Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,736	T→A	intergenic (‑13079/‑59482)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,736	0	T	A	98.6%	182.5 / NA	70	intergenic (‑13079/‑59482)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (36/33);  minor base C (0/1);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTTGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCCTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGCGT‑AAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACACGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGA  >  1/862502‑862986                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                             gtcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACggg                                                                                                                                                                                                                                                >  1:542021/3‑250 (MQ=255)  tcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCa                                                                                                                                                                                                                                              <  1:9290/250‑1 (MQ=255)         gtggtgGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTg                                                                                                                                                                                                                                        >  1:143813/1‑250 (MQ=255)                       ggctcaagagcaagagaaacCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATta                                                                                                                                                                                                                         >  1:148120‑M1/5‑249 (MQ=255)                        gctcaagagcaagagaaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaa                                                                                                                                                                                                                        >  1:606047‑M1/4‑248 (MQ=255)                              gagcaagagAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACg                                                                                                                                                                                                                                                  <  1:353159/219‑1 (MQ=255)                              gagcaagagAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGttg                                                                                                                                                                                                                               <  1:817617/238‑3 (MQ=255)                                 caagagAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatat                                                                                                                                                                                                               >  1:653899‑M1/1‑239 (MQ=255)                                  aagagAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatata                                                                                                                                                                                                              <  1:139343‑M1/251‑14 (MQ=255)                                       aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggt                                                                                                                                                                                                          <  1:591276‑M1/250‑18 (MQ=255)                                       aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatgg                                                                                                                                                                                                           <  1:411858‑M1/249‑17 (MQ=255)                                                          gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGttg                                                                                                                                                                                                                               <  1:645615/210‑3 (MQ=255)                                                          gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggctttt                                                                                                                                                                                      >  1:530022‑M1/1‑214 (MQ=255)                                                          gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggt                                                                                                                                                                                                          >  1:781562‑M1/1‑214 (MQ=255)                                                               cacaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggt                                                                                                                                                                                 >  1:115923‑M1/1‑209 (MQ=255)                                                               cacaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggt                                                                                                                                                                                 >  1:797600‑M1/1‑209 (MQ=255)                                                                acaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggct                                                                                                                                                                                         <  1:246222‑M1/242‑35 (MQ=255)                                                                 caTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggt                                                                                                                                                                                                          >  1:637499‑M1/1‑207 (MQ=255)                                                                   tggtggTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatat                                                                                                                                                                                                               >  1:192644‑M1/1‑205 (MQ=255)                                                                     gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAg                                                                                                                                                                                                                                                           >  1:169208/1‑171 (MQ=255)                                                                     gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCt                                                                                                                                                                                                                                      <  1:236684/192‑1 (MQ=255)                                                                     gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaag                                                                                                                                                                                   >  1:897391‑M1/1‑203 (MQ=255)                                                                     gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatat                                                                                                                                                                                                             >  1:348005‑M1/1‑203 (MQ=255)                                                                     gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatat                                                                                                                                                                                                               <  1:364300‑M1/215‑13 (MQ=255)                                                                               gCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttataccgtaattcaggcttttaaggttgctgcagcgggac                                                                                                                                                                  <  1:618874‑M1/250‑58 (MQ=255)                                                                                  gTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatat                                                                                                                                                                                                             >  1:497351‑M1/1‑190 (MQ=255)                                                                                    ttGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgatt                                                                                                                                                            <  1:845801‑M1/251‑64 (MQ=255)                                                                                      gCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatac                                                                                                                                                                                                     >  1:482943‑M1/1‑186 (MQ=255)                                                                                        aTTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatc                                                                                                                                                        <  1:761325‑M1/251‑68 (MQ=255)                                                                                            actcggttatGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctatt                                                                                                                                                    >  1:66273‑M1/5‑180 (MQ=255)                                                                                               cggtTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcacggaagggaccggat                                                                                                                                                           >  1:333148‑M1/2‑177 (MQ=255)                                                                                                ggTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttca                                                                                                                                                >  1:472576‑M1/1‑176 (MQ=255)                                                                                                 gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatc                                                                                                                                                        <  1:855640‑M1/242‑68 (MQ=255)                                                                                                 gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgat                                                                                                                                                             >  1:150714‑M1/1‑175 (MQ=255)                                                                                                 gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgc                                                                                                                                                                           <  1:127641‑M1/223‑49 (MQ=255)                                                                                                 gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcagg                                                                                                                                                                                           >  1:610940‑M1/1‑175 (MQ=255)                                                                                                 gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatgg                                                                                                                                                                                                           >  1:621619‑M1/1‑175 (MQ=255)                                                                                                      gATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctga                                                                                                                                          >  1:471959‑M1/1‑170 (MQ=255)                                                                                                       aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGt                                                                                                                                                                                                                                                        <  1:172796/140‑1 (MQ=255)                                                                                                       aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaa                                                                                                                                         >  1:446602‑M1/1‑169 (MQ=255)                                                                                                       aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgt                                                                                                                                                                                                   <  1:349224‑M1/193‑25 (MQ=255)                                                                                                          atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaacagccatatatggttatacgt                                                                                                                                                                                                   >  1:445310‑M1/1‑166 (MQ=255)                                                                                                          atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaagga                                                                                                                                      >  1:722156‑M1/1‑166 (MQ=255)                                                                                                          atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaagga                                                                                                                                      >  1:690648‑M1/1‑166 (MQ=255)                                                                                                            atTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgt                                                                                                                                                                                                   >  1:181142‑M1/1‑164 (MQ=255)                                                                                                            atTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatat                                                                                                                                                                                                             <  1:336420‑M1/178‑15 (MQ=255)                                                                                                             ttGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaata                                                                                                                                   <  1:426788‑M1/251‑89 (MQ=255)                                                                                                               gAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggt                                                                                                                                                                                                          >  1:780432‑M1/1‑161 (MQ=255)                                                                                                                   aTGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgt                                                                                                                             <  1:842769‑M1/251‑95 (MQ=255)                                                                                                                    tGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatat                                                                                                                                  >  1:204574‑M1/1‑156 (MQ=255)                                                                                                                               aggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatac                                                                                                                  <  1:702070‑M1/250‑106 (MQ=255)                                                                                                                                ggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatat                                                                                                                                  <  1:333155‑M1/233‑90 (MQ=255)                                                                                                                                  aaaTGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttac                                                                                                              <  1:187543‑M1/251‑110 (MQ=255)                                                                                                                                                        aaTTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgt                                                                                                                                                                                                   >  1:658073‑M1/1‑120 (MQ=255)                                                                                                                                                          ttATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggttatacgtaattcaggcttttaagg                                                                                                                                                                                  >  1:366629‑M1/1‑118 (MQ=255)                                                                                                                                                          ttATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATtaaaagccatatatggt                                                                                                                                                                                                          >  1:383742‑M1/1‑118 (MQ=255)                                                                                                                                                                          cttTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATAATTAATCTATTTCCACCCTGAAGAAATATCATGTAAAAAAAATCCTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                             <  1:189979/250‑1 (MQ=255)                                                                                                                                                                                cGATCCGTATCTGAGCGAGAAGTAAAAAAAAGTCGAAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAACCGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTAAAAAAAATACTTATTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                      <  1:386685/251‑1 (MQ=255)                                                                                                                                                                                 gATCCGTATCTGAGCGAGAAGTAAGAAAAAGGCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                      <  1:43973/250‑1 (MQ=255)                                                                                                                                                                                  aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCt                                                                                                                                             <  1:460939/162‑1 (MQ=255)                                                                                                                                                                                  aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatc                                                       >  1:444596/1‑248 (MQ=255)                                                                                                                                                                                  aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCtgaagg                                                                                                                             <  1:391927/178‑6 (MQ=255)                                                                                                                                                                                  aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                             <  1:580545/242‑1 (MQ=255)                                                                                                                                                                                      gTATCTGAGCGAGAAGTAAGAAAAAGTCGGAGAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCg                                                                                         >  1:42256/1‑210 (MQ=255)                                                                                                                                                                                       tATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCg                                               >  1:711569/1‑251 (MQ=255)                                                                                                                                                                                                           aaaagtcggaaagtgaaaaaCTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATACGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                           <  1:223172/234‑1 (MQ=255)                                                                                                                                                                                                                   gaaagtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAat                                                                                                                          <  1:424091/139‑1 (MQ=255)                                                                                                                                                                                                                       gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAAc                                                                           <  1:771184/186‑1 (MQ=255)                                                                                                                                                                                                                       gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcc                     <  1:396556/239‑5 (MQ=255)                                                                                                                                                                                                                                   ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa  <  1:710923/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                             GCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTTGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCCTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGCGT‑AAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACACGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGA  >  1/862502‑862986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑