Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	978,939	A→G	intergenic (+28365/+27226)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	978,939	0	A	G	100.0%	123.0 / NA	44	intergenic (+28365/+27226)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/26);  total (18/26)

AAAAATTGTTATTCGTTTCGGTTACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGACAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCATTTTTTAACAGGAAAACCCCTTGTTCGGAAA  >  1/978692‑979176                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aaaaaTTGTTATTCGTTTCGGTTACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAg                                                                                                                                                                                                                                            <  1:840896/251‑1 (MQ=255) aaaaaTTGTTATTCGTTTCGGTTACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAg                                                                                                                                                                                                                                            <  1:494199/251‑1 (MQ=255)                   cGGTTACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTaaa                                                                                                                                                                                                                          <  1:800187/251‑1 (MQ=255)                     gTTACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAAt                                                                                                                                                                                                                         <  1:681392/250‑1 (MQ=255)                      ttACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATg                                                                                                                                                                                                                        <  1:302815/250‑1 (MQ=255)                                   aaGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAAt                                                                                                                                                                                                           <  1:805544/250‑1 (MQ=255)                                   aaGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATc                                                                                                                                                                                                          <  1:326804/251‑1 (MQ=255)                                                   gAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAAc                                                                                                                                                                                                                                  <  1:615273/211‑1 (MQ=255)                                                     aTAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTc                                                                                                                                                                                        <  1:122341/251‑1 (MQ=255)                                                               ttttGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATgg                                                                                                                                                                              <  1:396525/251‑1 (MQ=255)                                                                      gCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCg                                                                                                                                                                         >  1:426443/1‑249 (MQ=255)                                                                         ccTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGc                                                                                                                                                                                      <  1:668092/233‑1 (MQ=255)                                                                                  cTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAAc                                                                                                                                                                                                                                  >  1:114304/1‑180 (MQ=255)                                                                                            ggTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAAc                                                                                                                                                                                                                                  <  1:562613/170‑1 (MQ=255)                                                                                                    tttGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGAttt                                                                                                                                                                                            <  1:541713/200‑1 (MQ=255)                                                                                                    tttGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTAt                                                                                                                                                                                          <  1:29954/202‑1 (MQ=255)                                                                                                       gtgCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGCTTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGtttttt                                                                                                                                      >  1:439817/1‑251 (MQ=255)                                                                                                               cTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCg                                                                                                                               >  1:121220/1‑250 (MQ=255)                                                                                                                       aGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGAttt                                                                                                                                                                                            >  1:183954/1‑181 (MQ=255)                                                                                                                        gCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTTCCCGCACTTAAgg                                                                                                                      >  1:152375/1‑250 (MQ=255)                                                                                                                         cAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGc                                                                                                                     <  1:681285/250‑1 (MQ=255)                                                                                                                         cAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCt                                                                                                                    <  1:200223/251‑1 (MQ=255)                                                                                                                         cAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCt                                                                                                                    <  1:892070/251‑1 (MQ=255)                                                                                                                                  tAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTc                                                                                                           <  1:160221/251‑1 (MQ=255)                                                                                                                                          aGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTaaa                                                                                                                                                                                                                          <  1:78053/132‑1 (MQ=255)                                                                                                                                           gtgtTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGTATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCt                                                                                                                    >  1:36208/1‑233 (MQ=255)                                                                                                                                            tgtTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAac                                                                                                   <  1:248380/249‑1 (MQ=255)                                                                                                                                                         tttcttttTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATc                                                                                    <  1:458022/251‑1 (MQ=255)                                                                                                                                                          ttcttttTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCt                                                                                   <  1:867843/251‑1 (MQ=255)                                                                                                                                                                                    aGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCt                                                                                                                    >  1:734026/1‑192 (MQ=255)                                                                                                                                                                                                                    cacCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCaaaaa                                                                                                                                                  <  1:251215/130‑1 (MQ=255)                                                                                                                                                                                                                    cacCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAATCGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTg                                                                               <  1:295308/197‑1 (MQ=255)                                                                                                                                                                                                                    cacCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAg                                                                      >  1:773640/1‑206 (MQ=255)                                                                                                                                                                                                                    cacCTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCATTTTTTaa                         >  1:772410/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAAt                                                          >  1:455819/1‑216 (MQ=255)                                                                                                                                                                                                                      ccTATACCCAACCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCATTTTTTAACa                       >  1:896299/1‑251 (MQ=255)                                                                                                                                                                                                                                aCCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGc                                                                                                                     >  1:793145/1‑147 (MQ=255)                                                                                                                                                                                                                                aCCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATc                                                                                    <  1:433637/180‑1 (MQ=255)                                                                                                                                                                                                                                aCCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCt                                                                                   >  1:742836/1‑181 (MQ=255)                                                                                                                                                                                                                                aCCATAGGTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAAt                                                                            >  1:243815/1‑188 (MQ=255)                                                                                                                                                                                                                                      ggTAATTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCATTTTTTAAc                        >  1:410623/1‑234 (MQ=255)                                                                                                                                                                                                                                          aTTTATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCAtttt                             <  1:804936/225‑1 (MQ=255)                                                                                                                                                                                                                                           tttATTACCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCATTATTTAACAGGAAAACCCCTTGTTCGGaaa  >  1:762060/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aCCTATGGCAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAgg                                                                                                                      >  1:64119/1‑129 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AAAAATTGTTATTCGTTTCGGTTACATTGAGGCGAAGTTGATTATCCAAGGAATAAGGGAATTTTTGTAGCACCTGCTCCACTTGTTGATTGGTGTAAATTTGTGCACCGCTACCGATAGCAACGATGATAGCGGCAAGTGTTAACGTGACTTTTCTTTTTTTGCTCATGTTGTCCTCAAGTTTGAATTTTATCGGTTCCCCAAAGGGGAACACCTATACCCAACCATAGGTAATTTATTACCTATGACAGTTTCTAAAACGTCGTAAATGATTACTCATAAATCACAAAAAGTGATTTATTCGCCCCTATGGGGCCGCTGGCAGAGCCAACGTTCAAAAAACGTTGTTTTTTGTGCCCGCACTTAAGGCTTGTGTGCTCATTAAAACACACAAGCCCTAATCTTTTGAATACAAAGTGCGGTTAAAATTTCAATTATTTTTTAATTTTATTCATTTTTTAACAGGAAAACCCCTTGTTCGGAAA  >  1/978692‑979176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑