Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,937	C→G	intergenic (‑10280/‑62281)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,937	0	C	G	100.0%	137.2 / NA	50	intergenic (‑10280/‑62281)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/30);  total (20/30)

ATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGT  >  1/859697‑860179                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   attTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcgg                                                                                                                                                                                                                                       >  1:725284/1‑249 (MQ=255)                 atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                                                                                                                                                                                                       <  1:464845/248‑4 (MQ=255)                 atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                                                                                                                                                                                                       <  1:877339/248‑4 (MQ=255)                       aactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatac                                                                                                                                                                                                                 <  1:606079/251‑10 (MQ=255)                         cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                                                                                                                                                                                                               <  1:780671/247‑12 (MQ=255)                         cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                                                                                                                                                                                                               >  1:551970/5‑240 (MQ=255)                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                             <  1:894938/249‑14 (MQ=255)                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                             <  1:479787/249‑14 (MQ=255)                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                             <  1:62278/249‑14 (MQ=255)                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                             <  1:773401/249‑14 (MQ=255)                                tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTACAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGtctc                                                                                                                                                                                                         >  1:453155/1‑250 (MQ=255)                                tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcggtttgct                                                                                                                                                                                                                                 <  1:445055/226‑9 (MQ=255)                                 ctctATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTccg                                                                                                                                                                                                       >  1:885580/1‑250 (MQ=255)                                     aTCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                             >  1:459579/1‑228 (MQ=255)                                           tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt                                                                                                                                                                                               <  1:454188/249‑1 (MQ=255)                                           tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt                                                                                                                                                                                               <  1:448427/249‑1 (MQ=255)                                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatagtc                                                                                                                                                                                                           <  1:279359/231‑16 (MQ=255)                                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa                                                                                                                                                                                       >  1:543537/1‑251 (MQ=255)                                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa                                                                                                                                                                                       >  1:2960/1‑251 (MQ=255)                                                                                                                                       ttctcagtttcagtgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAgg                                                                                                     <  1:167761/236‑1 (MQ=255)                                                                                                                                                    tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATGGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                          <  1:54289/247‑1 (MQ=255)                                                                                                                                                    tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                          <  1:91141/247‑1 (MQ=255)                                                                                                                                                     gccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgagaga                                                                                       >  1:740715/2‑251 (MQ=255)                                                                                                                                                          gttctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGataata                                                                                  >  1:47873/26‑251 (MQ=255)                                                                                                                                                             ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGa                                                                                <  1:154701/228‑1 (MQ=255)                                                                                                                                                             ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTGGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAt                                                                               <  1:87582/229‑1 (MQ=255)                                                                                                                                                                           aaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGaaa                                                                 <  1:564018/243‑1 (MQ=255)                                                                                                                                                                                ccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAc                                                            <  1:682175/248‑1 (MQ=255)                                                                                                                                                                                 cgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                           <  1:412097/249‑1 (MQ=255)                                                                                                                                                                                     cttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                           <  1:141345/247‑1 (MQ=255)                                                                                                                                                                                            aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAtt                                                                              <  1:716179/211‑1 (MQ=255)                                                                                                                                                                                            aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACAt                                                <  1:605186/241‑1 (MQ=255)                                                                                                                                                                                             accactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAg                                                                                                             <  1:639918/180‑1 (MQ=255)                                                                                                                                                                                                   gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTg                                          >  1:366099/4‑250 (MQ=255)                                                                                                                                                                                                   gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATAAGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATttgttg                                                                         >  1:841199/4‑219 (MQ=255)                                                                                                                                                                                                          cTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTaaa                                  >  1:35820/1‑251 (MQ=255)                                                                                                                                                                                                           tAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAAt                                 <  1:105407/251‑1 (MQ=255)                                                                                                                                                                                                             aaactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAg                               <  1:263498/247‑1 (MQ=255)                                                                                                                                                                                                              aactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGc                              <  1:283197/248‑1 (MQ=255)                                                                                                                                                                                                                    aaCTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCt                            >  1:8770/1‑247 (MQ=255)                                                                                                                                                                                                                      cTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAAt                      <  1:553043/251‑1 (MQ=255)                                                                                                                                                                                                                           tCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATcc                    >  1:667185/1‑248 (MQ=255)                                                                                                                                                                                                                                aCGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATAc            <  1:218883/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt         >  1:10668/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt         >  1:626647/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAAAGATAATAGATTTGTTGGATAGAAAAg                                                               >  1:432130/1‑197 (MQ=255)                                                                                                                                                                                                                                     cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATccc                   >  1:209340/1‑239 (MQ=255)                                                                                                                                                                                                                                     cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttt       >  1:98335/1‑251 (MQ=255)                                                                                                                                                                                                                                          gtattGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt         <  1:187440/241‑1 (MQ=255)                                                                                                                                                                                                                                          gtattGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGt  >  1:769804/4‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   ATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTTTTAGT  >  1/859697‑860179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑