Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,553	A→G	intergenic (‑11896/‑60665)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,553	0	A	G	100.0%	78.5 / NA	29	intergenic (‑11896/‑60665)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/18);  total (11/18)

TTTGTAAGCCCTTAATAAAACCATTGTCGCTGTAGAATACAGCAACGATTAACACGGCAACAGCAATGGCGATCAAACCGTATTCAATTGCAGTCACACCTGCTTGATTTTTTTTGAAAGAACGGATTGCTTCGGTTGCTTTGATGTATGCTTTCGTAATTAAGGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAATGTCATTTATTTAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGA  >  1/861315‑861739                                                                                                                                                                                                                                               |                                                                                                                                                                                             tttgtaagttagcgataaAACCATTGTTGCTGTAGAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCatttattt                                                                                                                                                                                  <  1:528346/237‑1 (MQ=255)         ttagcgataaAACCATTGTTGCTGTAGAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTtaataagct                                                                                                                                                                          <  1:164569/245‑4 (MQ=255)               ataaAACCATTGTTGCTGTAGAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGa                                                                                                                                                                    >  1:714491/1‑251 (MQ=255)                  aaaCCATTGTTGCTGTAGAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTgg                                                                                                                                                                     >  1:488632/1‑247 (MQ=255)                           ttgCTGTAGAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAgtgt                                                                                                                                                                                           <  1:773810/214‑1 (MQ=255)                                gTAGAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccgg                                                                                                                                                   >  1:558000/1‑247 (MQ=255)                                   gAATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccggtat                                                                                                                                                >  1:28335/1‑244 (MQ=255)                                     aTACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccggtatat                                                                                                                                              <  1:884156/251‑10 (MQ=255)                                     aTACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccggtatat                                                                                                                                              <  1:706114/251‑10 (MQ=255)                                     aTACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccggtatat                                                                                                                                              <  1:627293/251‑10 (MQ=255)                                        ctgCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGt                                                                                                                                           <  1:468214/249‑1 (MQ=255)                                               gATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGaaa                                                                                                                                    >  1:625438/1‑251 (MQ=255)                                                             cAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGt                                                                                                                                           <  1:664083/230‑1 (MQ=255)                                                             cAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGt                                                                                                                                           <  1:253314/230‑1 (MQ=255)                                                               gCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTtaataag                                                                                                                                                                            <  1:16545/195‑2 (MQ=255)                                                                        aTCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTtaataa                                                                                                                                                                             <  1:54615/185‑1 (MQ=255)                                                                        aTCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccggtatat                                                                                                                                              >  1:318791/1‑207 (MQ=255)                                                                                           cAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGt                                                                                                                                           <  1:17186/200‑1 (MQ=255)                                                                                              tCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTAtatg                                                                                     <  1:110562/251‑1 (MQ=255)                                                                                                                                 gcttcagctGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGaaa                                                                                                                                    >  1:424453/9‑169 (MQ=255)                                                                                                                                          gCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGaccggtatatag                                                                                                                                            <  1:411900/152‑12 (MQ=255)                                                                                                                                                     tGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTa                              <  1:484344/251‑1 (MQ=255)                                                                                                                                                                     gTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTc                                                                                                                                          <  1:757877/127‑1 (MQ=255)                                                                                                                                                                     gTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatgta                                                                                   <  1:382401/182‑1 (MQ=255)                                                                                                                                                                       aTTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGt                                                                                                                                           >  1:497720/1‑124 (MQ=255)                                                                                                                                                                               ttGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGaat                                                                                                          >  1:568183/1‑149 (MQ=255)                                                                                                                                                                                 gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTAtatg                                                                                     <  1:566836/168‑1 (MQ=255)                                                                                                                                                                                 gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGtt                                           <  1:199993/210‑1 (MQ=255)                                                                                                                                                                                 gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGa  >  1:225005/1‑251 (MQ=255)                                                                                                                                                                                   cTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAggg                                      >  1:497732/1‑213 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                             TTTGTAAGCCCTTAATAAAACCATTGTCGCTGTAGAATACAGCAACGATTAACACGGCAACAGCAATGGCGATCAAACCGTATTCAATTGCAGTCACACCTGCTTGATTTTTTTTGAAAGAACGGATTGCTTCGGTTGCTTTGATGTATGCTTTCGTAATTAAGGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAATGTCATTTATTTAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGA  >  1/861315‑861739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑