Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,955	T→C	intergenic (‑10298/‑62263)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,955	0	T	C	100.0%	138.4 / NA	49	intergenic (‑10298/‑62263)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/30);  total (19/30)

ATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTT  >  1/859713‑860192                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                                                                                                                                                                                                                    <  1:464845/248‑4 (MQ=255) atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                                                                                                                                                                                                                    <  1:877339/248‑4 (MQ=255)       aactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatac                                                                                                                                                                                                                              <  1:606079/251‑10 (MQ=255)         cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                                                                                                                                                                                                                            <  1:780671/247‑12 (MQ=255)         cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                                                                                                                                                                                                                            >  1:551970/5‑240 (MQ=255)           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                                          <  1:894938/249‑14 (MQ=255)           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                                          <  1:773401/249‑14 (MQ=255)           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                                          <  1:62278/249‑14 (MQ=255)           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                                          <  1:479787/249‑14 (MQ=255)                tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTACAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGtctc                                                                                                                                                                                                                      >  1:453155/1‑250 (MQ=255)                 ctctATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTccg                                                                                                                                                                                                                    >  1:885580/1‑250 (MQ=255)                     aTCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                                          >  1:459579/1‑228 (MQ=255)                           tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt                                                                                                                                                                                                            <  1:454188/249‑1 (MQ=255)                           tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt                                                                                                                                                                                                            <  1:448427/249‑1 (MQ=255)                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatagtc                                                                                                                                                                                                                        <  1:279359/231‑16 (MQ=255)                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa                                                                                                                                                                                                    >  1:2960/1‑251 (MQ=255)                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa                                                                                                                                                                                                    >  1:543537/1‑251 (MQ=255)                                                                                                                       ttctcagtttcagtgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAgg                                                                                                                  <  1:167761/236‑1 (MQ=255)                                                                                                                                    tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATGGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                                       <  1:54289/247‑1 (MQ=255)                                                                                                                                    tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                                       <  1:91141/247‑1 (MQ=255)                                                                                                                                     gccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgagaga                                                                                                    >  1:740715/2‑251 (MQ=255)                                                                                                                                          gttctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGataata                                                                                               >  1:47873/26‑251 (MQ=255)                                                                                                                                             ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGa                                                                                             <  1:154701/228‑1 (MQ=255)                                                                                                                                             ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTGGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAt                                                                                            <  1:87582/229‑1 (MQ=255)                                                                                                                                                           aaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGaaa                                                                              <  1:564018/243‑1 (MQ=255)                                                                                                                                                                ccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAc                                                                         <  1:682175/248‑1 (MQ=255)                                                                                                                                                                 cgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                                        <  1:412097/249‑1 (MQ=255)                                                                                                                                                                     cttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                                        <  1:141345/247‑1 (MQ=255)                                                                                                                                                                            aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAtt                                                                                           <  1:716179/211‑1 (MQ=255)                                                                                                                                                                            aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACAt                                                             <  1:605186/241‑1 (MQ=255)                                                                                                                                                                             accactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAg                                                                                                                          <  1:639918/180‑1 (MQ=255)                                                                                                                                                                                   gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTg                                                       >  1:366099/4‑250 (MQ=255)                                                                                                                                                                                   gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATAAGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATttgttg                                                                                      >  1:841199/4‑219 (MQ=255)                                                                                                                                                                                          cTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTaaa                                               >  1:35820/1‑251 (MQ=255)                                                                                                                                                                                           tAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAAt                                              <  1:105407/251‑1 (MQ=255)                                                                                                                                                                                             aaactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAg                                            <  1:263498/247‑1 (MQ=255)                                                                                                                                                                                              aactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGc                                           <  1:283197/248‑1 (MQ=255)                                                                                                                                                                                                    aaCTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCt                                         >  1:8770/1‑247 (MQ=255)                                                                                                                                                                                                      cTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAAt                                   <  1:553043/251‑1 (MQ=255)                                                                                                                                                                                                           tCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATcc                                 >  1:667185/1‑248 (MQ=255)                                                                                                                                                                                                                aCGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATAc                         <  1:218883/251‑1 (MQ=255)                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt                      >  1:10668/1‑251 (MQ=255)                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt                      >  1:626647/1‑251 (MQ=255)                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAAAGATAATAGATTTGTTGGATAGAAAAg                                                                            >  1:432130/1‑197 (MQ=255)                                                                                                                                                                                                                     cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATccc                                >  1:209340/1‑239 (MQ=255)                                                                                                                                                                                                                     cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttt                    >  1:98335/1‑251 (MQ=255)                                                                                                                                                                                                                          gtattGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt                      <  1:187440/241‑1 (MQ=255)                                                                                                                                                                                                                          gtattGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGt               >  1:769804/4‑251 (MQ=255)                                                                                                                                                                                                                                       cTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCtt  <  1:125742/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              ATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGTCGTAGCCTCTCTT  >  1/859713‑860192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑