Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	860,279	T→C	intergenic (‑10622/‑61939)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	860,279	0	T	C	100.0%	166.1 / NA	61	intergenic (‑10622/‑61939)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/36);  total (25/36)

TGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACTAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATATAATCATCTGCCTGTAATAAATGACCTTTGCTTACGTCATTCTTTAACTCGGCAGTTAAAATTAGCTTTTCCGGTTTTTTCTCAGCTTCCGGAACCTGCTGATTATTTATTATTGTTTGTTGATTTTCAGTATTGTCAAATGTTGGCATAAACATAATGCCACCAATGCCAACCGCTAGCGTAAGCACTGAAATGATCAAGAGCGTTCTGTAATTCATTTTTTACCCTTAATTAAATTTTAACAAAGTAAAGTCTCCGCTCAATCAAAATTAATTTGA  >  1/860046‑860524                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      tgacttggattcaagCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTTTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTaattc                                                                                                                                                                                                                                      <  1:426618/237‑4 (MQ=255)    cttggattcaagCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCCCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGc                                                                                                                                                                                                                                   <  1:814100/240‑1 (MQ=255)                   gaagTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTaat                                                                                                                                                                                                                                        >  1:324211/4‑230 (MQ=255)                             aTCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATATCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTc                                                                                                                                                                                                                                                  <  1:486708/211‑1 (MQ=255)                             aTCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCttt                                                                                                                                                                                                                                           >  1:708772/1‑218 (MQ=255)                             aTCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGcttt                                                                                                                                                                                                          >  1:814244/1‑251 (MQ=255)                                               agaTAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTaat                                                                                                                                                                                                                                        <  1:563279/203‑2 (MQ=255)                                                gaTAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAAccg                                                                                                                                                                                       >  1:665572/1‑250 (MQ=255)                                                gaTAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAAccg                                                                                                                                                                                       >  1:279032/1‑250 (MQ=255)                                                gaTAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAAc                                                                                                                                                                                         >  1:105153/1‑249 (MQ=255)                                                        aTTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGatta                                                                                                                                                                               <  1:485190/251‑1 (MQ=255)                                                        aTTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGatt                                                                                                                                                                                <  1:11954/250‑1 (MQ=255)                                                             ttgGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATtta                                                                                                                                                                           <  1:90435‑M1/250‑4 (MQ=255)                                                             ttgGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATtta                                                                                                                                                                           <  1:100097‑M1/250‑4 (MQ=255)                                                                     aaaaGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattg                                                                                                                                                                  <  1:732558‑M1/251‑13 (MQ=255)                                                                     aaaaGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattg                                                                                                                                                                  <  1:133137‑M1/251‑13 (MQ=255)                                                                         gAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttg                                                                                                                                                              >  1:828673‑M1/1‑235 (MQ=255)                                                                               gCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGa                                                                                                                                                                                           >  1:347805/1‑216 (MQ=255)                                                                               gCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgatt                                                                                                                                                        >  1:376894‑M1/1‑229 (MQ=255)                                                                               gCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATtta                                                                                                                                                                           <  1:242615‑M1/232‑4 (MQ=255)                                                                                       aTTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtat                                                                                                                                                <  1:873148‑M1/251‑31 (MQ=255)                                                                                                  ttAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATtta                                                                                                                                                                           <  1:106041‑M1/213‑4 (MQ=255)                                                                                                         gCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCttt                                                                                                                                                                                                                                           <  1:407299/142‑1 (MQ=255)                                                                                                         gCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattg                                                                                                                                                                  <  1:641245‑M1/215‑13 (MQ=255)                                                                                                           cTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaac                                                                                                                            <  1:150762‑M1/251‑51 (MQ=255)                                                                                                              gTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattg                                                                                                                                                                  <  1:504269‑M1/210‑13 (MQ=255)                                                                                                                 aTCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatg                                                                                                                      <  1:481173‑M1/251‑57 (MQ=255)                                                                                                                    ccTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcag                                                                                                                                                   <  1:37633‑M1/219‑28 (MQ=255)                                                                                                                                ttttAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaacc                                                                                                       <  1:235054‑M1/251‑72 (MQ=255)                                                                                                                                     gTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtt                                                                                                                                                                >  1:502241‑M1/1‑175 (MQ=255)                                                                                                                                     gTCGTAGCCTCTCTTAGGTCATGGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgt                                                                                                                                                             <  1:433360‑M1/192‑18 (MQ=255)                                                                                                                                       cGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcg                                                                                                <  1:178130‑M1/251‑79 (MQ=255)                                                                                                                                           gCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaacc                                                                                                       <  1:674694‑M1/240‑72 (MQ=255)                                                                                                                                               ctctTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGatta                                                                                                                                                                               >  1:232937/1‑164 (MQ=255)                                                                                                                                               ctctTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaac                                                                                                        >  1:237051‑M1/1‑165 (MQ=255)                                                                                                                                               ctctTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATtta                                                                                                                                                                           >  1:286945‑M1/1‑165 (MQ=255)                                                                                                                                               ctctTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATtta                                                                                                                                                                           <  1:159570‑M1/168‑4 (MQ=255)                                                                                                                                                   tAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaa                                                                                    <  1:10872‑M1/251‑91 (MQ=255)                                                                                                                                                          ttGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaa                                                                             >  1:774508‑M1/1‑154 (MQ=255)                                                                                                                                                                    gTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACg                                                                                                                                                                                                                                                    >  1:419966/1‑74 (MQ=255)                                                                                                                                                                    gTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatg                                                                                                                      <  1:562842‑M1/200‑57 (MQ=255)                                                                                                                                                                     tGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttc                                                                     <  1:399151‑M1/248‑106 (MQ=255)                                                                                                                                                                      gCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgc                                                                                                            <  1:471454‑M1/208‑67 (MQ=255)                                                                                                                                                                           gTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTCTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattca                                                            >  1:212368‑M1/1‑137 (MQ=255)                                                                                                                                                                            tCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcat                                                           <  1:497831‑M1/251‑116 (MQ=255)                                                                                                                                                                             cTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGCAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcatt                                                          >  1:232269‑M1/1‑135 (MQ=255)                                                                                                                                                                                cAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATTTattattattg                                                                                                                                                                  <  1:41512/144‑5 (MQ=255)                                                                                                                                                                                    gACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcattttttac                                                    <  1:623529‑M1/250‑123 (MQ=255)                                                                                                                                                                                                       atgtaatcATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcattttttacccttaattaaattttaacaa                                >  1:12302‑M1/4‑109 (MQ=255)                                                                                                                                                                                                       atgtaatcATCTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaa                                                                >  1:789563‑M1/4‑109 (MQ=255)                                                                                                                                                                                                              catcTGCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaa                                                                <  1:565284‑M1/212‑111 (MQ=255)                                                                                                                                                                                                                   gCCTGTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatg                                                                                                                      >  1:278403‑M1/1‑97 (MQ=255)                                                                                                                                                                                                                       gTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcattttttacccttaattaaattttaacaaagtaaagtttccgct                 >  1:707386‑M1/1‑93 (MQ=255)                                                                                                                                                                                                                       gTAATAAATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattc                                                             <  1:578539‑M1/206‑114 (MQ=255)                                                                                                                                                                                                                          ataaATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccg                                                                                                      >  1:186333‑M1/1‑90 (MQ=255)                                                                                                                                                                                                                           taaATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaggcactgaaatgatcaagagcgttctgtaattcattttttacccttaattaaattttaacaaagtaaagtttccgctcaat             >  1:436300‑M1/1‑89 (MQ=255)                                                                                                                                                                                                                           taaATGACCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatg                                                                                                                      >  1:883051‑M1/1‑89 (MQ=255)                                                                                                                                                                                                                                 aCCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcattttttacccttaattaaattttaacaaagtaaagtttccgctcaatcaaaatt      <  1:545283‑M1/251‑169 (MQ=255)                                                                                                                                                                                                                                 aCCTTTGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcattttttacccttaattaaattttaacaaagtaaagtttccgctcaatcaaaat       <  1:166806‑M1/250‑168 (MQ=255)                                                                                                                                                                                                                                     ttGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaatgatcaagagcgttctgtaattcattttttacccttaattaaattttaacaaagtaaagtttccgctcaatcaaaattaatt  <  1:750939‑M1/251‑173 (MQ=255)                                                                                                                                                                                                                                     ttGCTCACGTCATTCTTTAATTCGGCAGTTAAAATTAGCTTTTCCGGCTTTTTCTCAGTTTCCGGAACCGACTGATTATttattattattgtttgttgattttcagtattgtcaaatgctggcataaacataatgccaccaatgccaaccgctagcgtaagcactgaaa                                                                                    >  1:460218‑M1/1‑79 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      TGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACTAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATATAATCATCTGCCTGTAATAAATGACCTTTGCTTACGTCATTCTTTAACTCGGCAGTTAAAATTAGCTTTTCCGGTTTTTTCTCAGCTTCCGGAACCTGCTGATTATTTATTATTGTTTGTTGATTTTCAGTATTGTCAAATGTTGGCATAAACATAATGCCACCAATGCCAACCGCTAGCGTAAGCACTGAAATGATCAAGAGCGTTCTGTAATTCATTTTTTACCCTTAATTAAATTTTAACAAAGTAAAGTCTCCGCTCAATCAAAATTAATTTGA  >  1/860046‑860524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑