Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	898,850	G→A	intergenic (‑49193/‑23368)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	898,850	0	G	A	100.0%	190.7 / NA	70	intergenic (‑49193/‑23368)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/42);  total (28/42)

AAACTGGTGAGTGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAGAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCATGATGACGCAGG  >  1/898614‑899096                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       aaaCTGGTGAGTGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAAccc                                                                                                                                                                                                                                          <  1:652352/251‑1 (MQ=255)        tGAGTGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGaaa                                                                                                                                                                                                                                    <  1:607355/250‑1 (MQ=255)        tGAGTGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGaaa                                                                                                                                                                                                                                    <  1:605438/250‑1 (MQ=255)        tGAGTGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGaaa                                                                                                                                                                                                                                    <  1:402302/250‑1 (MQ=255)            tGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGc                                                                                                                                                                                                                                 <  1:382305/249‑1 (MQ=255)                ttGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCtt                                                                                                                                                                                                                               >  1:14029/1‑247 (MQ=255)                ttGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTtgtg                                                                                                                                                                                                                           >  1:490569/1‑251 (MQ=255)                 tggtggACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTgg                                                                                                                                                                                                                          <  1:228053/251‑1 (MQ=255)                   gtggACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCtt                                                                                                                                                                                                                               <  1:176589/244‑1 (MQ=255)                          gCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATcgcg                                                                                                                                                                                                                 >  1:176950/1‑251 (MQ=255)                           ccTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATcgcg                                                                                                                                                                                                                 <  1:433706/250‑1 (MQ=255)                                aacaaATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGATGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACa                                                                                                                                                                                                           <  1:387924/251‑1 (MQ=255)                                     aTCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAg                                                                                                                                                                                                      <  1:512432/251‑1 (MQ=255)                                           ggCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATg                                                                                                                                                                                                                                                    <  1:525705/199‑1 (MQ=255)                                                     cGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCAGGGGCGCGAAAGTGGTGGAATTCGAACCGCTTAACGCCACCATCCATAAAATAAACGAGTGCGTGAGCGTGAAAGATCTTGGAAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTAAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATAcc                                                                                                                                                                                      <  1:737776/251‑1 (MQ=255)                                                         aTTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGaaa                                                                                                                                                                                                                                    >  1:752595/1‑201 (MQ=255)                                                                 ccGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCaa                                                                                                                                                                          >  1:510409/1‑251 (MQ=255)                                                                      ggtggCAGCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCAtt                                                                                                                                                                     >  1:97272/1‑251 (MQ=255)                                                                            cccTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTAc                                                                                                                                                               <  1:797209/250‑1 (MQ=255)                                                                               tCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAgg                                                                                                                                                            <  1:572206/251‑1 (MQ=255)                                                                                       acgTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTCATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCgcaggc                                                                                                                                                     <  1:840666/250‑1 (MQ=255)                                                                                          ttttATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGgcg                                                                                                                                                          >  1:811625/1‑242 (MQ=255)                                                                                           tttATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATg                                                                                                                                                                                                                                                    <  1:682722/151‑1 (MQ=255)                                                                                            ttATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATg                                                                                                                                                                                                                                                    <  1:362368/150‑1 (MQ=255)                                                                                                 gCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGc                                                                                                                                            >  1:615308/1‑249 (MQ=255)                                                                                                      cATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATAccc                                                                                                                                                                                     <  1:191394/203‑1 (MQ=255)                                                                                                      cATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCaa                                                                                                                                     >  1:499723/1‑251 (MQ=255)                                                                                                           gcgcGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCATGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCtt                                                                                                                                >  1:446267/1‑251 (MQ=255)                                                                                                                gAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAAt                                                                                                                                                                                                                     <  1:38715/161‑1 (MQ=255)                                                                                                                    tggtggAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAg                                                                                                                       <  1:539361/251‑1 (MQ=255)                                                                                                                          aaTTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGt                                                                                                                 >  1:300137/1‑251 (MQ=255)                                                                                                                           aTTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTg                                                                                                                     <  1:219424/246‑1 (MQ=255)                                                                                                                                    gCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATAccc                                                                                                                                                                                     <  1:131760/173‑1 (MQ=255)                                                                                                                                    gCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATgg                                                                                                        >  1:565772/1‑250 (MQ=255)                                                                                                                                                caTTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTa                                                                                                                                                                <  1:347747/182‑1 (MQ=255)                                                                                                                                                   tCATAAAGTAAACGAGTGCGTGGGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAAt                                                                                                          <  1:720051/233‑1 (MQ=255)                                                                                                                                                   tCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCg                                                                                        >  1:789953/1‑251 (MQ=255)                                                                                                                                                        aaGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAg                                                                                                                                                                                                      <  1:479059/136‑1 (MQ=255)                                                                                                                                                        aaGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATAAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAg                                                                                   <  1:803582/251‑1 (MQ=255)                                                                                                                                                           tAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAAGGCGGTCAGATTTACCATCAAAGGTTAGTGAATTTATTGGAAAATTAAAATGAGCTTACCCCCTGAAATGCTTTGTGGAAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGc                                                                                  <  1:687183/249‑1 (MQ=255)                                                                                                                                                              aCGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGttt                                                                             <  1:228542/251‑1 (MQ=255)                                                                                                                                                                 aGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCAc                                                                                                                                                                              <  1:872138/151‑1 (MQ=255)                                                                                                                                                                  gTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAAcc                                                                                                                                                                                                <  1:553040/132‑1 (MQ=255)                                                                                                                                                                            gTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCAcc                                                                                                                                                                             >  1:526044/1‑141 (MQ=255)                                                                                                                                                                             tGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAAc                                                                                                                                                                                                 <  1:287297/120‑1 (MQ=255)                                                                                                                                                                                aaGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGAAACATTAAAGGGAGCTTAACCCCTGAAATGCTTTGTGGCAAATGGCATGAACATTTGGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTc                                                            <  1:42398/250‑1 (MQ=255)                                                                                                                                                                                   aTCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTcagc                                                         <  1:807707/250‑1 (MQ=255)                                                                                                                                                                                        ggCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGa                                                   >  1:794273/1‑251 (MQ=255)                                                                                                                                                                                                cGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAAt                                           >  1:761492/1‑251 (MQ=255)                                                                                                                                                                                                  gTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCGCGTCAGCAGCTGATTTGGAATgg                                         >  1:103379/1‑251 (MQ=255)                                                                                                                                                                                                    cAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCa                                       >  1:412429/1‑251 (MQ=255)                                                                                                                                                                                                      gATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAAt                                                                                                 <  1:605918/191‑1 (MQ=255)                                                                                                                                                                                                      gATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCaaa                                     >  1:59751/1‑251 (MQ=255)                                                                                                                                                                                                      gATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCCTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGaa                                            >  1:76371/1‑244 (MQ=255)                                                                                                                                                                                                         ttACCATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAAttt                                  <  1:681775/251‑1 (MQ=255)                                                                                                                                                                                                             cATCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACg                              >  1:753696/1‑251 (MQ=255)                                                                                                                                                                                                              aTCAAATGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGc                             <  1:126221/251‑1 (MQ=255)                                                                                                                                                                                                                   aTGTTAGTGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGaa                         >  1:588755/1‑250 (MQ=255)                                                                                                                                                                                                                          tGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCGGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAg                  <  1:890722/250‑1 (MQ=255)                                                                                                                                                                                                                          tGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAg                  <  1:76367/250‑1 (MQ=255)                                                                                                                                                                                                                          tGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAg                  <  1:164103/250‑1 (MQ=255)                                                                                                                                                                                                                          tGAATTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAg                  <  1:874809/250‑1 (MQ=255)                                                                                                                                                                                                                            aaTTTATTGGAACATTAAAATGAGCTTACCCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTg                <  1:86381/250‑1 (MQ=255)                                                                                                                                                                                                                             aTTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTAc                                                                                                                                                               >  1:536488/1‑106 (MQ=255)                                                                                                                                                                                                                             aTTTATTGGAACATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCa              >  1:107803/1‑251 (MQ=255)                                                                                                                                                                                                                                        cATTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCATGATGACGCAg   >  1:484588/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGc               >  1:3270/1‑238 (MQ=255)                                                                                                                                                                                                                                         aTTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCATGATGACGc     <  1:253674/248‑1 (MQ=255)                                                                                                                                                                                                                                         aTTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCATGATGACGCAgg  >  1:716023/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTTAAAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAACAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCATGATGACGCAgg  >  1:456056/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       AAACTGGTGAGTGCGTTGGTGGACAGCCTGCAACAAATCGCAGGCATTACGCCGAAATTGGAAACCGGTGGTGGCACCTCAGACGGACGTTTTATTGCCCTCATGGGCGCGGAAGTGGTGGAATTCGGACCGCTTAACGCCACCATTCATAAAGTAAACGAGTGCGTGAGCGTGGAAGATCTTGGCAAATGCGGTCAGATTTACCATCAAATGTTAGTGAATTTATTGGAACATTAGAATGAGCTTAACCCCTGAAATGCTTTGTGGCAAATCGCGTGAACATTTAGTAAACCTGCCGATACCCCATTCACCCAATCATTTCTTACAGGCGCAGGCAGCGAAAGCCTTTCAAGGCTTACAAAAAAGTGCGGTAAAAAATGGCTTTAATTTACAGCCGGCGAGCAGTTTTCGTGATTTTGCACGTCAGCAGCTGATTTGGAATGGCAAATTTAACGCTGAACGTAAAGTGCATGATGACGCAGG  >  1/898614‑899096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑