Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,049	T→C	intergenic (‑11392/‑61169)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,049	0	T	C	100.0%	115.3 / NA	43	intergenic (‑11392/‑61169)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/26);  total (17/26)

AATAAACTGAACGGAATAATGACGAATAACAGAAACAAAACGACTCGGTTACTAATAATCCGAGAACGAATGTCAGTTACAGATAAAGTAGTCAGCAGTAAGATCGTTATGACTACTAAAGTATTAATAAGCCAGTTCATTTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGAGACAGTGGTCGTTAAATCCGAGAACTTACCTGACAACGCCTTAATAAAGCTGTTATCCTCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAGCCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACTTACACGAAAAAAAGACTACTTGACGATAAAAGTAATCCAGTAAATCCATCATAATTAATTCTAATATAGAGTGTAGAATAGCTC  >  1/860805‑861223                                                                                                                                                                                                                                                     |                                                                                                                                                                               aataaACTGAACGGAATAATGACGAATAACAGAAACAAAACAATTCGGTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTaa                                                                                                                                                                         >  1:843970/1‑251 (MQ=255)    aaaCTGAACGGAATAATGACGAATAACAGAAACAAAACAATTCGGTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAAc                                                                                                                                                                      <  1:725234/251‑1 (MQ=255)                   atGACGAATAACAGAAACAAAACAATTCGGTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAAc                                                                                                                                                       <  1:85047/251‑1 (MQ=255)                   atGACGAATAACAGAAACAAAACAATTCGGTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAAc                                                                                                                                                       <  1:261506/251‑1 (MQ=255)                                          aattCGGTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTaacc                                                                                                                                <  1:779923/248‑4 (MQ=255)                                                gTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATa                                                                                                                            >  1:724868/1‑249 (MQ=255)                                                gTTACTAATAAGCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATAc                                                                                                                           <  1:359386/250‑1 (MQ=255)                                                       ataagCCGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGGTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGa                                                                                                                   <  1:430098/251‑1 (MQ=255)                                                         aagccGAAAACGAATGTCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAg                                                                                                                 <  1:674379/248‑1 (MQ=255)                                                                         tCAGTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGAt                                                                                                  <  1:311721/250‑1 (MQ=255)                                                                            gTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTaacc                                                                                                                                >  1:500053/1‑214 (MQ=255)                                                                            gTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTaac                                                                                                                                 <  1:152763/216‑3 (MQ=255)                                                                            gTTATAGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      >  1:462671/1‑227 (MQ=255)                                                                                taGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATAc                                                                                                                           <  1:857845/217‑1 (MQ=255)                                                                                taGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTaa                                                                                          >  1:291182/2‑251 (MQ=255)                                                                                 aGATAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTaaa                                                                                         >  1:858182/1‑251 (MQ=255)                                                                                   aTAAAGTAACCAGCAGCGAGAGCGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAAc                                                                                       <  1:366220/251‑1 (MQ=255)                                                                                     aaagtaaccagcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAAccc                                                                                                                               >  1:663397/21‑209 (MQ=255)                                                                                         taaccagcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAAcctatac                                                                                 <  1:482912/235‑7 (MQ=255)                                                                                            ccagcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGc                                                                                                                                        <  1:215862/180‑1 (MQ=255)                                                                                               gcagcgagagcGTTATGATTACTAAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGaagaa                                                                           >  1:2621/11‑248 (MQ=255)                                                                                                               gattactaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTgatgat                                                           >  1:835872/4‑251 (MQ=255)                                                                                                                 ttactaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTc                                                                                                     >  1:281763/2‑207 (MQ=255)                                                                                                                 ttactaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGact                                                                     <  1:149626/238‑1 (MQ=255)                                                                                                                   actaAAGCGTTAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGatga                                                            <  1:190674/246‑4 (MQ=255)                                                                                                                    ctaaagcgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      <  1:583085/179‑1 (MQ=255)                                                                                                                         gcgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTaacc                                                                                                                                >  1:463532/4‑169 (MQ=255)                                                                                                                          cgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATTCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATcc                                                <  1:452470/249‑1 (MQ=255)                                                                                                                          cgttAATAAGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATcc                                                <  1:230394/249‑1 (MQ=255)                                                                                                                             taataaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      <  1:869767/178‑1 (MQ=255)                                                                                                                             taataaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTg                                                                <  1:263358/232‑1 (MQ=255)                                                                                                                             taataaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATAATCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTg                                                                >  1:757379/1‑232 (MQ=255)                                                                                                                                taaGCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTaaa                                          <  1:599507/251‑1 (MQ=255)                                                                                                                                   gCCAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTa                                                                                                                                   <  1:825586/159‑1 (MQ=255)                                                                                                                                    ccAGTTCA‑TTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATc                                        <  1:886480/249‑1 (MQ=255)                                                                                                                                                    tCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttca                       >  1:159307/1‑248 (MQ=255)                                                                                                                                                                      tttGCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttcaaaatgaagcaaagaatag     >  1:392439/1‑230 (MQ=255)                                                                                                                                                                         gCTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttcaaaatgaagcaaagaatagcc   >  1:62568/1‑227 (MQ=255)                                                                                                                                                                          cTAACTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAGTAATCCAGTAAATCCATCATaattaatttcaaaatgaagcaaagaatagccc  <  1:880613/250‑25 (MQ=255)                                                                                                                                                                             aCTATGGCGTCAGTGACAGTGGTCGTTAAATCCGAGAACTTACCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAAc                                                                                                                                                       <  1:698891/98‑1 (MQ=255)                                                                                                                                                                                                                      aCCTGACACTGCCTTAATAAAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACt                                                                                                                          <  1:864982/86‑1 (MQ=255)                                                                                                                                                                                                                                    taataaAGCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACCTATACGAAGAAAAGACTACTTGATGATAAAAg                                                      >  1:214149/1‑140 (MQ=255)                                                                                                                                                                                                                                           gCTGTTATCCCCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAACCCATACTCAAc                                                                                                                      >  1:519820/1‑69 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                               AATAAACTGAACGGAATAATGACGAATAACAGAAACAAAACGACTCGGTTACTAATAATCCGAGAACGAATGTCAGTTACAGATAAAGTAGTCAGCAGTAAGATCGTTATGACTACTAAAGTATTAATAAGCCAGTTCATTTTTTTATCATTTTTCTTAGCTACTTTTGCTAACTATGGCGTCAGAGACAGTGGTCGTTAAATCCGAGAACTTACCTGACAACGCCTTAATAAAGCTGTTATCCTCGACTAAAACGGCGACTACAAAAACAGCAATAGCCACAGCAATTAGCCCATACTCAACAGAAGTAATCCCTTGTCGATTTCTGTAAAACTTACACGAAAAAAAGACTACTTGACGATAAAAGTAATCCAGTAAATCCATCATAATTAATTCTAATATAGAGTGTAGAATAGCTC  >  1/860805‑861223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑