| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 862,158 | A→G | intergenic (‑12501/‑60060) | unknown ← / → unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 862,158 | 0 | A | G | 97.0% | 84.9 / NA | 33 | intergenic (‑12501/‑60060) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (21/11); minor base T (1/0); total (22/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCAT > 1/861915‑862396 | gagCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGaaa > 1:277556/1‑251 (MQ=255) cAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAatc < 1:245994/251‑1 (MQ=255) aTTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAatcat > 1:592584/1‑251 (MQ=255) aTTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAatcat > 1:706664/1‑251 (MQ=255) tttATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATcc < 1:277698/251‑1 (MQ=255) tgcatCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTatat < 1:384226/247‑1 (MQ=255) atCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTGAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatat > 1:437414/2‑249 (MQ=255) atCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCTGGGTGAAAATCATCCtatatatt > 1:299003/2‑249 (MQ=255) aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatattg > 1:489939/1‑245 (MQ=255) aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatattg > 1:83881/1‑245 (MQ=255) ctctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATAttgctggaa < 1:896675/251‑4 (MQ=255) ctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATAttgctggaacg > 1:396304/1‑246 (MQ=255) gCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc > 1:13317/1‑248 (MQ=255) ttctttATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTACAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGc > 1:688211/4‑239 (MQ=255) gggCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCaa > 1:156731/3‑251 (MQ=255) cGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAcgg < 1:876007/251‑1 (MQ=255) gATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCa > 1:195459/1‑251 (MQ=255) acacC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCa > 1:155591/1‑251 (MQ=255) cacctttCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCaa > 1:532977/5‑251 (MQ=255) attaaAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa < 1:468258/218‑1 (MQ=255) aaaaaCATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag < 1:640682/251‑9 (MQ=255) tctttCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAGTGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag > 1:447021/3‑230 (MQ=255) gctgtGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGacg > 1:32361/4‑249 (MQ=255) taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaag < 1:498878/211‑9 (MQ=255) taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTgcgc < 1:685900/228‑1 (MQ=255) taccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAg > 1:369172/4‑251 (MQ=255) accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAAc > 1:303120/3‑184 (MQ=255) accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAg > 1:144967/3‑205 (MQ=255) accTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATtgtg > 1:776726/3‑236 (MQ=255) ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCaa < 1:626506/174‑1 (MQ=255) ccTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac < 1:246810/221‑19 (MQ=255) ccgtgCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAGTGATGAAGTGGAAATGACTGTGCGCATTGTGGACGAGATGGAAAGCCACGCAt > 1:48612/3‑251 (MQ=255) gCAACTGCGGGGTGAAAATCATCCTATATATTGCTGGAACGTCGTGCAAAGTGCGGTTGGAAATGGCAATGAATTTATGGGAGCAACATGGGTAAAATGATTATTGATTTACAAATCGCCAGCGCAGATGAAAATGGCTTGCCGACGGCGGAGCAAATTGAGCAATGGGCAACGGCGGCAGTGCAACCGCAAAgtgatgaagtggaaatgac > 1:69061/1‑194 (MQ=255) | GAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAACTCGTGCAAAATGCGGTCGGAAATGGCGATGAATTTAGGGGAGCAACATGGGTAAGATGATTATTGATTTACAAATCGCCTGCGCAGATGAAAATGGCTTGCCGACGGCAGCGCAAATTGAACAATGGGCAACGGCGGCAGTGCAACCGCAAAGCGGGGAAGTTGAGATGACTGTACGCATTGTGGATGAGACGGAAAGCCACGCAT > 1/861915‑862396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |