Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,095	G→A	intergenic (‑11438/‑61123)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,095	0	G	A	100.0%	89.8 / NA	34	intergenic (‑11438/‑61123)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

GTTACTAATAATCCGAGAACGAATGTCAGTTACAGATAAAGTAGTCAGCAGTAAGATCGTTATGACTACTAAAGTATTAATAAGCCAGTTCATTTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGAGACAGTGGTCGTTAAATCCGAGAACTTACCTGACAACGCCTTAATAAAGCTGTTATCCTCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAGCCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACTTACACGAAAAAAAGACTACTTGACGATAAAAGTAATCCAGTAAATCCATCATAATTAATTCTAATATAGAGTGTAGAATAGCTC  >  1/860852‑861223                                                                                                                                                                                                                                                    |                                                                                                                                 gTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATa                                                                                                                            >  1:724868/1‑249 (MQ=255) gTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATAc                                                                                                                           <  1:359386/250‑1 (MQ=255)        ataagCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGGTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGa                                                                                                                   <  1:430098/251‑1 (MQ=255)          aagccGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAg                                                                                                                 <  1:674379/248‑1 (MQ=255)                          tCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGAt                                                                                                  <  1:311721/250‑1 (MQ=255)                             gTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      >  1:462671/1‑227 (MQ=255)                                 taGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATAc                                                                                                                           <  1:857845/217‑1 (MQ=255)                                 taGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTaa                                                                                          >  1:291182/2‑251 (MQ=255)                                  aGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTaaa                                                                                         >  1:858182/1‑251 (MQ=255)                                    aTAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAAc                                                                                       <  1:366220/251‑1 (MQ=255)                                      aaagtaaccagcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAAccc                                                                                                                               >  1:663397/21‑209 (MQ=255)                                          taaccagcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAAcctatac                                                                                 <  1:482912/235‑7 (MQ=255)                                                gcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGaagaa                                                                           >  1:2621/11‑248 (MQ=255)                                                                gattactaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTgatgat                                                           >  1:835872/4‑251 (MQ=255)                                                                  ttactaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTc                                                                                                     >  1:281763/2‑207 (MQ=255)                                                                  ttactaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGact                                                                     <  1:149626/238‑1 (MQ=255)                                                                    actaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGatga                                                            <  1:190674/246‑4 (MQ=255)                                                                     ctaaagcgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      <  1:583085/179‑1 (MQ=255)                                                                           cgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATTCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATcc                                                <  1:452470/249‑1 (MQ=255)                                                                           cgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATcc                                                <  1:230394/249‑1 (MQ=255)                                                                              taataaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      <  1:869767/178‑1 (MQ=255)                                                                              taataaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTg                                                                <  1:263358/232‑1 (MQ=255)                                                                              taataaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATAATCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTg                                                                >  1:757379/1‑232 (MQ=255)                                                                                 taaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTaaa                                          <  1:599507/251‑1 (MQ=255)                                                                                     ccAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATc                                        <  1:886480/249‑1 (MQ=255)                                                                                                     tCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttca                       >  1:159307/1‑248 (MQ=255)                                                                                                                       tttGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttcaaaatgaagcaaagaatag     >  1:392439/1‑230 (MQ=255)                                                                                                                          gCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttcaaaatgaagcaaagaatagcc   >  1:62568/1‑227 (MQ=255)                                                                                                                           cTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttcaaaatgaagcaaagaatagccc  <  1:880613/250‑25 (MQ=255)                                                                                                                                                                       aCCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACt                                                                                                                          <  1:864982/86‑1 (MQ=255)                                                                                                                                                                                     taataaAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAg                                                      >  1:214149/1‑140 (MQ=255)                                                                                                                                                                                            gCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      >  1:519820/1‑69 (MQ=255)                                                                                                                                                                                                                      cTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      <  1:209814/43‑1 (MQ=255)                                                                                                                                                                                                                                              cAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAg                                                      >  1:371830/1‑83 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                 GTTACTAATAATCCGAGAACGAATGTCAGTTACAGATAAAGTAGTCAGCAGTAAGATCGTTATGACTACTAAAGTATTAATAAGCCAGTTCATTTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGAGACAGTGGTCGTTAAATCCGAGAACTTACCTGACAACGCCTTAATAAAGCTGTTATCCTCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAGCCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACTTACACGAAAAAAAGACTACTTGACGATAAAAGTAATCCAGTAAATCCATCATAATTAATTCTAATATAGAGTGTAGAATAGCTC  >  1/860852‑861223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑