Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,245	G→A	intergenic (‑12588/‑59973)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,245	0	G	A	100.0%	162.8 / NA	61	intergenic (‑12588/‑59973)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/20);  total (41/20)

GCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGCTGCTATTGGGCGA  >  1/862050‑862490                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                                                                                                  >  1:13317/1‑248 (MQ=255)          ttctttATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTACAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                                                                                                  >  1:688211/4‑239 (MQ=255)                     gggCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCaa                                                                                                                                                                           >  1:156731/3‑251 (MQ=255)                        cGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAcgg                                                                                                                                                                        <  1:876007/251‑1 (MQ=255)                             gATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCa                                                                                                                                                                   >  1:195459/1‑251 (MQ=255)                                  acacC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCa                                                                                                                                                              >  1:155591/1‑251 (MQ=255)                                    cacctttCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCaa                                                                                                                                                             >  1:532977/5‑251 (MQ=255)                                                attaaAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa                                                                                                                                                                                  <  1:468258/218‑1 (MQ=255)                                                    aaaaaCATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                                                                                             <  1:640682/251‑9 (MQ=255)                                                                 tctttCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAGTGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                                                                                             >  1:447021/3‑230 (MQ=255)                                                                               gctgtGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGacg                                                                                                                  >  1:32361/4‑249 (MQ=255)                                                                                         taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                                                                                             <  1:498878/211‑9 (MQ=255)                                                                                         taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTgcgc                                                                                                                            <  1:685900/228‑1 (MQ=255)                                                                                         taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAg                                                                                                        >  1:369172/4‑251 (MQ=255)                                                                                          accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAc                                                                                                                                                                          >  1:303120/3‑184 (MQ=255)                                                                                          accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAg                                                                                                                                                     >  1:144967/3‑205 (MQ=255)                                                                                          accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATtgtg                                                                                                                      >  1:776726/3‑236 (MQ=255)                                                                                           ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa                                                                                                                                                                                  <  1:626506/174‑1 (MQ=255)                                                                                           ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac                                                                                                                                   <  1:246810/221‑19 (MQ=255)                                                                                                 ccgtgCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCAt                                                                                                >  1:48612/3‑251 (MQ=255)                                                                                                     gCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac                                                                                                                                   >  1:69061/1‑194 (MQ=255)                                                                                                              gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                   >  1:4973/1‑251 (MQ=255)                                                                                                              gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                   >  1:458737/1‑251 (MQ=255)                                                                                                                gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                                                                                             <  1:138331/191‑9 (MQ=255)                                                                                                                gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTAt                                                                                  >  1:823374/1‑250 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                                                                                                  >  1:591108/1‑129 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                                                                                                >  1:408800/1‑211 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                                                                                                >  1:257383/1‑211 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCtcaa                                                                                      <  1:856449/237‑4 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAg                                                                        >  1:526905/1‑251 (MQ=255)                                                                                                                           cctatatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAGAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGAt                                                                      >  1:430183/20‑251 (MQ=255)                                                                                                                              atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAAc                                                                                                                                                            >  1:522133/17‑162 (MQ=255)                                                                                                                              atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAg                                                                                                                                                     <  1:218064/153‑1 (MQ=255)                                                                                                                              atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa                                                                                   >  1:231436/17‑235 (MQ=255)                                                                                                                              atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCg                                                                    >  1:514291/17‑250 (MQ=255)                                                                                                                              atatattgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCg                                                                    >  1:210651/17‑250 (MQ=255)                                                                                                                                   ttgctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCa                                                               >  1:583102/12‑250 (MQ=255)                                                                                                                                     gctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAg                                                                                                                                                     <  1:360669/153‑1 (MQ=255)                                                                                                                                      ctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAcgg                                                                                                                                                                        >  1:302464/9‑142 (MQ=255)                                                                                                                                      ctggaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTaa                                                           <  1:866114/243‑1 (MQ=255)                                                                                                                                         gaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                         <  1:310007/245‑1 (MQ=255)                                                                                                                                         gaacgtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                         <  1:533243/245‑1 (MQ=255)                                                                                                                                             gtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCg                                                                                >  1:549858/2‑223 (MQ=255)                                                                                                                                             gtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGAt                                                                      >  1:896642/2‑233 (MQ=255)                                                                                                                                             gtCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                         >  1:851079/2‑246 (MQ=255)                                                                                                                                                  gCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCttt                                               >  1:897207/1‑251 (MQ=255)                                                                                                                                                          cGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTg                                       <  1:164981/251‑1 (MQ=255)                                                                                                                                                                         aTGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAg                        <  1:545251/251‑1 (MQ=255)                                                                                                                                                                         aTGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAg                        <  1:888932/251‑1 (MQ=255)                                                                                                                                                                           gAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAAtg                                                         >  1:195714/1‑216 (MQ=255)                                                                                                                                                                           gAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGt                       <  1:382044/250‑1 (MQ=255)                                                                                                                                                                            aaTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATCCCCTGATGAAGTgg                     >  1:770484/1‑251 (MQ=255)                                                                                                                                                                             aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgt                                                                                                                                                    <  1:486571/123‑2 (MQ=255)                                                                                                                                                                             aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTg                      <  1:127274/249‑1 (MQ=255)                                                                                                                                                                             aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGa                    >  1:643511/1‑251 (MQ=255)                                                                                                                                                                             aTTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGa                    >  1:462168/1‑251 (MQ=255)                                                                                                                                                                                tatgggAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGaa                   >  1:764983/4‑248 (MQ=255)                                                                                                                                                                                              gggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGc    >  1:121083/1‑250 (MQ=255)                                                                                                                                                                                              gggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCg   >  1:282504/1‑251 (MQ=255)                                                                                                                                                                                               ggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCtgatga                          >  1:80159/1‑227 (MQ=255)                                                                                                                                                                                               ggTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGa  >  1:2257/1‑251 (MQ=255)                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGCTGCTATTGGGCGA  >  1/862050‑862490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑