Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 952,307 G→A intergenic (+1733/+53858) unknown → / ← unknown tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1952,3070GA100.0% 203.0 / NA 72intergenic (+1733/+53858)unknown/unknowntRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/38);  total (34/38)

TAAAGCCCAGATAAAAAGAGGCAACACCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAGTCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGACGATTAAGCTGCCGAAGGCAATGATGGCGCCGGCGGTCCACCAGGATTTGATGTCGTTA  >  1/952062‑952542
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           
tAAAGCCCAGATAAAAAGAGGCAACACCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCaaa                                                                                                                                                                                                                                        <  1:288510/251‑1 (MQ=255)
         gATAAAAAGAGGCAACACCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATaaaaaa                                                                                                                                                                                                                               >  1:721523/1‑251 (MQ=255)
            aaaaaGAGGCAACACCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGc                                                                                                                                                                                                                            <  1:680422/251‑1 (MQ=255)
                    gCAACACCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACa                                                                                                                                                                                                                     <  1:184623/250‑1 (MQ=255)
                         acCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCa                                                                                                                                                                                                               >  1:280843/1‑251 (MQ=255)
                          cccAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCGTTCATGTTTTGCTCTTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACa                                                                                                                                                                                                                     <  1:441568/244‑1 (MQ=255)
                          cccAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGc                                                                                                                                                                                                                            >  1:411017/1‑237 (MQ=255)
                          cccAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCat                                                                                                                                                                                                              >  1:278761/1‑251 (MQ=255)
                            cAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatat                                                                                                                                                                                                            <  1:638366/251‑1 (MQ=255)
                                aCCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:792521/251‑1 (MQ=255)
                                aCCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:753813/251‑1 (MQ=255)
                                aCCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:643558/251‑1 (MQ=255)
                                aCCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:22081/251‑1 (MQ=255)
                                aCCGTTGCAATAGAAAGCGCAGATAAGCCTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:641700/251‑1 (MQ=255)
                                      gCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatat                                                                                                                                                                                                            >  1:371450/1‑241 (MQ=255)
                                      gCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTa                                                                                                                                                                                                  >  1:410474/1‑251 (MQ=255)
                                          tAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAAtt                                                                                                                                                                                              <  1:683895/251‑1 (MQ=255)
                                              aaGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATc                                                                                                                                                                                          <  1:396333/251‑1 (MQ=255)
                                                  gcAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTa                                                                                                                                                                                      >  1:111125/1‑251 (MQ=255)
                                                   cAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAAc                                                                                                                                                                                                                      >  1:618734/1‑218 (MQ=255)
                                                   cAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatat                                                                                                                                                                                                            >  1:131772/1‑228 (MQ=255)
                                                     gATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTa                                                                                                                                                                                   >  1:833293/1‑251 (MQ=255)
                                                        aaGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAAc                                                                                                                                                                                <  1:497509/251‑1 (MQ=255)
                                                           cTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACg                                                                                                                                                                               <  1:368141/249‑1 (MQ=255)
                                                            ttAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAatat                                                                                                                                                                                                                                     <  1:797573/194‑1 (MQ=255)
                                                            ttAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACa                                                                                                                                                                                                                     >  1:721293/1‑210 (MQ=255)
                                                             tAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCAC‑TAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATAtt                                                                                                                                                                          >  1:464360/1‑251 (MQ=255)
                                                               gTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatat                                                                                                                                                                                                            >  1:315361/1‑216 (MQ=255)
                                                               gTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:130952/220‑1 (MQ=255)
                                                                    ccATTGAATATTTTTTCATTCATGTTTTGCTTCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAAc                                                                                                                                                                                                                      <  1:428679/201‑1 (MQ=255)
                                                                      aTTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAAc                                                                                                                                                                                                                      >  1:852863/1‑199 (MQ=255)
                                                                           atatTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGT‑ACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAg                                                                                                                                                            <  1:21599/251‑1 (MQ=255)
                                                                               tttttCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGa                                                                                                                                                         <  1:286890/251‑1 (MQ=255)
                                                                                      ttcatGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTACTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACa                                                                                                                                                                                                                     <  1:478583/184‑1 (MQ=255)
                                                                                         atGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATaa                                                                                                                                                                                                                                   >  1:814239/1‑167 (MQ=255)
                                                                                           gTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatatat                                                                                                                                                                                                          >  1:15514/1‑190 (MQ=255)
                                                                                           gTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCAt                                                                                                                                             >  1:242632/1‑251 (MQ=255)
                                                                                                gCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAAtt                                                                                                                                                                                              >  1:376181/1‑197 (MQ=255)
                                                                                                  tCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatat                                                                                                                                                                                                            <  1:531560/181‑1 (MQ=255)
                                                                                                  tCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATc                                                                                                                                               <  1:639503/242‑1 (MQ=255)
                                                                                                      tACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        >  1:714770/1‑181 (MQ=255)
                                                                                                            atcaatAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACAt                                                                                                                                                                                                                    >  1:191364/1‑163 (MQ=255)
                                                                                                            atcaatAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCatatat                                                                                                                                                                                                          <  1:212687/173‑1 (MQ=255)
                                                                                                            atcaatAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:557751/175‑1 (MQ=255)
                                                                                                                atAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        >  1:176754/1‑171 (MQ=255)
                                                                                                                   ggTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACa                                                                                                                                                                                                                     >  1:445657/1‑155 (MQ=255)
                                                                                                                   ggTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:656895/168‑1 (MQ=255)
                                                                                                                          cccGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTaa                                                                                                                                                                                                                       <  1:464141/146‑1 (MQ=255)
                                                                                                                              gaagaaTCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        >  1:781912/1‑157 (MQ=255)
                                                                                                                                 gaaTCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTg                                                                                                                                                                                        <  1:764701/170‑1 (MQ=255)
                                                                                                                                                  gCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAAc                                                                                                                                                                                <  1:732085/161‑1 (MQ=255)
                                                                                                                                                  gCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCcca                                                                                       >  1:18320/1‑250 (MQ=255)
                                                                                                                                                          gAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGtt                                                                                                                                      <  1:484232/195‑1 (MQ=255)
                                                                                                                                                             tACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAAc                                                                                                                 <  1:654722/213‑1 (MQ=255)
                                                                                                                                                                  aaaaTGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAat                                                                                                                                                                                                                                       <  1:692561/90‑1 (MQ=255)
                                                                                                                                                                          cTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAAc                                                                                                                                                                                                                      >  1:1314/1‑99 (MQ=255)
                                                                                                                                                                          cTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTatat                                                                                                                             >  1:506644/1‑188 (MQ=255)
                                                                                                                                                                            tAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATAc                                                                                                                                                                                                        <  1:107839/111‑1 (MQ=255)
                                                                                                                                                                            tAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGtt                                                                                                                                                                  >  1:369656/1‑149 (MQ=255)
                                                                                                                                                                            tAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTc                                                                                                                        >  1:616833/1‑191 (MQ=255)
                                                                                                                                                                            tAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGa                                                             >  1:275978/1‑250 (MQ=255)
                                                                                                                                                                            tAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGa                                                             >  1:675455/1‑250 (MQ=255)
                                                                                                                                                                                  gACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAAt                                                                                                                                                <  1:510538/161‑1 (MQ=255)
                                                                                                                                                                                       cGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAg                                                                 <  1:182674/235‑1 (MQ=255)
                                                                                                                                                                                         cATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGc                                                                                                                                                                      >  1:702133/1‑132 (MQ=255)
                                                                                                                                                                                               aTCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAAt                                                                                                                                                                                               <  1:68895/101‑1 (MQ=255)
                                                                                                                                                                                               aTCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCAttt                                                                                                                                           <  1:6972/153‑1 (MQ=255)
                                                                                                                                                                                                           aTTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGACGATTAAGCTg                                                  <  1:618799/230‑1 (MQ=255)
                                                                                                                                                                                                                    ccTTTGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAAc                                                                                                                                                                                <  1:74648/95‑1 (MQ=255)
                                                                                                                                                                                                                      tttGGGCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGACGATTAAGCTGCCGAAGGCAATGATGGCGCCGGCGGTccacca                  >  1:478387/1‑251 (MQ=255)
                                                                                                                                                                                                                         gggCTTAGAAAAAATTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCccac                                                                                      >  1:348105/1‑180 (MQ=255)
                                                                                                                                                                                                                                      aTTAAATAAGCCGCAATCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGACGATTAAGCTGCCGAAGGCAATGATGGCGCCGGCGGTCCACCAGGATTTGATGTCGTta  >  1:21963/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           
TAAAGCCCAGATAAAAAGAGGCAACACCCAAGACCGTTGCAATAGAAAGCGCAGATAAGCTTAGTATTCCATTGAATATTTTTTCATTCATGTTTTGCTCCTTACTCAATCAATAGGTAACACCCGGAAGAATCATTGTTTTCGTCGCCATAAAGAATACAAAAAATGCACTTAATACGACTACGCATGTAATCCTATAGGCTATTTTTTCTCCTTTGGGCTTAGAAAAAATTAAATAAGCCGCAGTCAAATATAAAAAAAGCTGTAACATTTCCATATATACTCCTTAAATTTATCTGTATTAAACGATATTTAGCGGTTAGTAAGTGAAGATAGAATCATTTTAGTTATTCTATATTTTTCTTACAACTAAGCTAAATTGAACAGGAATTCCCACCACGCCATACCGATAGTTAAGTGGACGATTAAGCTGCCGAAGGCAATGATGGCGCCGGCGGTCCACCAGGATTTGATGTCGTTA  >  1/952062‑952542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑