Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	960,553	T→G	intergenic (+9979/+45612)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	960,553	0	T	G	100.0%	138.1 / NA	51	intergenic (+9979/+45612)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/23);  total (28/23)

TGGCGTAGTTTCTTCTTTCCCCTCTTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTT‑‑CCC‑CCCC‑CTCAGGGCTTCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCGTGTTTGCCTTTGCACCCTTTTATTATTGGGG  >  1/960315‑960796                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    tgGCGTAGTTTCTTCTTTCCCCTCTTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTc                                                                                                                                                                                                                                           <  1:480671/250‑1 (MQ=255)    cGTAGTTTCTTCTTTCCCCTCTTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTccccc                                                                                                                                                                                                                                       <  1:882293/251‑1 (MQ=255)             ttcttTCCCCTCTTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGa                                                                                                                                                                                                                              <  1:590203/251‑1 (MQ=255)                   ccccTCTTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAg                                                                                                                                                                                                                        >  1:746834/1‑251 (MQ=255)                        ctTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAggg                                                                                                                                                                                                                           <  1:516605/243‑1 (MQ=255)                        ctTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAg                                                                                                                                                                                                                   >  1:288663/1‑251 (MQ=255)                        ctTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAAGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGGGCCAAg                                                                                                                                                                                                                   >  1:754041/1‑251 (MQ=255)                             gAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGttt                                                                                                                                                                                                                >  1:730824/1‑249 (MQ=255)                                                gagTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAagt                                                                                                                                                                                             <  1:63233/249‑1 (MQ=255)                                                gagTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAag                                                                                                                                                                                              >  1:657752/1‑248 (MQ=255)                                                     gAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTAc                                                                                                                                                                                       <  1:52326/250‑1 (MQ=255)                                                      aaCGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACaa                                                                                                                                                                                     <  1:553490/251‑1 (MQ=255)                                                        cGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATg                                                                                                                                                                                   <  1:81856/251‑1 (MQ=255)                                                        cGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATg                                                                                                                                                                                   <  1:41699/251‑1 (MQ=255)                                                        cGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTCCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAAc                                                                                                                                                                                                                                <  1:425517/206‑1 (MQ=255)                                                                          tctcCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGtt                                                                                                                                                                 <  1:876185/251‑1 (MQ=255)                                                                          tctcCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGtt                                                                                                                                                                 >  1:314580/1‑251 (MQ=255)                                                                             cccGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTc                                                                                                                                                               >  1:351018/1‑250 (MQ=255)                                                                                     caGCTGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTAc                                                                                                                                                                                       <  1:729028/218‑1 (MQ=255)                                                                                               aaaCATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAg                                                                                                                                                                         >  1:570037/1‑222 (MQ=255)                                                                                                  cATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTGCGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTT‑‑CCCTCCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAAc                                                                                                                                                                           <  1:40340/217‑1 (MQ=255)                                                                                                  cATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAagt                                                                                                                                                                                             <  1:803778/199‑1 (MQ=255)                                                                                                  cATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACGGCTGTGGtcgggaga                                                                                                                                                           >  1:894417/1‑226 (MQ=255)                                                                                                   aTTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACGGCTGTGGtcgggag                                                                                                                                                            <  1:149800/231‑7 (MQ=255)                                                                                                    ttATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGcc                                                                                                                                                                                                                      >  1:802416/1‑172 (MQ=255)                                                                                                    ttATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAagta                                                                                                                                                                                            >  1:309034/1‑198 (MQ=255)                                                                                                      ataATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGtt                                                                                                                                                                 >  1:624899/1‑223 (MQ=255)                                                                                                      ataATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGtt                                                                                                                                                                 >  1:497779/1‑223 (MQ=255)                                                                                                        aaTTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGtt                                                                                                                                                                 >  1:140671/1‑221 (MQ=255)                                                                                                                   atTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACGGCTGTGGtcgggag                                                                                                                                                            >  1:805056/1‑209 (MQ=255)                                                                                                                     ttttAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTc                                                                                                                                                                                                          >  1:387442/1‑167 (MQ=255)                                                                                                                                  gCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACaa                                                                                                                                                                                     >  1:170041/1‑175 (MQ=255)                                                                                                                                  gCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTgaga                                                                                                         >  1:3273/1‑251 (MQ=255)                                                                                                                                                         gTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAAt                                                                                                                  <  1:500474/219‑1 (MQ=255)                                                                                                                                                         gTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTT‑‑CCC‑CCCCGCTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATg                                                                                                                                                                                   <  1:233502/154‑1 (MQ=255)                                                                                                                                                                              ggATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCActtatct                                                              <  1:242776/250‑1 (MQ=255)                                                                                                                                                                               gATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTCCCCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAata                                                                          >  1:207560/1‑238 (MQ=255)                                                                                                                                                                                    aTCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAAt                                                                                                                  >  1:459356/1‑192 (MQ=255)                                                                                                                                                                                    aTCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACgcgcgc                                                                                               >  1:20837/1‑211 (MQ=255)                                                                                                                                                                                                           tGCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCgt                                >  1:626301/1‑251 (MQ=255)                                                                                                                                                                                                            gCTTAAAAATTGAGATTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGc                                                                                                                                                    >  1:791368/1‑134 (MQ=255)                                                                                                                                                                                                                       gagaTTTTC‑CCC‑CCCC‑CTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCActtatctt                                                             >  1:219079/1‑210 (MQ=255)                                                                                                                                                                                                                                      ccc‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACaa                                                                                                                                                                                     <  1:48814/77‑1 (MQ=255)                                                                                                                                                                                                                                       cc‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTc                                                                                                                                          <  1:128512/119‑1 (MQ=255)                                                                                                                                                                                                                                        c‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGtt                                                                                                                                                                 >  1:627409/1‑95 (MQ=255)                                                                                                                                                                                                                                        c‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGc                                                                                       <  1:401754/169‑1 (MQ=255)                                                                                                                                                                                                                                        c‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCActtatct                                                              >  1:392493/1‑194 (MQ=255)                                                                                                                                                                                                                                        c‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCGTGTTTGCCTTTGCACCCTTttatta        <  1:250302/248‑1 (MQ=255)                                                                                                                                                                                                                                        c‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCGTGTTTGCCTTTGCACCCTTttatta        <  1:667157/248‑1 (MQ=255)                                                                                                                                                                                                                                        c‑cTCAGGGCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCGTGTTTGCCTTTGCACCCTTTTATTATTg     >  1:770048/1‑251 (MQ=255)                                                                                                                                                                                                                                              gggCTGCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCGTGTTTTCCTTTGCACCCTTTTATTATTtggg  >  1:324663/1‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    TGGCGTAGTTTCTTCTTTCCCCTCTTTAGAAAAGAGGGGCGGGGGGAGAGTTGAACGATAACGGTGGCGCGCGTCTCCCGACCACAGCCGTTCGAAACATTATAATTCATTGATATTTTTAAATATCTTGCCTTCCTCCGTTTACGGGCAAGGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATTTTTTCACAAGATCATGCTTAAAAATTGAGATTTT‑‑CCC‑CCCC‑CTCAGGGCTTCGCCCAGCTCCCCCTGTGAACGAGGGGAGCCAAGTTTTTTTTCTTTTTATATAAGTAGTTACAATGTTTCGAACAGCTGTGGTTTCCCGACGCGTGCCGTTTATTTCATTAAATAACGAAAATTTAGGAATTGCGTGAGAAAACGCGCGCTATCCCGCTTATGAACAAATATTTCACTTATCTTATCGCACTCATTTTCGGCGCTGCCGGCGTGTTTGCCTTTGCACCCTTTTATTATTGGGG  >  1/960315‑960796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑