Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	860,039	A→G	intergenic (‑10382/‑62179)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	860,039	0	A	G	100.0%	218.4 / NA	80	intergenic (‑10382/‑62179)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/42);  total (38/42)

TCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACTAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATATAATCATCTGCCTGTAATAAATGACCTTTGCTT  >  1/859830‑860279                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ttctcagtttcagtgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAgg                                                                                                                                                                                                         <  1:167761/236‑1 (MQ=255)              tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATGGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                                                                                                                              <  1:54289/247‑1 (MQ=255)              tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                                                                                                                              <  1:91141/247‑1 (MQ=255)               gccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgagaga                                                                                                                                                                                           >  1:740715/2‑251 (MQ=255)                    gttctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGataata                                                                                                                                                                                      >  1:47873/26‑251 (MQ=255)                       ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGa                                                                                                                                                                                    <  1:154701/228‑1 (MQ=255)                       ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTGGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAt                                                                                                                                                                                   <  1:87582/229‑1 (MQ=255)                                     aaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGaaa                                                                                                                                                                     <  1:564018/243‑1 (MQ=255)                                          ccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAc                                                                                                                                                                <  1:682175/248‑1 (MQ=255)                                           cgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                                                                                                                               <  1:412097/249‑1 (MQ=255)                                               cttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                                                                                                                               <  1:141345/247‑1 (MQ=255)                                                      aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAtt                                                                                                                                                                                  <  1:716179/211‑1 (MQ=255)                                                      aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACAt                                                                                                                                                    <  1:605186/241‑1 (MQ=255)                                                       accactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAg                                                                                                                                                                                                                 <  1:639918/180‑1 (MQ=255)                                                             gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTg                                                                                                                                              >  1:366099/4‑250 (MQ=255)                                                             gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATAAGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATttgttg                                                                                                                                                                             >  1:841199/4‑219 (MQ=255)                                                                    cTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTaaa                                                                                                                                      >  1:35820/1‑251 (MQ=255)                                                                     tAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAAt                                                                                                                                     <  1:105407/251‑1 (MQ=255)                                                                       aaactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAg                                                                                                                                   <  1:263498/247‑1 (MQ=255)                                                                        aactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGc                                                                                                                                  <  1:283197/248‑1 (MQ=255)                                                                              aaCTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCt                                                                                                                                >  1:8770/1‑247 (MQ=255)                                                                                cTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAAt                                                                                                                          <  1:553043/251‑1 (MQ=255)                                                                                     tCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATcc                                                                                                                        >  1:667185/1‑248 (MQ=255)                                                                                          aCGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATAc                                                                                                                <  1:218883/251‑1 (MQ=255)                                                                                             aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt                                                                                                             >  1:626647/1‑251 (MQ=255)                                                                                             aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt                                                                                                             >  1:10668/1‑251 (MQ=255)                                                                                             aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAAAGATAATAGATTTGTTGGATAGAAAAg                                                                                                                                                                   >  1:432130/1‑197 (MQ=255)                                                                                               cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATccc                                                                                                                       >  1:209340/1‑239 (MQ=255)                                                                                               cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttt                                                                                                           >  1:98335/1‑251 (MQ=255)                                                                                                    gtattGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt                                                                                                             <  1:187440/241‑1 (MQ=255)                                                                                                    gtattGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGt                                                                                                      >  1:769804/4‑251 (MQ=255)                                                                                                                 cTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCtt                                                                                         <  1:125742/251‑1 (MQ=255)                                                                                                                       cggtttgctggctataaacagataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGt                                                                                     <  1:144065/226‑1 (MQ=255)                                                                                                                                ggctataaacagataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGAc                                                                           >  1:447034/15‑250 (MQ=255)                                                                                                                                 gctataaacagataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTc                                                                                    >  1:840304/14‑240 (MQ=255)                                                                                                                                 gctataaacagataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGAcc                                                                          <  1:544388/237‑2 (MQ=255)                                                                                                                                 gctataaacagataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCCTAGGTCATTGTCGAcc                                                                          <  1:111620/237‑2 (MQ=255)                                                                                                                                       aacagataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCa                                                                    >  1:813078/8‑250 (MQ=255)                                                                                                                                           gataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGa                                                                                                                                                                                    <  1:85459/131‑1 (MQ=255)                                                                                                                                           gataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGcc                                                                                                                                 >  1:550103/4‑185 (MQ=255)                                                                                                                                           gataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttt                                                                                                           <  1:297848/204‑1 (MQ=255)                                                                                                                                           gataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCa                                                                    >  1:119626/4‑246 (MQ=255)                                                                                                                                           gataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCa                                                                    >  1:306997/4‑246 (MQ=255)                                                                                                                                           gataCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCa                                                                    >  1:429730/4‑246 (MQ=255)                                                                                                                                             taCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTaa                                                                                                                           <  1:80914/188‑1 (MQ=255)                                                                                                                                              aCATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTc                                                            <  1:375888/251‑1 (MQ=255)                                                                                                                                               cATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCa                                                           >  1:193416/1‑251 (MQ=255)                                                                                                                                                 tAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAgg                                                         <  1:13135/251‑1 (MQ=255)                                                                                                                                                   gTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttt                                                                                                           >  1:326446/1‑199 (MQ=255)                                                                                                                                                    tctcCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAggg                                                        <  1:699484/249‑1 (MQ=255)                                                                                                                                                        cgcTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGaa                                                  <  1:682349/249‑1 (MQ=255)                                                                                                                                                         gcTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGAt                                                                                                                                                                                                       >  1:344984/2‑101 (MQ=255)                                                                                                                                                         gcTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGa                                                                                                                                                                                    >  1:139195/2‑120 (MQ=255)                                                                                                                                                         gcTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGt                                                                                                      >  1:873273/2‑198 (MQ=255)                                                                                                                                                         gcTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGaaa                                                 >  1:114208/2‑251 (MQ=255)                                                                                                                                                         gcTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGaaa                                                 >  1:306672/2‑251 (MQ=255)                                                                                                                                                          cTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAAt                                                <  1:855304/251‑1 (MQ=255)                                                                                                                                                              aaaTAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCAc                                                                   >  1:83111/1‑228 (MQ=255)                                                                                                                                                                aTAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                                                                                                                               >  1:838395/1‑134 (MQ=255)                                                                                                                                                                aTAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAgg                                                                                      <  1:530847/207‑1 (MQ=255)                                                                                                                                                                   gTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAg                                                                                                                                   >  1:541637/1‑159 (MQ=255)                                                                                                                                                                   gTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAg                                                                                                       >  1:186589/1‑187 (MQ=255)                                                                                                                                                                   gTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAgg                                                         <  1:343120/233‑1 (MQ=255)                                                                                                                                                                     aTTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTaaaa                                     <  1:568385/251‑1 (MQ=255)                                                                                                                                                                           aaTGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAat                               <  1:348346/251‑1 (MQ=255)                                                                                                                                                                            aTGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGt                                                                                     <  1:764722/196‑1 (MQ=255)                                                                                                                                                                             tGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAatca                             <  1:736366/251‑1 (MQ=255)                                                                                                                                                                                     tcgccAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAgg                                                         >  1:827479/4‑215 (MQ=255)                                                                                                                                                                                           gatctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttttt                                                                                                         >  1:235949/5‑161 (MQ=255)                                                                                                                                                                                           gatctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGt                                                                                     >  1:609305/5‑181 (MQ=255)                                                                                                                                                                                           gatctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGt                                                                                     <  1:461257/177‑1 (MQ=255)                                                                                                                                                                                            atctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                                                                                                                               >  1:73119/4‑106 (MQ=255)                                                                                                                                                                                            atctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAc                                                                                                                                                                >  1:759656/4‑105 (MQ=255)                                                                                                                                                                                            atctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCAc                                                                   <  1:54393/195‑1 (MQ=255)                                                                                                                                                                                            atctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAgg                                                         >  1:590336/4‑208 (MQ=255)                                                                                                                                                                                            atctAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGtaat                 <  1:761314/245‑1 (MQ=255)                                                                                                                                                                                                      gATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTg     >  1:296670/1‑250 (MQ=255)                                                                                                                                                                                                       aTGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCt   <  1:711836/251‑1 (MQ=255)                                                                                                                                                                                                        tGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCtc  <  1:126383/251‑2 (MQ=255)                                                                                                                                                                                                        tGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACCAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATGTAATCATCTGCCTGTAATAAATGACCTTTGCtc  <  1:681385/251‑2 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 TCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTTAGGTCATTGTCGACTAGTGCACTGTCTTCAGGGACAGAAATCTCATTTAAAATATAATCATCTGCCTGTAATAAATGACCTTTGCTT  >  1/859830‑860279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑