Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,782	T→C	intergenic (‑12125/‑60436)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,782	0	T	C	100.0%	22.8 / NA	10	intergenic (‑12125/‑60436)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/5);  total (5/5)

TAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGC  >  1/861565‑861969                                                                                                                                                                                                                            |                                                                                                                                                                                              taataaGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATcgcg                                                                                                                                                               >  1:354355/1‑251 (MQ=255)                            accggtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGaa                                                                                                                                     <  1:232544/244‑1 (MQ=255)                                gtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTC‑ttttttct                                                                                                                                             >  1:56866/3‑234 (MQ=255)                                                                                        gTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCT‑aaaaaa                                                                       >  1:895687/1‑250 (MQ=255)                                                                                                 cgtatTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttta                                                               >  1:512730/2‑250 (MQ=255)                                                                                                                          aattaaCAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTttaact                                                                                                          <  1:234983/183‑1 (MQ=255)                                                                                                                                                aTTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAata                                                                                                                                                                                      <  1:378869/85‑1 (MQ=255)                                                                                                                                                        tGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg       <  1:654655/251‑1 (MQ=255)                                                                                                                                                              aTTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGc  >  1:276276/1‑250 (MQ=255)                                                                                                                                                                                                                      caaaaaCACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTg                                           <  1:438452/153‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                              TAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGC  >  1/861565‑861969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑