Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,797	T→A	intergenic (‑10140/‑62421)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,797	0	T	A	100.0%	84.4 / NA	32	intergenic (‑10140/‑62421)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/21);  total (11/21)

CGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCATATTAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTA  >  1/859554‑859998                                                                                                                                                                                                                                                    |                                                                                                                                                                                                           cGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcac                                                                                                                                                                                                      <  1:269798/250‑4 (MQ=255)   tACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactat                                                                                                                                                                                                   >  1:92237/1‑245 (MQ=255)       cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttgg                                                                                                                                                                                               >  1:695876/1‑241 (MQ=255)          ttAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagc                                                                                                                                                                                            <  1:155145/251‑14 (MQ=255)          ttAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagc                                                                                                                                                                                            <  1:75646/251‑14 (MQ=255)                      ttGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagccagacctaacgc                                                                                                                                                                                <  1:77006/251‑26 (MQ=255)                                  cTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactattt                                                                                                                                                                                                 >  1:68651/1‑214 (MQ=255)                                                                                                 ctTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcac                                                                                                                                                                                                      <  1:123365/154‑4 (MQ=255)                                                                                                                           tGAATCAACTTCTTGAGTATTATTTTCGACAGGCAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAg                                                                       <  1:108817/251‑1 (MQ=255)                                                                                                                                cAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTg                                                                  <  1:410931/251‑1 (MQ=255)                                                                                                                                                attTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcgg                                                  >  1:725284/1‑249 (MQ=255)                                                                                                                                                                atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                  <  1:877339/248‑4 (MQ=255)                                                                                                                                                                atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                  <  1:464845/248‑4 (MQ=255)                                                                                                                                                                      aactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatac                            <  1:606079/251‑10 (MQ=255)                                                                                                                                                                        cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                          <  1:780671/247‑12 (MQ=255)                                                                                                                                                                        cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                          >  1:551970/5‑240 (MQ=255)                                                                                                                                                                          tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:894938/249‑14 (MQ=255)                                                                                                                                                                          tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:479787/249‑14 (MQ=255)                                                                                                                                                                          tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:62278/249‑14 (MQ=255)                                                                                                                                                                          tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:773401/249‑14 (MQ=255)                                                                                                                                                                             aTTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAgtat                                                                    >  1:857967/1‑202 (MQ=255)                                                                                                                                                                               tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTACAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGtctc                    >  1:453155/1‑250 (MQ=255)                                                                                                                                                                               tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcggtttgct                                            <  1:445055/226‑9 (MQ=255)                                                                                                                                                                                ctctATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTccg                  >  1:885580/1‑250 (MQ=255)                                                                                                                                                                                ctctATCCAGTGAATAGAATTTTTTAGCTCGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAgt                                                                      <  1:454210/199‑1 (MQ=255)                                                                                                                                                                                    aTCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        >  1:459579/1‑228 (MQ=255)                                                                                                                                                                                          tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt          <  1:454188/249‑1 (MQ=255)                                                                                                                                                                                          tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt          <  1:448427/249‑1 (MQ=255)                                                                                                                                                                                           gAATAGAATTTTTTAGCTTTTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTgcggg                                                              <  1:63357/196‑3 (MQ=255)                                                                                                                                                                                                gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatagtc                      <  1:279359/231‑16 (MQ=255)                                                                                                                                                                                                gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa  >  1:2960/1‑251 (MQ=255)                                                                                                                                                                                                gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa  >  1:543537/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                           CGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCATATTAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTA  >  1/859554‑859998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑