Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 862,892 G→A intergenic (‑13235/‑59326) unknown ← / → unknown tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1862,8920GA100.0% 60.2 / NA 28intergenic (‑13235/‑59326)unknown/unknowntRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

CTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGCGT‑AAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACACGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAA  >  1/862671‑863137
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      
cttTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATAATTAATCTATTTCCACCCTGAAGAAATATCATGTAAAAAAAATCCTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                                                                                                                                                    <  1:189979/250‑1 (MQ=255)
      cGATCCGTATCTGAGCGAGAAGTAAAAAAAAGTCGAAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAACCGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTAAAAAAAATACTTATTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                                                                                                             <  1:386685/251‑1 (MQ=255)
       gATCCGTATCTGAGCGAGAAGTAAGAAAAAGGCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                                                                                                             <  1:43973/250‑1 (MQ=255)
        aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatc                                                                                                                                                                                                              >  1:444596/1‑248 (MQ=255)
        aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                                                                                                                                                    <  1:580545/242‑1 (MQ=255)
            gTATCTGAGCGAGAAGTAAGAAAAAGTCGGAGAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCg                                                                                                                                                                                                                                                >  1:42256/1‑210 (MQ=255)
             tATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCg                                                                                                                                                                                                      >  1:711569/1‑251 (MQ=255)
                                 aaaagtcggaaagtgaaaaaCTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATACGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                                                                                                                                                                                  <  1:223172/234‑1 (MQ=255)
                                             gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAAc                                                                                                                                                                                                                                  <  1:771184/186‑1 (MQ=255)
                                             gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcc                                                                                                                                                                            <  1:396556/239‑5 (MQ=255)
                                                         ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                         <  1:710923/251‑1 (MQ=255)
                                                             gaaaaacggggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                         <  1:143318/239‑1 (MQ=255)
                                                                               gggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                                                                                                             <  1:330856‑M2/148‑1 (MQ=255)
                                                                                                                     gccatatATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                         >  1:593899/3‑201 (MQ=255)
                                                                                                                        atatatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGatg                                                                                                                                                       <  1:762781/200‑3 (MQ=255)
                                                                                                                         tatatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCa                                                                                                   >  1:135765/1‑251 (MQ=255)
                                                                                                                          atatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                         >  1:819786/1‑196 (MQ=255)
                                                                                                                          atatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg                                                                                                  >  1:693307/1‑251 (MQ=255)
                                                                                                                          atatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg                                                                                                  >  1:231242/1‑251 (MQ=255)
                                                                                                                               gTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATc                                                                                              >  1:357354/1‑250 (MQ=255)
                                                                                                                                    acttaattcaggtttttAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTAAAGAAATATCATGTTAAAAAAATACTTACTTATTTTACCGTTTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                         <  1:840948/173‑1 (MQ=255)
                                                                                                                                    acGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGAt                                                                                        >  1:215132/1‑251 (MQ=255)
                                                                                                                                                                 gcgggacAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGaaaaa                                                           >  1:772262/7‑251 (MQ=255)
                                                                                                                                                                    ggacAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAAtt                                                         >  1:23965/4‑250 (MQ=255)
                                                                                                                                                                                    aTTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCAt                                                                                                                                                              >  1:409835/1‑133 (MQ=255)
                                                                                                                                                                                                  ggAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttgt                          >  1:417865/1‑251 (MQ=255)
                                                                                                                                                                                                                       acttacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTc                                                                                                                                   >  1:673166/1‑125 (MQ=255)
                                                                                                                                                                                                                        cttacttaTTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaag     >  1:586646/1‑250 (MQ=255)
                                                                                                                                                                                                                          tacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagcaa  >  1:664378/1‑251 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      
CTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGCGT‑AAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACACGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAA  >  1/862671‑863137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑