Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,365	A→G	intergenic (‑12708/‑59853)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,365	0	A	G	100.0%	201.9 / NA	71	intergenic (‑12708/‑59853)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/27);  total (44/27)

GTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGCTGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTTGGTTATGATCAT  >  1/862128‑862608                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    gctgtGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGacg                                                                                                                                                                                                                                        >  1:32361/4‑249 (MQ=255)           taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTgcgc                                                                                                                                                                                                                                                  <  1:685900/228‑1 (MQ=255)           taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAg                                                                                                                                                                                                                              >  1:369172/4‑251 (MQ=255)            accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATtgtg                                                                                                                                                                                                                                            >  1:776726/3‑236 (MQ=255)                   ccgtgCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCAt                                                                                                                                                                                                                      >  1:48612/3‑251 (MQ=255)                                gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                                                                                                                                         >  1:458737/1‑251 (MQ=255)                                gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                                                                                                                                         >  1:4973/1‑251 (MQ=255)                                  gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTAt                                                                                                                                                                                                        >  1:823374/1‑250 (MQ=255)                                           atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                                                                                                                                                                                                                      >  1:408800/1‑211 (MQ=255)                                           atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                                                                                                                                                                                                                      >  1:257383/1‑211 (MQ=255)                                           atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCtcaa                                                                                                                                                                                                            <  1:856449/237‑4 (MQ=255)                                           atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAg                                                                                                                                                                                              >  1:526905/1‑251 (MQ=255)                                             cctatatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAGAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGAt                                                                                                                                                                                            >  1:430183/20‑251 (MQ=255)                                                atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                                                                                                                                         >  1:231436/17‑235 (MQ=255)                                                atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCg                                                                                                                                                                                          >  1:514291/17‑250 (MQ=255)                                                atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCg                                                                                                                                                                                          >  1:210651/17‑250 (MQ=255)                                                     ttgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCa                                                                                                                                                                                     >  1:583102/12‑250 (MQ=255)                                                        ctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTaa                                                                                                                                                                                 <  1:866114/243‑1 (MQ=255)                                                           gaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                               <  1:533243/245‑1 (MQ=255)                                                           gaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                               <  1:310007/245‑1 (MQ=255)                                                               gtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCg                                                                                                                                                                                                      >  1:549858/2‑223 (MQ=255)                                                               gtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGAt                                                                                                                                                                                            >  1:896642/2‑233 (MQ=255)                                                               gtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                               >  1:851079/2‑246 (MQ=255)                                                                    gCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCttt                                                                                                                                                                     >  1:897207/1‑251 (MQ=255)                                                                            cGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTg                                                                                                                                                             <  1:164981/251‑1 (MQ=255)                                                                                           aTGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAg                                                                                                                                              <  1:888932/251‑1 (MQ=255)                                                                                           aTGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAg                                                                                                                                              <  1:545251/251‑1 (MQ=255)                                                                                             gAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                                                                                                                                               >  1:195714/1‑216 (MQ=255)                                                                                             gAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGt                                                                                                                                             <  1:382044/250‑1 (MQ=255)                                                                                              aaTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATCCCCTGATGAAGTgg                                                                                                                                           >  1:770484/1‑251 (MQ=255)                                                                                               aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTg                                                                                                                                            <  1:127274/249‑1 (MQ=255)                                                                                               aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGa                                                                                                                                          >  1:643511/1‑251 (MQ=255)                                                                                               aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGa                                                                                                                                          >  1:462168/1‑251 (MQ=255)                                                                                                  tatgggAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGaa                                                                                                                                         >  1:764983/4‑248 (MQ=255)                                                                                                                gggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGc                                                                                                                          >  1:121083/1‑250 (MQ=255)                                                                                                                gggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCg                                                                                                                         >  1:282504/1‑251 (MQ=255)                                                                                                                 ggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCtgatga                                                                                                                                                >  1:80159/1‑227 (MQ=255)                                                                                                                 ggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGa                                                                                                                        >  1:2257/1‑251 (MQ=255)                                                                                                                    aaaatgatTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGAttt                                                                                                                     <  1:843914/248‑1 (MQ=255)                                                                                                                         gatTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTaa                                                                                                                                                                                 <  1:884109/186‑1 (MQ=255)                                                                                                                         gatTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGa                                                                                                                >  1:275563/1‑251 (MQ=255)                                                                                                                          attattGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGAt                                                                                                               >  1:190239/1‑251 (MQ=255)                                                                                                                                  tttACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTgtcgtc                                                                                                       <  1:546676/251‑1 (MQ=255)                                                                                                                                                     gATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAg                                                                                                    <  1:166424/235‑1 (MQ=255)                                                                                                                                                        gAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGc                                                                                                                                                        >  1:279376/1‑180 (MQ=255)                                                                                                                                                              ggCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTccc                                                                                                                                                                  <  1:673872/164‑1 (MQ=255)                                                                                                                                                                       acggcggagcAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAAcc                                                                  <  1:395898/243‑1 (MQ=255)                                                                                                                                                                                 aaaTTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTccc                                                                                                                                                                  <  1:405567/145‑1 (MQ=255)                                                                                                                                                                                        gcAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTggg                                                 >  1:174269/2‑251 (MQ=255)                                                                                                                                                                                           aTGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGca                                               >  1:797760/1‑250 (MQ=255)                                                                                                                                                                                              ggCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAg                                                                                            <  1:313794/202‑1 (MQ=255)                                                                                                                                                                                                cAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTACCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATCTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtg                                         >  1:216902/1‑251 (MQ=255)                                                                                                                                                                                                     gcggcAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                     <  1:141467/250‑1 (MQ=255)                                                                                                                                                                                                         cAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTcaagagcaagag                                                                       >  1:691261/1‑212 (MQ=255)                                                                                                                                                                                                            tGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCAc                                <  1:876441/248‑1 (MQ=255)                                                                                                                                                                                                              cAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGccc                                                                                                                                                      >  1:275061/1‑128 (MQ=255)                                                                                                                                                                                                                aCCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGtt                         >  1:67083/1‑251 (MQ=255)                                                                                                                                                                                                                        gtgatgAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTaa                                                                                                                                                                                 >  1:625758/6‑91 (MQ=255)                                                                                                                                                                                                                          gatgAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAt                                          >  1:674036/4‑224 (MQ=255)                                                                                                                                                                                                                              aagtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTggg                                                 <  1:761752/205‑1 (MQ=255)                                                                                                                                                                                                                                gtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGACCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTa         >  1:782267/7‑251 (MQ=255)                                                                                                                                                                                                                                gtggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAACGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACtc              >  1:576827/7‑245 (MQ=255)                                                                                                                                                                                                                                 tggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAtggtgg                                     <  1:147480/217‑1 (MQ=255)                                                                                                                                                                                                                                  ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTcaagagcaagag                                                                       <  1:384011/183‑1 (MQ=255)                                                                                                                                                                                                                                  ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACAt                                          <  1:863527/212‑1 (MQ=255)                                                                                                                                                                                                                                  ggaaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATg       >  1:769070/5‑251 (MQ=255)                                                                                                                                                                                                                                    aaaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGAt     >  1:141228/3‑251 (MQ=255)                                                                                                                                                                                                                                     aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc    <  1:318178/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc    <  1:662514/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaTGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATc    <  1:646851/250‑1 (MQ=255)                                                                                                                                                                                                                                       tGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCat  >  1:726057/1‑251 (MQ=255)                                                                                                                                                                                                                                         aCTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGtggtgg                                                                                              <  1:328432/157‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    GTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGCTGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTTGGTTATGATCAT  >  1/862128‑862608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑