Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	981,779	T→C	intergenic (+31205/+24386)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	981,779	0	T	C	100.0%	213.5 / NA	74	intergenic (+31205/+24386)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/36);  total (38/36)

CCCTAATCCAACTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGATATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATACAGGATAAAATGAACTCTAT  >  1/981536‑982015                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             cccTAATCCAACTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGa                                                                                                                                                                                                                                       <  1:646421/251‑1 (MQ=255) cccTAATCCAACTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGa                                                                                                                                                                                                                                       <  1:782528/251‑1 (MQ=255) cccTAATCCAACTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGa                                                                                                                                                                                                                                       <  1:633032/251‑1 (MQ=255)    tAATCCAACTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCg                                                                                                                                                                                                                                    >  1:574063/1‑251 (MQ=255)            cTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGtt                                                                                                                                                                                                                            >  1:308215/1‑251 (MQ=255)                aaaTGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGAt                                                                                                                                                                                                                        <  1:832310/251‑1 (MQ=255)                         ggTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAg                                                                                                                                                                                                                                        <  1:80806/226‑1 (MQ=255)                         ggTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCCGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATaa                                                                                                                                                                                                               >  1:610833/1‑251 (MQ=255)                             aCAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCGTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATaaaaa                                                                                                                                                                                                            <  1:295530/250‑1 (MQ=255)                                  aGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAata                                                                                                                                                                                                      >  1:825505/1‑251 (MQ=255)                                  aGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAata                                                                                                                                                                                                      >  1:68600/1‑251 (MQ=255)                                  aGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAata                                                                                                                                                                                                      >  1:414875/1‑251 (MQ=255)                                  aGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAata                                                                                                                                                                                                      >  1:5325/1‑251 (MQ=255)                                   ggTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAatat                                                                                                                                                                                                     >  1:770825/1‑251 (MQ=255)                                      aaTATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTaa                                                                                                                                                                                                  >  1:576431/1‑251 (MQ=255)                                       atatAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAAt                                                                                                                                                                                                 <  1:719593/251‑1 (MQ=255)                                         ataGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAg                                                                                                                                                                                               >  1:59845/1‑251 (MQ=255)                                             agaATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGAc                                                                                                                                                                                            <  1:155793/250‑1 (MQ=255)                                                atatTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAAc                                                                                                                                                                                                                    >  1:657998/1‑223 (MQ=255)                                                atatTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAg                                                                                                                                                                                               >  1:602608/1‑244 (MQ=255)                                                 tatTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGGGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGc                                                                                                                                                                                       <  1:853727/251‑1 (MQ=255)                                                  atTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAgg                                                                                                                                                                                              >  1:540342/1‑243 (MQ=255)                                                       aCCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTa                                                                                                                                                                                 >  1:204325/1‑251 (MQ=255)                                                          tATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAag                                                                                                                                                                              >  1:529514/1‑251 (MQ=255)                                                           aTTACGGCACTACGTTTGGCAATGCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGa                                                                                                                                                                                                                                       <  1:429108/193‑1 (MQ=255)                                                                 gCACTACGTTTGGCAATTCTTGTTGTGAATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGAGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAAt                                                                                                                                                                                                 >  1:152972/1‑225 (MQ=255)                                                                      aCGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAg                                                                                                                                                                                                                                        >  1:577891/1‑181 (MQ=255)                                                                      aCGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAAtgatga                                                                                                                                                                  >  1:719910/1‑251 (MQ=255)                                                                                 ttCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGAc                                                                                                                                                                                            >  1:488609/1‑214 (MQ=255)                                                                                      gttgtGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCAt                                                                                                                                                  >  1:879370/1‑251 (MQ=255)                                                                                           gtATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGc                                                                                                                                                                                       <  1:39903/209‑1 (MQ=255)                                                                                                aCGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCAtt                                                                                                                                        <  1:378077/251‑1 (MQ=255)                                                                                                      atatCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCa                                                                                                                                  <  1:568024/251‑1 (MQ=255)                                                                                                             aCCAAATGCTGTAGCGAGTACGCCTACTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAAtt                                                                                                                                                                                  <  1:456461/196‑1 (MQ=255)                                                                                                                  aTGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGAc                                                                                                                                                                                            >  1:430582/1‑181 (MQ=255)                                                                                                                  aTGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGAc                                                                                                                                               <  1:401328/226‑1 (MQ=255)                                                                                                                                 cGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATaaaaa                                                                                                                                                                                                            >  1:322868/1‑150 (MQ=255)                                                                                                                                 cGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAAt                                                                                                                                                                                                 <  1:768511/161‑1 (MQ=255)                                                                                                                                 cGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATACTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGt                                                                                                       >  1:432489/1‑251 (MQ=255)                                                                                                                                   ccTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTg                                                                                                      >  1:417602/1‑250 (MQ=255)                                                                                                                                   ccTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTg                                                                                                      >  1:632593/1‑250 (MQ=255)                                                                                                                                   ccTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGc                                                                                                     <  1:291345/251‑1 (MQ=255)                                                                                                                                        tgtgCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATgg                                                                                                <  1:203133/251‑1 (MQ=255)                                                                                                                                        tgtgCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATgg                                                                                                <  1:156517/251‑1 (MQ=255)                                                                                                                                                          tGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAaatagaat                                                                              <  1:401366/251‑1 (MQ=255)                                                                                                                                                          tGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAaat                                                                                   <  1:217681/246‑1 (MQ=255)                                                                                                                                                           gAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAAtt                                                                                                                                                                                  <  1:850007/150‑1 (MQ=255)                                                                                                                                                                    atgatTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACt                                                                    <  1:154764/251‑1 (MQ=255)                                                                                                                                                                              caacaTAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCa                                                          >  1:470125/1‑251 (MQ=255)                                                                                                                                                                                acaTAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTaa                                                                                                                                                                                >  1:387737/1‑131 (MQ=255)                                                                                                                                                                                acaTAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATgg                                                                                                <  1:363236/211‑1 (MQ=255)                                                                                                                                                                                acaTAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATgg                                                                                                <  1:680974/211‑1 (MQ=255)                                                                                                                                                                                acaTAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAAt                                                        >  1:427902/1‑251 (MQ=255)                                                                                                                                                                                        ctgctTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGAtt                                                                                                          >  1:106936/1‑193 (MQ=255)                                                                                                                                                                                          gctTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGAt                                                                                                                                                                                                                                      <  1:564561/67‑1 (MQ=255)                                                                                                                                                                                          gctTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAaatagaat                                                                              <  1:745707/219‑1 (MQ=255)                                                                                                                                                                                          gctTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTgag                                              >  1:547810/1‑251 (MQ=255)                                                                                                                                                                                             ttATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATgg                                                                                                <  1:682223/198‑1 (MQ=255)                                                                                                                                                                                               ataataTCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTaa                                                                                                                                                                                <  1:839227/116‑1 (MQ=255)                                                                                                                                                                                                    atCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAAt                                                                                                                                                                                                 <  1:614022/94‑1 (MQ=255)                                                                                                                                                                                                    atCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGc                                                                                                                                                                                       <  1:672576/104‑1 (MQ=255)                                                                                                                                                                                                    atCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAaat                                                                                   >  1:802999/1‑204 (MQ=255)                                                                                                                                                                                                    atCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGAc                                            <  1:436845/243‑1 (MQ=255)                                                                                                                                                                                                     tCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGACTAGTGCTATGGTGAGAAc                                                                                         >  1:811904/1‑197 (MQ=255)                                                                                                                                                                                                                    ggAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATAc                     <  1:815667/250‑1 (MQ=255)                                                                                                                                                                                                                    ggAACAAAATCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATAc                     <  1:24961/250‑1 (MQ=255)                                                                                                                                                                                                                          aaaTCTGTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAAtt                                                                                                                                                                                  >  1:102786/1‑87 (MQ=255)                                                                                                                                                                                                                                gTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCAtt                                                                                                                                        <  1:858506/123‑1 (MQ=255)                                                                                                                                                                                                                                gTCCCTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATACAGGATAAAATGaa        >  1:844769/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTg                                                >  1:166191/1‑208 (MQ=255)                                                                                                                                                                                                                                   ccTTTATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATACAGGATAAAATGAAct      >  1:595738/1‑250 (MQ=255)                                                                                                                                                                                                                                      ttATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGAc                                                                                                                                                                                            <  1:283034/65‑1 (MQ=255)                                                                                                                                                                                                                                      ttATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATACAGGATAAAATGAACTCTAt  >  1:493824/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttATAGAACATTGACATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATACAGGATAAAATGAACTCTAt  >  1:640852/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             CCCTAATCCAACTACAAATGGTGGGGTGACAATAGGTAATATAGAGAATATTTTACCTATTACGGCACTACGTTTGGCAATTCTTGTTGTGTATAACGCGAATATCAAACCAAATGCTGTAGCGAGTACGCCTATTGTGCCGGCAACAGACAGTGAGTTTAGGATGATTCTGACAACATAAGGCTGCTTTATAATATCTATGACTTGCCCGGGAACAAAATCTGTCCCTTTATAGAACATTGATATAAAGATCGCCAATGTTGGATAAACGATAAAAAAGAATATTAATAGGACAATGCTAATTAAAGAGCCAATGATGAATTTATCACTTTGCATGACTTTCATTTTGGCAAGTGCATTCGTAGAAACTCCGATTAGTGCTATGGTGAGAACAAAAATAGAATAGCCCAAACTGATTTTTGCAATTGTGGCTGAGACCATCACAAATCCAAAAATTATACAGGATAAAATGAACTCTAT  >  1/981536‑982015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑