Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,813	A→G	intergenic (‑13156/‑59405)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,813	0	A	G	100.0%	127.8 / NA	48	intergenic (‑13156/‑59405)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

GGTGGTGCACGGCAGTTTGCATTTACTTGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCCTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGCGT‑AAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACA‑CGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGAT  >  1/862569‑863051                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 gggcacacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCtttt                                                                                                                                                                                                                                             >  1:530022‑M2/222‑251 (MQ=255)      cacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGt                                                                                                                                                                                                                                        >  1:115923‑M2/217‑251 (MQ=255)      cacatggtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGt                                                                                                                                                                                                                                        >  1:797600‑M2/217‑251 (MQ=255)            gtggtgcacggcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAg                                                                                                                                                                                                                                          >  1:897391‑M2/211‑243 (MQ=255)                      gcagtttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATACCGTAATTCAGGCTTTTAAGGTTGCTGcagcgggac                                                                                                                                                                                                                          <  1:618874‑M2/50‑5 (MQ=255)                           ttgcatttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGAtt                                                                                                                                                                                                                   <  1:845801‑M2/56‑1 (MQ=255)                               atttactcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATc                                                                                                                                                                                                               <  1:761325‑M2/60‑1 (MQ=255)                                   actcggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTAtt                                                                                                                                                                                                           >  1:66273‑M2/188‑251 (MQ=255)                                      cggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCttttaaggttgctgcacggaagggaccggat                                                                                                                                                                                                                  >  1:333148‑M2/185‑226 (MQ=255)                                       ggttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCa                                                                                                                                                                                                       >  1:472576‑M2/184‑251 (MQ=255)                                        gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTtgctgc                                                                                                                                                                                                                                  <  1:127641‑M2/41‑1 (MQ=255)                                        gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGAt                                                                                                                                                                                                                    >  1:150714‑M2/183‑237 (MQ=255)                                        gttatgatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATc                                                                                                                                                                                                               <  1:855640‑M2/60‑1 (MQ=255)                                             gatcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAAcctga                                                                                                                                                                                                 >  1:471959‑M2/178‑249 (MQ=255)                                              atcatattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAacctgaa                                                                                                                                                                                                >  1:446602‑M2/177‑248 (MQ=255)                                                 atattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGa                                                                                                                                                                                             >  1:690648‑M2/174‑251 (MQ=255)                                                 atattgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGa                                                                                                                                                                                             >  1:722156‑M2/174‑251 (MQ=255)                                                    ttgaagatgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAata                                                                                                                                                                                          <  1:426788‑M2/81‑1 (MQ=255)                                                          atgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGt                                                                                                                                                                                    <  1:842769‑M2/87‑1 (MQ=255)                                                           tgacgaagcggaggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAatat                                                                                                                                                                                         >  1:204574‑M2/164‑245 (MQ=255)                                                                      aggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAAtac                                                                                                                                                                         <  1:702070‑M2/98‑1 (MQ=255)                                                                       ggaaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAatat                                                                                                                                                                                         <  1:333155‑M2/82‑1 (MQ=255)                                                                         aaatggaaagcttggaaacgcaaattatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAAtacttac                                                                                                                                                                     <  1:187543‑M2/102‑1 (MQ=255)                                                                                                 ttatgatagggcttggctttgtcgatccgtatctgagcgagaagtaagaaaaagtcggaaagtgtaaaactatttcggaaaaacagggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAgg                                                                                                                                                                                                                                         >  1:366629‑M2/126‑159 (MQ=255)                                                                                                       cttTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATAATTAATCTATTTCCACCCTGAAGAAATATCATGTAAAAAAAATCCTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                                                              <  1:189979/250‑1 (MQ=255)                                                                                                             cGATCCGTATCTGAGCGAGAAGTAAAAAAAAGTCGAAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAACCGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTAAAAAAAATACTTATTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                       <  1:386685/251‑1 (MQ=255)                                                                                                              gATCCGTATCTGAGCGAGAAGTAAGAAAAAGGCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                       <  1:43973/250‑1 (MQ=255)                                                                                                               aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCt                                                                                                                                                                                                              <  1:460939/162‑1 (MQ=255)                                                                                                               aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatc                                                                                                                        >  1:444596/1‑248 (MQ=255)                                                                                                               aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCtgaagg                                                                                                                                                                                              <  1:391927/178‑6 (MQ=255)                                                                                                               aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                                                              <  1:580545/242‑1 (MQ=255)                                                                                                                   gTATCTGAGCGAGAAGTAAGAAAAAGTCGGAGAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCg                                                                                                                                                          >  1:42256/1‑210 (MQ=255)                                                                                                                    tATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCg                                                                                                                >  1:711569/1‑251 (MQ=255)                                                                                                                                        aaaagtcggaaagtgaaaaaCTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATA‑CGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                                                                                            <  1:223172/234‑1 (MQ=255)                                                                                                                                                gaaagtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAat                                                                                                                                                                                           <  1:424091/139‑1 (MQ=255)                                                                                                                                                    gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAAc                                                                                                                                            <  1:771184/186‑1 (MQ=255)                                                                                                                                                    gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcc                                                                                      <  1:396556/239‑5 (MQ=255)                                                                                                                                                                ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   <  1:710923/251‑1 (MQ=255)                                                                                                                                                                    gaaaaacggggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   <  1:143318/239‑1 (MQ=255)                                                                                                                                                                                      gggtcaaacgggcattcttgcttcagttgccattaaaagcCATATATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                                                                       <  1:330856‑M2/148‑1 (MQ=255)                                                                                                                                                                                                                            gccatatATGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   >  1:593899/3‑201 (MQ=255)                                                                                                                                                                                                                               atatatGGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGatg                                                                 <  1:762781/200‑3 (MQ=255)                                                                                                                                                                                                                                tatatgGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCa             >  1:135765/1‑251 (MQ=255)                                                                                                                                                                                                                                 atatgGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                   >  1:819786/1‑196 (MQ=255)                                                                                                                                                                                                                                 atatgGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg            >  1:693307/1‑251 (MQ=255)                                                                                                                                                                                                                                 atatgGTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg            >  1:231242/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTTATA‑CGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATc        >  1:357354/1‑250 (MQ=255)                                                                                                                                                                                                                                           a‑cGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGAt  >  1:215132/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GGTGGTGCACGGCAGTTTGCATTTACTTGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCCTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGCGT‑AAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACA‑CGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGAT  >  1/862569‑863051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑