Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,995	G→A	intergenic (‑12338/‑60223)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,995	0	G	A	100.0%	84.3 / NA	33	intergenic (‑12338/‑60223)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

TACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAAC  >  1/861782‑862190                                                                                                                                                                                                                        |                                                                                                                                                                                                    caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACtt                                                                                                                                                                        <  1:578954/243‑1 (MQ=255)   cTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTg                                                                                                                                                               >  1:563103/1‑251 (MQ=255)           tgtgtgGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaacaagca                                                                                                                                                                                      <  1:813579/220‑4 (MQ=255)                   tttAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaa                                                                                                                                                                                            <  1:702739/206‑1 (MQ=255)                                                                            cTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACa                                                                                     >  1:48135/1‑251 (MQ=255)                                                                             ttttttAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACAt                                                                                    <  1:552195/251‑1 (MQ=255)                                                                              tttttAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGaacc                                                                                                                                                           <  1:207156/180‑3 (MQ=255)                                                                                                                    aaaaaGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaac                                                                                                                                                                                           <  1:725840/110‑1 (MQ=255)                                                                                                                         gTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTAc                                              >  1:488328/1‑245 (MQ=255)                                                                                                                           tatatttATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCa                                        <  1:407675/246‑1 (MQ=255)                                                                                                                              atttATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCcgctgtgggt                                                        >  1:408661/2‑223 (MQ=255)                                                                                                                              atttATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTAccg                                            >  1:194500/2‑241 (MQ=255)                                                                                                                              atttATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTAccg                                            >  1:787465/2‑241 (MQ=255)                                                                                                                                        gagCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGaaa                          >  1:277556/1‑251 (MQ=255)                                                                                                                                           cAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAatc                       <  1:245994/251‑1 (MQ=255)                                                                                                                                             aTTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACAttttgatctttctg                                                                       <  1:720724/201‑11 (MQ=255)                                                                                                                                             aTTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAatcat                     >  1:706664/1‑251 (MQ=255)                                                                                                                                             aTTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAatcat                     >  1:592584/1‑251 (MQ=255)                                                                                                                                             aTTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGACGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCcgctgtgggtttacc                                                   >  1:644614/1‑208 (MQ=255)                                                                                                                                               tttATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATcc                   <  1:277698/251‑1 (MQ=255)                                                                                                                                                    tgcatCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTatat              <  1:384226/247‑1 (MQ=255)                                                                                                                                                      catCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCActc                                                                                                                                                                  <  1:643493/100‑1 (MQ=255)                                                                                                                                                      catCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAaca                                                                                                           <  1:333472/155‑1 (MQ=255)                                                                                                                                                       atCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTGAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatat            >  1:437414/2‑249 (MQ=255)                                                                                                                                                       atCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCTGGGTGAAAATCATCCtatatatt           >  1:299003/2‑249 (MQ=255)                                                                                                                                                           agtgtctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGa                                                                                                                 <  1:129475/142‑1 (MQ=255)                                                                                                                                                           aatctcTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGGGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTAc                                              <  1:320234/210‑1 (MQ=255)                                                                                                                                                           aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAGCCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAac                                                                                                            >  1:463876/1‑151 (MQ=255)                                                                                                                                                           aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTAc                                                                                                                                       <  1:408369/124‑1 (MQ=255)                                                                                                                                                           aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatattg          >  1:489939/1‑245 (MQ=255)                                                                                                                                                           aGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCtatatattg          >  1:83881/1‑245 (MQ=255)                                                                                                                                                              ctctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATAttgctggaa    <  1:896675/251‑4 (MQ=255)                                                                                                                                                                ctTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACATTTTGATCTTTCTGTTGCCCGCTGTGGGTTTACCTTTACCGTGCAACTGCGGGGTGAAAATCATCCTATATAttgctggaacg  >  1:396304/1‑246 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                    TACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACATTTTAGTTTTTCTGTTACCCGTCGTGGTTTTATCTTTACTGTGCAATTGCAGGGTGAAAATCATCCTGTATACTGCTGTAAAC  >  1/861782‑862190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑