Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,732	C→T	intergenic (‑12075/‑60486)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,732	0	C	T	100.0%	32.6 / NA	14	intergenic (‑12075/‑60486)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/6);  total (8/6)

GTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAATGTCATTTATTTAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGC  >  1/861491‑861969                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGa                                                                                                                                                                                                                                          >  1:225005/1‑251 (MQ=255)                                                                           taataaGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATcgcg                                                                                                                                                               >  1:354355/1‑251 (MQ=255)                                                                                                      accggtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGaa                                                                                                                                     <  1:232544/244‑1 (MQ=255)                                                                                                          gtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTC‑ttttttct                                                                                                                                             >  1:56866/3‑234 (MQ=255)                                                                                                                                                      taaATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTAc                                                                                                                                                                                                                         <  1:146277/119‑1 (MQ=255)                                                                                                                                                                atgTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACt                                                                                                                                                                                                              <  1:180167/118‑1 (MQ=255)                                                                                                                                                                atgTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACaaaaacacttaaa                                                                                                                                                                                         >  1:385564/3‑133 (MQ=255)                                                                                                                                                                  gTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCT‑aaaaaa                                                                       >  1:895687/1‑250 (MQ=255)                                                                                                                                                                           cgtatTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttta                                                               >  1:512730/2‑250 (MQ=255)                                                                                                                                                                                                    aattaaCAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTttaact                                                                                                          <  1:234983/183‑1 (MQ=255)                                                                                                                                                                                                              aTGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTAc                                                                                                                                                                                                                         >  1:322714/1‑63 (MQ=255)                                                                                                                                                                                                                          aTTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAata                                                                                                                                                                                      <  1:378869/85‑1 (MQ=255)                                                                                                                                                                                                                                  tGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg       <  1:654655/251‑1 (MQ=255)                                                                                                                                                                                                                                        aTTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGc  >  1:276276/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                GTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAATGTCATTTATTTAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGC  >  1/861491‑861969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑