Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,186	T→G	intergenic (‑12529/‑60032)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,186	0	T	G	100.0%	82.4 / NA	30	intergenic (‑12529/‑60032)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/9);  total (21/9)

GCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAG  >  1/862050‑862420                                                                                                                                         |                                                                                                                                                                                                                                           gCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                            >  1:13317/1‑248 (MQ=255)          ttctttATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTACAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                            >  1:688211/4‑239 (MQ=255)                     gggCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCaa                                                                                                     >  1:156731/3‑251 (MQ=255)                        cGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAcgg                                                                                                  <  1:876007/251‑1 (MQ=255)                             gATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCa                                                                                             >  1:195459/1‑251 (MQ=255)                                  acacC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCa                                                                                        >  1:155591/1‑251 (MQ=255)                                    cacctttCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCaa                                                                                       >  1:532977/5‑251 (MQ=255)                                                attaaAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa                                                                                                            <  1:468258/218‑1 (MQ=255)                                                    aaaaaCATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       <  1:640682/251‑9 (MQ=255)                                                                 tctttCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAGTGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       >  1:447021/3‑230 (MQ=255)                                                                               gctgtGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGacg                                            >  1:32361/4‑249 (MQ=255)                                                                                         taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       <  1:498878/211‑9 (MQ=255)                                                                                         taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTgcgc                                                      <  1:685900/228‑1 (MQ=255)                                                                                         taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAg                                  >  1:369172/4‑251 (MQ=255)                                                                                          accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAc                                                                                                    >  1:303120/3‑184 (MQ=255)                                                                                          accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAg                                                                               >  1:144967/3‑205 (MQ=255)                                                                                          accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATtgtg                                                >  1:776726/3‑236 (MQ=255)                                                                                           ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa                                                                                                            <  1:626506/174‑1 (MQ=255)                                                                                           ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac                                                             <  1:246810/221‑19 (MQ=255)                                                                                                 ccgtgCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCAt                          >  1:48612/3‑251 (MQ=255)                                                                                                     gCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac                                                             >  1:69061/1‑194 (MQ=255)                                                                                                              gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa             >  1:4973/1‑251 (MQ=255)                                                                                                              gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa             >  1:458737/1‑251 (MQ=255)                                                                                                                gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       <  1:138331/191‑9 (MQ=255)                                                                                                                gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTAt            >  1:823374/1‑250 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                            >  1:591108/1‑129 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                          >  1:408800/1‑211 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                          >  1:257383/1‑211 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCtcaa                <  1:856449/237‑4 (MQ=255)                                                                                                                         atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAg  >  1:526905/1‑251 (MQ=255)                                                                                                                                         |                                                                                                                                                                                                                                           GCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAG  >  1/862050‑862420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑