Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	864,796	G→A	intergenic (‑15139/‑57422)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	864,796	0	G	A	100.0%	150.6 / NA	56	intergenic (‑15139/‑57422)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/24);  total (32/24)

GCGTTACGATGCCAACCTTGCCAAACAGATTCAGATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGAATCGGTTTATGGCTTGCTGACCTTGGCGCATTACCGAACCTCGCCGTTGGATTTACGTCGATTATTGGATGCGCCGCGACAACAATTTTATCTTGCGCAGACGCAGGAGGCCTTGTTGGGTTGTGTGTGGGCGGTGCCTGAGGGCAAATTTGGTGACGACGCATTAATTCGTAAAATTCGGCGCGGACAACGTCGTCCACGTGGTAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCACTGCGCATTTCCCGTATCGCCGTGCAACCCAACTGGCAGCAGCGGGGACTGGGGCAACGTTTAATCGAA‑CGAG‑TTAAACAACAACGTGCGGTGGATTTCCTTTCCGT  >  1/864583‑865029                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            gtgttatgacgctaaCCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGCTTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAAtt                                                                                                                                                                                                         >  1:890573/14‑250 (MQ=255) gtgttatgacgctaaCCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAAtt                                                                                                                                                                                                         >  1:283929/14‑250 (MQ=255)   gttatgacgctaaCCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCga                                                                                                                                                                                                      >  1:177839/12‑250 (MQ=255)                                   attTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtcgcctgcgcggcaatttgg                                                                                                                                                                       <  1:130677/250‑18 (MQ=255)                                   attTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtcgcctgcgcggcaatttgg                                                                                                                                                                       <  1:28453/250‑18 (MQ=255)                                           caaagaaCGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTc                                                                                                                                                                                                        >  1:803115/3‑209 (MQ=255)                                             aagaaCGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAAtg                                                                                                                                                            <  1:798858/251‑1 (MQ=255)                                                aacggATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAAtgttg                                                                                                                                                         <  1:809807/247‑1 (MQ=255)                                                  cggATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTtgtg                                                                                                                                                       <  1:390128/249‑1 (MQ=255)                                                                        aatcgGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtcgcctg                                                                                                                                                                                    >  1:114989/1‑196 (MQ=255)                                                                           cgGTTTATGGCTTGCTGACTTTGGCGCATTACCTCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGGCGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCg                                                                                                                              <  1:506287/251‑1 (MQ=255)                                                                            ggTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAAcgtc                                                                                                                                                                                         >  1:739850/1‑191 (MQ=255)                                                                             gTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAAc                                                                                                                                   <  1:882176/244‑1 (MQ=255)                                                                                tATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTaa                                                                                                                         <  1:733341/251‑1 (MQ=255)                                                                                        gCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAAtg                                                                                                                                                            >  1:390954/1‑208 (MQ=255)                                                                                                       ttacagcaCCTCGCCGTTGGATTTACGACGATTATTGGATGCACCGAGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGAGTGTGGGCGGAGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGGAAATTGGGTCCTTTGCGCATTTcc                                                                                                   >  1:210964/8‑250 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGAc                                                                                                                                                                                               <  1:97440/158‑1 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTtgtg                                                                                                                                                       <  1:701921/198‑1 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAAc                                                                                                                                   >  1:728742/1‑218 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc                                                                                                   >  1:209876/1‑250 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc                                                                                                   >  1:51847/1‑250 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc                                                                                                   <  1:582402/250‑1 (MQ=255)                                                                                                       ttACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTcc                                                                                                   >  1:698893/1‑250 (MQ=255)                                                                                                         aCCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTaa                                                                                                                         >  1:632915/1‑226 (MQ=255)                                                                                                                        tGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGccc                                                                                 <  1:158901/251‑1 (MQ=255)                                                                                                                                     attattGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCagcagc                                                                    <  1:69320/251‑1 (MQ=255)                                                                                                                                        attGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgag                                                                 >  1:232337/1‑249 (MQ=255)                                                                                                                                        attGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgag                                                                 >  1:483325/1‑249 (MQ=255)                                                                                                                                         ttGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGcagcag                                                                     >  1:789812/1‑246 (MQ=255)                                                                                                                                         ttGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCgagg                                                                >  1:781539/1‑248 (MQ=255)                                                                                                                                                caccgcGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTgggg                                                         <  1:759887/249‑1 (MQ=255)                                                                                                                                                    gcgACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTaa                                                                                                                         <  1:283615/183‑1 (MQ=255)                                                                                                                                                           caaTTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAAt                                              <  1:143521/251‑1 (MQ=255)                                                                                                                                                             attttattttGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGTCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGGATCGCCGTGc                                                                                      >  1:615681/1‑209 (MQ=255)                                                                                                                                                             attttattttGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCg                                            <  1:445071/251‑1 (MQ=255)                                                                                                                                                                  attttgcacaaacgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGAAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAAt                                              <  1:9417/220‑1 (MQ=255)                                                                                                                                                                            aacgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaac                           <  1:144890/237‑1 (MQ=255)                                                                                                                                                                              cgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAAt                                              >  1:790218/13‑232 (MQ=255)                                                                                                                                                                              cgcgggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaacaa                         <  1:585054/239‑1 (MQ=255)                                                                                                                                                                                  ggaaaccctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGt                      <  1:91252/242‑1 (MQ=255)                                                                                                                                                                                         ctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAAc                                                                                                                                   >  1:701442/2‑136 (MQ=255)                                                                                                                                                                                         ctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTggg              >  1:596187/2‑250 (MQ=255)                                                                                                                                                                                         ctgttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTggg              >  1:308059/2‑250 (MQ=255)                                                                                                                                                                                           gttgGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGAtt            >  1:386119/1‑251 (MQ=255)                                                                                                                                                                                                   gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTa                                                                                               >  1:833883/1‑162 (MQ=255)                                                                                                                                                                                                   gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑cagattaaac                               <  1:539036/225‑10 (MQ=255)                                                                                                                                                                                                   gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaacaa                         <  1:471552/230‑1 (MQ=255)                                                                                                                                                                                                   gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGt                      >  1:297852/1‑233 (MQ=255)                                                                                                                                                                                                   gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGAttttctttc     >  1:892622/1‑250 (MQ=255)                                                                                                                                                                                                   gtgtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGGGGAttttctttc     >  1:30271/1‑250 (MQ=255)                                                                                                                                                                                                     gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTTCCGTATCGCCGTGCAGCCCAACTGGCCGCAGCGAGGGCTGGGGCCACGTTTAATCGAACCGA‑‑TTAAACAACAACCTGCGGTGGATTTTCTTTCCGt  >  1:16077/1‑251 (MQ=255)                                                                                                                                                                                                     gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAaacaacaa                         <  1:437368/228‑1 (MQ=255)                                                                                                                                                                                                     gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACg                       >  1:323252/1‑230 (MQ=255)                                                                                                                                                                                                     gtgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCTGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGATTTTCTTTCCGt  >  1:598662/1‑251 (MQ=255)                                                                                                                                                                                                      tgtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGATCTTTGCGCATTTCCCGCATCGCCGTGCAGCCCAACTGGCAGCAGCGAGGGCCGGGGCAACGTTTAATCGAA‑C‑AGATTAAACAACAACGTGCGGTGGACTTTCTTTCCGt  >  1:85052/1‑250 (MQ=255)                                                                                                                                                                                                       gtgGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAATTCGTCAAATTCGACGCGGACAACGTCGCCTGCGCGGCAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCTTTGCGCATTTCCCGTATCGc                                                                                           >  1:830742/1‑162 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            GCGTTACGATGCCAACCTTGCCAAACAGATTCAGATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGAATCGGTTTATGGCTTGCTGACCTTGGCGCATTACCGAACCTCGCCGTTGGATTTACGTCGATTATTGGATGCGCCGCGACAACAATTTTATCTTGCGCAGACGCAGGAGGCCTTGTTGGGTTGTGTGTGGGCGGTGCCTGAGGGCAAATTTGGTGACGACGCATTAATTCGTAAAATTCGGCGCGGACAACGTCGTCCACGTGGTAATTTGGTGGCGCAAATGTTGTGTTTTCAGGCGGGATTGGAACAGGCGTGTAAATTGCGTTCACTGCGCATTTCCCGTATCGCCGTGCAACCCAACTGGCAGCAGCGGGGACTGGGGCAACGTTTAATCGAA‑CGAG‑TTAAACAACAACGTGCGGTGGATTTCCTTTCCGT  >  1/864583‑865029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑