Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	922,977	T→G	intergenic (+674/‑27511)	unknown → / → unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	922,977	0	T	G	100.0%	96.2 / NA	35	intergenic (+674/‑27511)	unknown/unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/16);  total (19/16)

TTGAAACCACTTCTGACTTTTCAGATGTTGTTGGAAGAGTTGGAAAGTAGAATTGCCTATAGCAACACTAAGTTGTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGATCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAATGGCTTTACGTAACCAGCTTTG  >  1/922754‑923220                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    ttGAAACCACTTCTGACTTTTCAGATGTTGTTGGAAGAGTTGGAAAGTAGAATTGCCTATAGCAACACTAAGTTGTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAaataat                                                                                                                                                                                                                          <  1:793983/251‑1 (MQ=255)                      cAGATGTTGTTGGAAGAGTTGGAAAGTAGAATTGCCTATAGCTACACTAAGTTGTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGcggcgg                                                                                                                                                                                                      >  1:890124/1‑250 (MQ=255)                                     gagTTGGAAAGTAGAATTGCCTATAGCAACACTAAGTTGTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGt                                                                                                                                                                                                                <  1:354990/225‑1 (MQ=255)                                                                        gTTGTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAAt                                                                                                                                                   >  1:751997/1‑251 (MQ=255)                                                                           gTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCTGGGGGAATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATaaa                                                                                                                                                >  1:562530/1‑251 (MQ=255)                                                                                       aaTTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTtg                                                                                                                                       >  1:15064/1‑248 (MQ=255)                                                                                                        aTTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATg                                                                                                                   <  1:792229/251‑1 (MQ=255)                                                                                                                      atttTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATg                                                                                                                   >  1:702447/1‑237 (MQ=255)                                                                                                                                    aataaatGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAAt                                                                                       <  1:629195/251‑1 (MQ=255)                                                                                                                                      taaatGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     <  1:499840/251‑1 (MQ=255)                                                                                                                                        aatGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGcc                                                                                                                 >  1:278804/1‑221 (MQ=255)                                                                                                                                              aattaattGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTaa                                                                             <  1:366913/251‑1 (MQ=255)                                                                                                                                                 taattGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTaaaa                                                                           >  1:94709/1‑250 (MQ=255)                                                                                                                                                 taattGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTCTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     >  1:57581/1‑240 (MQ=255)                                                                                                                                                 taattGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTCGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     <  1:441817/240‑1 (MQ=255)                                                                                                                                                  aattGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATg                                                                         >  1:254501/1‑251 (MQ=255)                                                                                                                                                           tttCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTc                                                                                                     <  1:129105/214‑1 (MQ=255)                                                                                                                                                           tttCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     <  1:464234/230‑1 (MQ=255)                                                                                                                                                           tttCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATg                                                                         <  1:657077/242‑1 (MQ=255)                                                                                                                                                                aaaTAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAAcgc                                                                                                                                                                                                           <  1:159621/107‑1 (MQ=255)                                                                                                                                                                aaaTAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAg                                                                                                                                                                                               >  1:657283/1‑119 (MQ=255)                                                                                                                                                                aaaTAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAAt                                                                                       <  1:47420/223‑1 (MQ=255)                                                                                                                                                                     ggATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     <  1:93529/220‑1 (MQ=255)                                                                                                                                                                      gATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATa                                                                                      >  1:278299/1‑218 (MQ=255)                                                                                                                                                                           tCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTaaaa                                                                           >  1:579319/1‑224 (MQ=255)                                                                                                                                                                                        aTTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTtgctgc                                                                                                                                   >  1:798503/1‑155 (MQ=255)                                                                                                                                                                                             gCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGc                               <  1:293075/250‑1 (MQ=255)                                                                                                                                                                                               gTTGAATCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCa                              >  1:410980/1‑249 (MQ=255)                                                                                                                                                                                                    aTCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAAt                       >  1:891595/1‑251 (MQ=255)                                                                                                                                                                                                    aTCCAAATCTGTCTGCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAAt                       >  1:292351/1‑251 (MQ=255)                                                                                                                                                                                                              gtctgCGTTTGGTGTAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     <  1:360955/179‑1 (MQ=255)                                                                                                                                                                                                                         gtgtAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATac                                                                                     <  1:886807/168‑1 (MQ=255)                                                                                                                                                                                                                         gtgtAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAATGGCTTTACGTAACCAGCTTTg  >  1:729444/1‑251 (MQ=255)                                                                                                                                                                                                                         gtgtAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAATGGCTTTACGTAACCAGCTTTg  >  1:577236/1‑251 (MQ=255)                                                                                                                                                                                                                         gtgtAGAGCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAATGGCTTTACGTAACCAGCTTTg  >  1:270970/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    TTGAAACCACTTCTGACTTTTCAGATGTTGTTGGAAGAGTTGGAAAGTAGAATTGCCTATAGCAACACTAAGTTGTATTGGTTTTAAATTAATGGTGATTCCAATTTTAAGTCATTTATTTTAGATTTTAAAATAAATGAGAATTAATTGATATTTTCTAAATAGGATTTTCCCAGTTGATTCATTTGGCGTTGAATCCAAATCTGTCTGCGTTTGGTGTAGATCCCGGGGGCATTGACTTTGTAAATAATCGGATTGGGTAACGCGGCGGCTAAAAGTGCGGCTTCATTTTGCGATAAATTTTTTGCCGACTTTTTGAAATAAAAACGGCTTGCTGCTTCCACACCATAAATGCCATTCCCGAATTCGGCAATATTTAAATACACTTCTAAAATGCGTTTTTTTGACCACAAGGTTTCCAGCATCAAGGTCGTCGGCACTTCAATGGCTTTACGTAACCAGCTTTG  >  1/922754‑923220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑