑1 (MQ=255)                                                                                                                                                                                   aaGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 <  1:746459/251‑1 (MQ=255)                                                                                                                                                                                   aaGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 <  1:140759/251‑1 (MQ=255)                                                                                                                                                                                       aTTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAatat                                                                                          >  1:574730/1‑222 (MQ=255)                                                                                                                                                                                       aTTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCt                                                                      <  1:654752/242‑1 (MQ=255)                                                                                                                                                                                           aGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑gcgc                                                                                                                                    >  1:611122/1‑176 (MQ=255)                                                                                                                                                                                            gTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGATTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAAt                                                        >  1:410929/1‑251 (MQ=255)                                                                                                                                                                                                   gTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTa                                                 >  1:38833/1‑251 (MQ=255)                                                                                                                                                                                                        tCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTg                                            >  1:193144/1‑251 (MQ=255)                                                                                                                                                                                                         cccACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTgg                                           >  1:439994/1‑251 (MQ=255)                                                                                                                                                                                                              aaCCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTg                                      <  1:411256/251‑1 (MQ=255)                                                                                                                                                                                                               accttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAGACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTgg                                     >  1:390781/1‑251 (MQ=255)                                                                                                                                                                                                               accttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTcc                                                                                                     >  1:861985/1‑187 (MQ=255)                                                                                                                                                                                                               accttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAatat                                                                                          >  1:716618/1‑198 (MQ=255)                                                                                                                                                                                                               accttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATAttt                                                                                        >  1:647694/1‑200 (MQ=255)                                                                                                                                                                                                               accttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATAtt                                                                                         >  1:714298/1‑199 (MQ=255)                                                                                                                                                                                                               accttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTa                                                 >  1:528837/1‑239 (MQ=255)                                                                                                                                                                                                                 cttaccttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCa                                   <  1:793459/251‑1 (MQ=255)                                                                                                                                                                                                                    accttGCTTTTGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTc                                                                                                                                                                                                                              <  1:92100/63‑1 (MQ=255)                                                                                                                                                                                                                             ttGAAAACGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTAc                       <  1:891955/251‑1 (MQ=255)                                                                                                                                                                                                                                aaaaCGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGc                    <  1:667938/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGcaa                  >  1:669160/1‑250 (MQ=255)                                                                                                                                                                                                                                    cGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATAGCGCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGcaa                  >  1:468504/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGTTAAACATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTAATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 >  1:449595/1‑202 (MQ=255)                                                                                                                                                                                                                                        aaaCATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATgg                                                                                                                                                             <  1:112153/108‑1 (MQ=255)                                                                                                                                                                                                                                        aaaCATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATACTATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGCAACAATaa            >  1:455116/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCATAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGCAACAATaaaa          <  1:713903/251‑1 (MQ=255)                                                                                                                                                                                                                                             tAACCAAATTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCt                                                                      >  1:821873/1‑188 (MQ=255)                                                                                                                                                                                                                                                  aaaTTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAgc                                                                 <  1:674129/188‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaTTAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGCAACAATAAAATGTTTAAt  <  1:533385/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         AAATACAAGAACCAACAAAATGCCGTTGCCAGCCGCATTGATGAGGACAAATAAATGAAAAAGATCGCAATTATTACTGTGGTCGTCATACTTGCGATCTTTGCATTTTTTGATGTCCAAATTCAGAAACTGGAAACGGTGGTTTCCGCGAAACTGGCGCAACATGAAACCCAATTTCAAGTATTTAGTCTAGGGTTTTTCCCACAACCTTACCTTGCTTTTGAAAACGTTAAACATAACCAAATCAGCATTGAAAAACTGACCGGTAAATTTCCGTTATCCGCGTTGTTTTCTGGGAATGTCAAATTACAGGCGCTGGAAATCCAAAACGCCAAATGGTCTAAAAACGCGCAGAATA‑‑GCGCCGATATTCAAATGCGTTTTTCCGATTTCTCCCTTGATAATATTTCTTCCGATGGCATTGTCTTTAGCGGCGATAATTCAGTTACGGTGGAATTGGCAAAACCCTTGTACGGCAACAATAAAATGTTTAAT  >  1/986031‑986522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑