Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,931	G→A	intergenic (‑10274/‑62287)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,931	0	G	A	100.0%	138.5 / NA	50	intergenic (‑10274/‑62287)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/31);  total (19/31)

CAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTT  >  1/859681‑860174                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    cAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTg                                                                                                                                                                                                                                                  <  1:410931/251‑1 (MQ=255)                 attTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcgg                                                                                                                                                                                                                                  >  1:725284/1‑249 (MQ=255)                                 atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                                                                                                                                                                                                  <  1:877339/248‑4 (MQ=255)                                 atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                                                                                                                                                                                                  <  1:464845/248‑4 (MQ=255)                                       aactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatac                                                                                                                                                                                                            <  1:606079/251‑10 (MQ=255)                                         cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                                                                                                                                                                                                          <  1:780671/247‑12 (MQ=255)                                         cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                                                                                                                                                                                                          >  1:551970/5‑240 (MQ=255)                                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                        <  1:479787/249‑14 (MQ=255)                                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                        <  1:773401/249‑14 (MQ=255)                                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                        <  1:62278/249‑14 (MQ=255)                                           tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                        <  1:894938/249‑14 (MQ=255)                                                tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTACAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGtctc                                                                                                                                                                                                    >  1:453155/1‑250 (MQ=255)                                                tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcggtttgct                                                                                                                                                                                                                            <  1:445055/226‑9 (MQ=255)                                                 ctctATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTccg                                                                                                                                                                                                  >  1:885580/1‑250 (MQ=255)                                                     aTCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                                                                                                                                                                                                        >  1:459579/1‑228 (MQ=255)                                                           tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt                                                                                                                                                                                          <  1:454188/249‑1 (MQ=255)                                                           tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt                                                                                                                                                                                          <  1:448427/249‑1 (MQ=255)                                                            gAATAGAATTTTTTAGCTTTTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTgcggg                                                                                                                                                                                                                                              <  1:63357/196‑3 (MQ=255)                                                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatagtc                                                                                                                                                                                                      <  1:279359/231‑16 (MQ=255)                                                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa                                                                                                                                                                                  >  1:2960/1‑251 (MQ=255)                                                                 gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa                                                                                                                                                                                  >  1:543537/1‑251 (MQ=255)                                                                                                                                                       ttctcagtttcagtgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAgg                                                                                                <  1:167761/236‑1 (MQ=255)                                                                                                                                                                    tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATGGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                     <  1:54289/247‑1 (MQ=255)                                                                                                                                                                    tgccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgag                                                                                     <  1:91141/247‑1 (MQ=255)                                                                                                                                                                     gccTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGgagaga                                                                                  >  1:740715/2‑251 (MQ=255)                                                                                                                                                                          gttctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGataata                                                                             >  1:47873/26‑251 (MQ=255)                                                                                                                                                                             ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGa                                                                           <  1:154701/228‑1 (MQ=255)                                                                                                                                                                             ctgctacttgagcaaaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTGGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAt                                                                          <  1:87582/229‑1 (MQ=255)                                                                                                                                                                                           aaattccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGaaa                                                            <  1:564018/243‑1 (MQ=255)                                                                                                                                                                                                ccgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAc                                                       <  1:682175/248‑1 (MQ=255)                                                                                                                                                                                                 cgtgcttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                      <  1:412097/249‑1 (MQ=255)                                                                                                                                                                                                     cttgTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAAcc                                                      <  1:141345/247‑1 (MQ=255)                                                                                                                                                                                                            aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGAtt                                                                         <  1:716179/211‑1 (MQ=255)                                                                                                                                                                                                            aaccactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACAt                                           <  1:605186/241‑1 (MQ=255)                                                                                                                                                                                                             accactgccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAg                                                                                                        <  1:639918/180‑1 (MQ=255)                                                                                                                                                                                                                   gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTg                                     >  1:366099/4‑250 (MQ=255)                                                                                                                                                                                                                   gccttACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATAAGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATttgttg                                                                    >  1:841199/4‑219 (MQ=255)                                                                                                                                                                                                                          cTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTaaa                             >  1:35820/1‑251 (MQ=255)                                                                                                                                                                                                                           tAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAAt                            <  1:105407/251‑1 (MQ=255)                                                                                                                                                                                                                             aaactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAg                          <  1:263498/247‑1 (MQ=255)                                                                                                                                                                                                                              aactttAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGc                         <  1:283197/248‑1 (MQ=255)                                                                                                                                                                                                                                    aaCTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCt                       >  1:8770/1‑247 (MQ=255)                                                                                                                                                                                                                                      cTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAAt                 <  1:553043/251‑1 (MQ=255)                                                                                                                                                                                                                                           tCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATcc               >  1:667185/1‑248 (MQ=255)                                                                                                                                                                                                                                                aCGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATAc       <  1:218883/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt    >  1:10668/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAt    >  1:626647/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aTCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAAAGATAATAGATTTGTTGGATAGAAAAg                                                          >  1:432130/1‑197 (MQ=255)                                                                                                                                                                                                                                                     cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATccc              >  1:209340/1‑239 (MQ=255)                                                                                                                                                                                                                                                     cATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTAGAATGTAATCATCGCCAGATCTAACATAGATGCGATATGCGACTTCTTGACTTGGATTCAAGCTGGAAGTAAAGAATCTAGGATCATTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCAttt  >  1:98335/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    CAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTAGAATGTAATCATCACCAGATTTAACATAGATGCGATATGCAACTTCTTGATTCGGATCTAGGCTGGATGTAAAGAATCTAGGATCGTTCGGAGAGATAATAGATTTGTTGGATAGAAAAGAACCAGCTCTGACATTTTCTGCCATTAAATAGCCTTGTAATCCCTGAATACCATTT  >  1/859681‑860174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑