Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,809	G→A	intergenic (‑12152/‑60409)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,809	0	G	A	100.0%	36.5 / NA	15	intergenic (‑12152/‑60409)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

TAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTG  >  1/861565‑862033                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   taataaGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATcgcg                                                                                                                                                                                                                               >  1:354355/1‑251 (MQ=255)                            accggtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGaa                                                                                                                                                                                                     <  1:232544/244‑1 (MQ=255)                                gtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTC‑ttttttct                                                                                                                                                                                                             >  1:56866/3‑234 (MQ=255)                                                                                        gTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCT‑aaaaaa                                                                                                                                       >  1:895687/1‑250 (MQ=255)                                                                                                 cgtatTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttta                                                                                                                               >  1:512730/2‑250 (MQ=255)                                                                                                                          aattaaCAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTttaact                                                                                                                                                                          <  1:234983/183‑1 (MQ=255)                                                                                                                                                        tGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg                                                                       <  1:654655/251‑1 (MQ=255)                                                                                                                                                              aTTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGc                                                                  >  1:276276/1‑250 (MQ=255)                                                                                                                                                                                                                      caaaaaCACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTg                                                                                                           <  1:438452/153‑1 (MQ=255)                                                                                                                                                                                                                            caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttt                                                                                                                                >  1:104600/2‑128 (MQ=255)                                                                                                                                                                                                                            caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg                                                                       >  1:66297/2‑184 (MQ=255)                                                                                                                                                                                                                            caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACtt           <  1:578954/243‑1 (MQ=255)                                                                                                                                                                                                                              cTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTg  >  1:563103/1‑251 (MQ=255)                                                                                                                                                                                                                                      tgtgtgGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaacaagca                         <  1:813579/220‑4 (MQ=255)                                                                                                                                                                                                                                              tttAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaa                               <  1:702739/206‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   TAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTG  >  1/861565‑862033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑