Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	944,205	T→C	intergenic (+21902/‑6283)	unknown → / → unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	944,205	0	T	C	100.0%	202.2 / NA	73	intergenic (+21902/‑6283)	unknown/unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/40);  total (33/40)

GATTTTAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGTCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGTCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTCTTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAATGTGAACGGACG  >  1/943962‑944430                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  gATTTTAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGt                                                                                                                                                                                                                            >  1:818820/1‑251 (MQ=255)  aTTTTAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGtt                                                                                                                                                                                                                           >  1:656632/1‑251 (MQ=255)  aTTTTAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGt                                                                                                                                                                                                                            <  1:851045/250‑1 (MQ=255)  aTTTTAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGt                                                                                                                                                                                                                            <  1:29694/250‑1 (MQ=255)      tAAACAGATGTTCCACTTCTTTTTCGGAAAGTGAGGATGCCGTTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCAATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCAAAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATcc                                                                                                                                                                                                                       <  1:779549/251‑1 (MQ=255)      tAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATcc                                                                                                                                                                                                                       <  1:415124/251‑1 (MQ=255)      tAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATcc                                                                                                                                                                                                                       <  1:579223/251‑1 (MQ=255)       aaaCAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCa                                                                                                                                                                                                                      <  1:665959/251‑1 (MQ=255)            gATGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACg                                                                                                                                                                                                                 >  1:640293/1‑251 (MQ=255)             aTGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACg                                                                                                                                                                                                                 >  1:586864/1‑250 (MQ=255)             aTGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGc                                                                                                                                                                                                                <  1:469031/251‑1 (MQ=255)             aTGTTCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGc                                                                                                                                                                                                                <  1:842464/251‑1 (MQ=255)                ttCCACTTCTTTTTCGGAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCt                                                                                                                                                                                                               <  1:204504/249‑1 (MQ=255)                                gAAAGGGAGGATGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGAtt                                                                                                                                                                                             >  1:197154/1‑251 (MQ=255)                                     ggaggaTGCCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAAtt                                                                                                                                                                                        >  1:184227/1‑251 (MQ=255)                                          atgccGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTgg                                                                                                                                                                                   <  1:846545/247‑1 (MQ=255)                                               ggTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCa                                                                                                                                                                               >  1:273792/1‑250 (MQ=255)                                                     tCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGa                                                                                                                                                                        >  1:464111/1‑251 (MQ=255)                                                     tCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGa                                                                                                                                                                        >  1:708713/1‑251 (MQ=255)                                                           aTCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGc                                                                                                                                                                   >  1:232622/1‑250 (MQ=255)                                                                 aTTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGc                                                                                                                                                                                                                <  1:4568/199‑1 (MQ=255)                                                                        cGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAACCGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACTATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGc                                                                                                                                                      <  1:802731/250‑1 (MQ=255)                                                                        cGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGc                                                                                                                                                      <  1:676797/250‑1 (MQ=255)                                                                             taAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTAttt                                                                                                                                                <  1:446378/251‑1 (MQ=255)                                                                               aGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGc                                                                                                                                                                                <  1:84024/217‑1 (MQ=255)                                                                                  aaaCGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAgg                                                                                                                                           <  1:102336/251‑1 (MQ=255)                                                                                          tCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAg                                                                                                                                   >  1:739975/1‑251 (MQ=255)                                                                                               aTTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTaa                                                                                                                               >  1:764932/1‑250 (MQ=255)                                                                                                  tCGATCATCTGCATTTGAGAAACGGAAATTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAATTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACggg                                                                                                                           <  1:468746/251‑1 (MQ=255)                                                                                                  tCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGc                                                                                                                                                      >  1:67481/1‑224 (MQ=255)                                                                                                       catcTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACggg                                                                                                                           <  1:57227/246‑1 (MQ=255)                                                                                                        atcTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGA‑TTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCt                                                                                                                    <  1:374341/251‑1 (MQ=255)                                                                                                            gCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCAt                                                                                                                                                                                                                     <  1:828499/151‑1 (MQ=255)                                                                                                            gCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACg                                                                                                                                                                                                      >  1:859679/1‑166 (MQ=255)                                                                                                            gCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTaaa                                                                                                                 >  1:614891/1‑251 (MQ=255)                                                                                                                              gTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACAATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCAc                                                                                                                                                                              >  1:39035/1‑172 (MQ=255)                                                                                                                                             ctttcGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATa                                                                                >  1:832760/1‑251 (MQ=255)                                                                                                                                                   gATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAatat                                                                                                       >  1:210922/1‑222 (MQ=255)                                                                                                                                                   gATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCttt                                                                                          <  1:747702/235‑1 (MQ=255)                                                                                                                                                   gATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAAt                                                                          >  1:314412/1‑251 (MQ=255)                                                                                                                                                        atatcaatatcCAACTCGTCAAAAATGGCTTTGGTATCTCGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACa                                                                     <  1:461412/251‑1 (MQ=255)                                                                                                                                                           tcaatatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGCTAACGTCCGAGCCACAGGCTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCAAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGc                                                                                                                          >  1:95305/1‑195 (MQ=255)                                                                                                                                                           tcaatatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACAttt                                                                  >  1:89673/1‑251 (MQ=255)                                                                                                                                                              atatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGc                                                                                                                                                                   >  1:30161/1‑151 (MQ=255)                                                                                                                                                              atatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAg                                                                                                                                   >  1:592339/1‑183 (MQ=255)                                                                                                                                                              atatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGcc                                                                                                   >  1:346444/1‑215 (MQ=255)                                                                                                                                                              atatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACa                                                                     <  1:306176/245‑1 (MQ=255)                                                                                                                                                              atatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAg                                                               >  1:356777/1‑251 (MQ=255)                                                                                                                                                               tatcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGc                                                              <  1:687622/251‑1 (MQ=255)                                                                                                                                                                atcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAg                                                                                                                                            >  1:516032/1‑172 (MQ=255)                                                                                                                                                                atcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGC‑TATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAg                                                            <  1:183564/251‑1 (MQ=255)                                                                                                                                                                 tcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAg                                                            <  1:696233/251‑1 (MQ=255)                                                                                                                                                                 tcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAg                                                            <  1:673354/251‑1 (MQ=255)                                                                                                                                                                 tcCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAg                                                            <  1:439088/251‑1 (MQ=255)                                                                                                                                                                                  ggCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACggg                                                                                                                           >  1:459439/1‑171 (MQ=255)                                                                                                                                                                                  ggCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATAc                                                                               <  1:20855/215‑1 (MQ=255)                                                                                                                                                                                              aCGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGc                                                              <  1:487226/220‑1 (MQ=255)                                                                                                                                                                                               cGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTAcc                                                <  1:316916/233‑1 (MQ=255)                                                                                                                                                                                                 aTAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCa                            >  1:640440/1‑251 (MQ=255)                                                                                                                                                                                                      ttttGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCg                       <  1:428224/251‑1 (MQ=255)                                                                                                                                                                                                            cGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTAc                  <  1:320052/250‑1 (MQ=255)                                                                                                                                                                                                            cGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTAc                  <  1:91644/250‑1 (MQ=255)                                                                                                                                                                                                              tGATCCACAAACGGTCCTTTCAGCGGATACCGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAg                <  1:707778/250‑1 (MQ=255)                                                                                                                                                                                                              tGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAg                <  1:665889/250‑1 (MQ=255)                                                                                                                                                                                                                aTCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCa                               <  1:418156/233‑1 (MQ=255)                                                                                                                                                                                                                aTCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATg                         >  1:274963/1‑239 (MQ=255)                                                                                                                                                                                                                aTCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGaa              <  1:604217/250‑1 (MQ=255)                                                                                                                                                                                                                aTCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAAt             >  1:166306/1‑251 (MQ=255)                                                                                                                                                                                                                 tCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAAtg            >  1:245711/1‑251 (MQ=255)                                                                                                                                                                                                                     caAACGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAATGTGaa        <  1:266126/251‑1 (MQ=255)                                                                                                                                                                                                                         cGGTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAATGTGAacgga    <  1:247499/251‑1 (MQ=255)                                                                                                                                                                                                                           gTCCTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAATGTGAacggacg  >  1:10355/1‑251 (MQ=255)                                                                                                                                                                                                                             ccTTTCAGCGGATAACGTCCGAGCCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTATTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAATGTGaa        >  1:130934/1‑243 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  GATTTTAAACAGATGTTCCACTTCTTTTTCGGAAAGGGAGGATGTCGGTTCATCCATAATCACGATTTTGGCGTTATAAGAAAACGCTTTCGCAATTTCGATCATCTGCATTTGAGAAACGGAAAGTTTCGCCACTTTTTCTTTCGGATCAATATCAATATCCAACTCGTCAAAAATGGCTTTGGTATCACGATAGATTTTGGCGTGATCCACAAACGGTCCTTTCAGCGGATAACGTCCGAGTCACAGGTTATCCATTACGCTGGTCTGACGCACCAGATTCAATTCCTGGTGCACCATGGAAATGCCGTTTTCCAGGGCTTCTTTTGAGGTTTTAAAGTTAACGGGCTTGCCTAAAAATAAAATATCGCCTTCATCTTTAGCATAAATACCAAATAAACATTTTAGCAGTGTGGATTTACCCGCACCGTTTTCCCCCATCAATGCGTGTACAGAATGTGAACGGACG  >  1/943962‑944430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑