Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,777	G→A	intergenic (‑10120/‑62441)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,777	0	G	A	100.0%	138.7 / NA	52	intergenic (‑10120/‑62441)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/33);  total (19/33)

CTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCATATTAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTA  >  1/859530‑859998                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               cTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGc                                                                                                                                                                                                                             <  1:83593/251‑1 (MQ=255) cTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTGGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGc                                                                                                                                                                                                                             <  1:444036/251‑1 (MQ=255)    tATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTa                                                                                                                                                                                                                           >  1:276263/1‑250 (MQ=255)      tCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACAt                                                                                                                                                                                                                        <  1:618767/251‑1 (MQ=255)      tCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACAt                                                                                                                                                                                                                        <  1:632705/251‑1 (MQ=255)                   tttGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatac                                                                                                                                                                                                           <  1:484353/251‑3 (MQ=255)                         cGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcac                                                                                                                                                                                                      <  1:269798/250‑4 (MQ=255)                           tACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactat                                                                                                                                                                                                   >  1:92237/1‑245 (MQ=255)                               cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                                                                     <  1:336556/229‑1 (MQ=255)                               cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttgg                                                                                                                                                                                               >  1:695876/1‑241 (MQ=255)                                  ttAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagc                                                                                                                                                                                            <  1:75646/251‑14 (MQ=255)                                  ttAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagc                                                                                                                                                                                            <  1:155145/251‑14 (MQ=255)                                    aaTGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                                                                     >  1:330657/1‑224 (MQ=255)                                         gaACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                                                                     <  1:225774/219‑1 (MQ=255)                                         gaACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatat                                                                                                                                                                                                             <  1:625327/227‑1 (MQ=255)                                              ttGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagccagacctaacgc                                                                                                                                                                                <  1:77006/251‑26 (MQ=255)                                                          cTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                                                                     >  1:690772/1‑202 (MQ=255)                                                          cTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactattt                                                                                                                                                                                                 >  1:68651/1‑214 (MQ=255)                                                                 gCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatac                                                                                                                                                                                                           <  1:806659/205‑3 (MQ=255)                                                                            gTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                                                                     >  1:56953/1‑184 (MQ=255)                                                                                        aaGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAccg                                                                                                                                                                                                                   <  1:797297/174‑2 (MQ=255)                                                                                                            aTCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACAt                                                                                                                                                                                                                        <  1:146973/149‑1 (MQ=255)                                                                                                                         ctTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcac                                                                                                                                                                                                      <  1:123365/154‑4 (MQ=255)                                                                                                                                    gatgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatac                                                                                                                                                                                                           >  1:61838/1‑136 (MQ=255)                                                                                                                                      tgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAccggc                                                                                                                                                                                                                 >  1:511361/1‑127 (MQ=255)                                                                                                                                      tgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatact                                                                                                                                                                                                          >  1:238118/1‑134 (MQ=255)                                                                                                                                      tgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatact                                                                                                                                                                                                          >  1:421842/1‑134 (MQ=255)                                                                                                                                                   tGAATCAACTTCTTGAGTATTATTTTCGACAGGCAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAg                                                                       <  1:108817/251‑1 (MQ=255)                                                                                                                                                     aaTCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                                                                     <  1:818847/111‑1 (MQ=255)                                                                                                                                                        cAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTg                                                                  <  1:410931/251‑1 (MQ=255)                                                                                                                                                                        attTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcgg                                                  >  1:725284/1‑249 (MQ=255)                                                                                                                                                                                        atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                  <  1:464845/248‑4 (MQ=255)                                                                                                                                                                                        atgactaactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaac                                  <  1:877339/248‑4 (MQ=255)                                                                                                                                                                                              aactTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatac                            <  1:606079/251‑10 (MQ=255)                                                                                                                                                                                                cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                          <  1:780671/247‑12 (MQ=255)                                                                                                                                                                                                cttcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacat                          >  1:551970/5‑240 (MQ=255)                                                                                                                                                                                                  tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:479787/249‑14 (MQ=255)                                                                                                                                                                                                  tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:894938/249‑14 (MQ=255)                                                                                                                                                                                                  tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:773401/249‑14 (MQ=255)                                                                                                                                                                                                  tcgATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        <  1:62278/249‑14 (MQ=255)                                                                                                                                                                                                     aTTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAgtat                                                                    >  1:857967/1‑202 (MQ=255)                                                                                                                                                                                                       tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTACAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGtctc                    >  1:453155/1‑250 (MQ=255)                                                                                                                                                                                                       tctcTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcggtttgct                                            <  1:445055/226‑9 (MQ=255)                                                                                                                                                                                                        ctctATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTccg                  >  1:885580/1‑250 (MQ=255)                                                                                                                                                                                                        ctctATCCAGTGAATAGAATTTTTTAGCTCGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAgt                                                                      <  1:454210/199‑1 (MQ=255)                                                                                                                                                                                                            aTCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatag                        >  1:459579/1‑228 (MQ=255)                                                                                                                                                                                                                  tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt          <  1:448427/249‑1 (MQ=255)                                                                                                                                                                                                                  tGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGATCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAAt          <  1:454188/249‑1 (MQ=255)                                                                                                                                                                                                                   gAATAGAATTTTTTAGCTTTTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTgcggg                                                              <  1:63357/196‑3 (MQ=255)                                                                                                                                                                                                                        gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCtataaacagatacatagtc                      <  1:279359/231‑16 (MQ=255)                                                                                                                                                                                                                        gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa  >  1:2960/1‑251 (MQ=255)                                                                                                                                                                                                                        gAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGCGGTTTGCTGGCTATAAACAGATACATAGTCTCCGCTTAAAATAGTATTTa  >  1:543537/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               CTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCATATTAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAATTTGTTGGCTATAGATAGCAACATAGTCTCCACTTAAAATAGTATTTA  >  1/859530‑859998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑