Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,175	G→A	intergenic (‑12518/‑60043)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,175	0	G	A	100.0%	83.5 / NA	32	intergenic (‑12518/‑60043)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/10);  total (22/10)

TGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAG  >  1/861927‑862420                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tgcatCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTatat                                                                                                                                                                                                                                                    <  1:384226/247‑1 (MQ=255)    atCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTGAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatat                                                                                                                                                                                                                                                  >  1:437414/2‑249 (MQ=255)    atCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCTGGGTGAAAATCATCCtatatatt                                                                                                                                                                                                                                                 >  1:299003/2‑249 (MQ=255)        aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatattg                                                                                                                                                                                                                                                >  1:83881/1‑245 (MQ=255)        aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatattg                                                                                                                                                                                                                                                >  1:489939/1‑245 (MQ=255)           ctctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATAttgctggaa                                                                                                                                                                                                                                          <  1:896675/251‑4 (MQ=255)             ctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATAttgctggaacg                                                                                                                                                                                                                                        >  1:396304/1‑246 (MQ=255)                                                                                                                            gCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                            >  1:13317/1‑248 (MQ=255)                                                                                                                                     ttctttATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTACAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                            >  1:688211/4‑239 (MQ=255)                                                                                                                                                gggCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCaa                                                                                                     >  1:156731/3‑251 (MQ=255)                                                                                                                                                   cGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAcgg                                                                                                  <  1:876007/251‑1 (MQ=255)                                                                                                                                                        gATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCa                                                                                             >  1:195459/1‑251 (MQ=255)                                                                                                                                                             acacC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCa                                                                                        >  1:155591/1‑251 (MQ=255)                                                                                                                                                               cacctttCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCaa                                                                                       >  1:532977/5‑251 (MQ=255)                                                                                                                                                                           attaaAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa                                                                                                            <  1:468258/218‑1 (MQ=255)                                                                                                                                                                               aaaaaCATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       <  1:640682/251‑9 (MQ=255)                                                                                                                                                                                            tctttCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAGTGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       >  1:447021/3‑230 (MQ=255)                                                                                                                                                                                                          gctgtGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGacg                                            >  1:32361/4‑249 (MQ=255)                                                                                                                                                                                                                    taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       <  1:498878/211‑9 (MQ=255)                                                                                                                                                                                                                    taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTgcgc                                                      <  1:685900/228‑1 (MQ=255)                                                                                                                                                                                                                    taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAg                                  >  1:369172/4‑251 (MQ=255)                                                                                                                                                                                                                     accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAc                                                                                                    >  1:303120/3‑184 (MQ=255)                                                                                                                                                                                                                     accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAg                                                                               >  1:144967/3‑205 (MQ=255)                                                                                                                                                                                                                     accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATtgtg                                                >  1:776726/3‑236 (MQ=255)                                                                                                                                                                                                                      ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa                                                                                                            <  1:626506/174‑1 (MQ=255)                                                                                                                                                                                                                      ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac                                                             <  1:246810/221‑19 (MQ=255)                                                                                                                                                                                                                            ccgtgCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCAt                          >  1:48612/3‑251 (MQ=255)                                                                                                                                                                                                                                gCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac                                                             >  1:69061/1‑194 (MQ=255)                                                                                                                                                                                                                                         gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa             >  1:4973/1‑251 (MQ=255)                                                                                                                                                                                                                                         gggTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTa             >  1:458737/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag                                                                       <  1:138331/191‑9 (MQ=255)                                                                                                                                                                                                                                           gTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTAt            >  1:823374/1‑250 (MQ=255)                                                                                                                                                                                                                                                    atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc                                                                                                                            >  1:591108/1‑129 (MQ=255)                                                                                                                                                                                                                                                    atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                          >  1:408800/1‑211 (MQ=255)                                                                                                                                                                                                                                                    atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACgag                                          >  1:257383/1‑211 (MQ=255)                                                                                                                                                                                                                                                    atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCtcaa                <  1:856449/237‑4 (MQ=255)                                                                                                                                                                                                                                                    atcCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCATTAAATCTCAATTATCGTGGCAAAg  >  1:526905/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCATTAAATCTTAATTATCGTGGCAAAG  >  1/861927‑862420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑