Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,704	G→A	intergenic (‑10047/‑62514)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,704	0	G	A	100.0%	210.5 / NA	75	intergenic (‑10047/‑62514)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/39);  total (36/39)

GTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCATATTAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAA  >  1/859459‑859950                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        gTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGt                                                                                                                                                                                                                                                    >  1:820686/1‑251 (MQ=255)   cGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAGTTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTaa                                                                                                                                                                                                                                                  >  1:436534/1‑251 (MQ=255)       aaTATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAatatg                                                                                                                                                                                                                                              <  1:170415/251‑2 (MQ=255)         tatTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAatatga                                                                                                                                                                                                                                             >  1:569611/1‑248 (MQ=255)             tGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATAtgactaac                                                                                                                                                                                                                                        <  1:84226/251‑1 (MQ=255)                 gAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACttcg                                                                                                                                                                                                                                    >  1:212825/1‑249 (MQ=255)                      gaCGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGAtt                                                                                                                                                                                                                                 >  1:555335/1‑249 (MQ=255)                        cGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATtct                                                                                                                                                                                                                               >  1:651935/1‑249 (MQ=255)                           aaaTAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTAt                                                                                                                                                                                                                           >  1:517146/1‑250 (MQ=255)                            aaTAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATcc                                                                                                                                                                                                                         <  1:899871/251‑1 (MQ=255)                            aaTAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATcc                                                                                                                                                                                                                         <  1:305110/251‑1 (MQ=255)                            aaTAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATcc                                                                                                                                                                                                                         <  1:749514/251‑1 (MQ=255)                            aaTAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATcc                                                                                                                                                                                                                         <  1:269154/251‑1 (MQ=255)                            aaTAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATcc                                                                                                                                                                                                                         <  1:117825/251‑1 (MQ=255)                                 tgtCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGa                                                                                                                                                                                                                    <  1:756480/251‑1 (MQ=255)                                  gtCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGaa                                                                                                                                                                                                                   >  1:626351/1‑251 (MQ=255)                                        cttttcttttGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAAt                                                                                                                                                                                                             >  1:279379/1‑251 (MQ=255)                                        cttttcttttGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAAt                                                                                                                                                                                                             <  1:812985/251‑1 (MQ=255)                                             cttttGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAAtttttt                                                                                                                                                                                                        <  1:355778/251‑1 (MQ=255)                                             cttttGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAAtttttt                                                                                                                                                                                                        <  1:356604/251‑1 (MQ=255)                                                 tgtttgAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAatatg                                                                                                                                                                                                                                              >  1:835569/1‑208 (MQ=255)                                                 tgtttgAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCt                                                                                                                                                                                                    <  1:468089/251‑1 (MQ=255)                                                  gtttgAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCtt                                                                                                                                                                                                   >  1:398136/1‑251 (MQ=255)                                                         ccAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAAttttttagc                                                                                                                                                                                                     >  1:197768/1‑239 (MQ=255)                                                           aaCTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTcagcag                                                                                                                                                                                          <  1:881679/251‑1 (MQ=255)                                                           aaCTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTcagcag                                                                                                                                                                                          <  1:334324/251‑1 (MQ=255)                                                                ggTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAAttttttagc                                                                                                                                                                                                     <  1:876821/235‑4 (MQ=255)                                                                   tGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTAttttt                                                                                                                                                                                  <  1:39906/251‑1 (MQ=255)                                                                       ccTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTaatg                                                                                                                                                                              >  1:791841/1‑248 (MQ=255)                                                                       ccTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTaatg                                                                                                                                                                              >  1:20993/1‑248 (MQ=255)                                                                       ccTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTaatg                                                                                                                                                                              >  1:849852/1‑248 (MQ=255)                                                                       ccTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACAGTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTaatg                                                                                                                                                                              <  1:109342/251‑4 (MQ=255)                                                                        cTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGc                                                                                                                                                                             <  1:83593/251‑1 (MQ=255)                                                                        cTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTGGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGc                                                                                                                                                                             <  1:444036/251‑1 (MQ=255)                                                                           tATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTa                                                                                                                                                                           >  1:276263/1‑250 (MQ=255)                                                                             tCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACAt                                                                                                                                                                        <  1:632705/251‑1 (MQ=255)                                                                             tCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACAt                                                                                                                                                                        <  1:618767/251‑1 (MQ=255)                                                                                   gagaAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATtc                                                                                                                                                                                                                                >  1:539393/1‑189 (MQ=255)                                                                                          tttGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatac                                                                                                                                                           <  1:484353/251‑3 (MQ=255)                                                                                                cGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcac                                                                                                                                                      <  1:269798/250‑4 (MQ=255)                                                                                                  tACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactat                                                                                                                                                   >  1:92237/1‑245 (MQ=255)                                                                                                      cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATtc                                                                                                                                                                                                                                >  1:54644/1‑170 (MQ=255)                                                                                                      cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCt                                                                                                                                                                                                    >  1:270020/1‑198 (MQ=255)                                                                                                      cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                     <  1:336556/229‑1 (MQ=255)                                                                                                      cATTTAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttgg                                                                                                                                               >  1:695876/1‑241 (MQ=255)                                                                                                         ttAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagc                                                                                                                                            <  1:155145/251‑14 (MQ=255)                                                                                                         ttAATGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagc                                                                                                                                            <  1:75646/251‑14 (MQ=255)                                                                                                           aaTGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                     >  1:330657/1‑224 (MQ=255)                                                                                                             tGAGAACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATAtgactaac                                                                                                                                                                                                                                        >  1:877660/1‑155 (MQ=255)                                                                                                                gaACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTcagcag                                                                                                                                                                                          >  1:107458/1‑198 (MQ=255)                                                                                                                gaACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                     <  1:225774/219‑1 (MQ=255)                                                                                                                gaACATTGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatat                                                                                                                                                             <  1:625327/227‑1 (MQ=255)                                                                                                                     ttGCTCCGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactatttggagccagacctaacgc                                                                                                                                <  1:77006/251‑26 (MQ=255)                                                                                                                       gctccGAGAACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTcag                                                                                                                                                                                             >  1:602000/4‑188 (MQ=255)                                                                                                                              gaACTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTcagc                                                                                                                                                                                            <  1:836463/182‑1 (MQ=255)                                                                                                                                 cTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAatatg                                                                                                                                                                                                                                              >  1:577255/1‑128 (MQ=255)                                                                                                                                 cTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                     >  1:690772/1‑202 (MQ=255)                                                                                                                                 cTGCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcactattt                                                                                                                                                 >  1:68651/1‑214 (MQ=255)                                                                                                                                   gCACAGCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAatatg                                                                                                                                                                                                                                              <  1:438100/127‑2 (MQ=255)                                                                                                                                        gCATATCAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatac                                                                                                                                                           <  1:806659/205‑3 (MQ=255)                                                                                                                                           tatcaaTTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGA‑GAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACtt                                                                                                                                                                                                                                      >  1:559612/5‑126 (MQ=255)                                                                                                                                                   gTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                     >  1:56953/1‑184 (MQ=255)                                                                                                                                                              aaaGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTAttttt                                                                                                                                                                                  >  1:717180/1‑160 (MQ=255)                                                                                                                                                               aaGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAccg                                                                                                                                                                   <  1:797297/174‑2 (MQ=255)                                                                                                                                                                 gTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATcc                                                                                                                                                                                                                         <  1:54168/118‑1 (MQ=255)                                                                                                                                                                                   aTCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACAt                                                                                                                                                                        <  1:146973/149‑1 (MQ=255)                                                                                                                                                                                                ctTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACttcac                                                                                                                                                      <  1:123365/154‑4 (MQ=255)                                                                                                                                                                                                           gatgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatac                                                                                                                                                           >  1:61838/1‑136 (MQ=255)                                                                                                                                                                                                             tgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAccggc                                                                                                                                                                 >  1:511361/1‑127 (MQ=255)                                                                                                                                                                                                             tgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatact                                                                                                                                                          >  1:238118/1‑134 (MQ=255)                                                                                                                                                                                                             tgaATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCatatact                                                                                                                                                          >  1:421842/1‑134 (MQ=255)                                                                                                                                                                                                                          tGAATCAACTTCTTGAGTATTATTTTCGACAGGCAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAg                       <  1:108817/251‑1 (MQ=255)                                                                                                                                                                                                                            aaTCAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAAc                                                                                                                                                                     <  1:818847/111‑1 (MQ=255)                                                                                                                                                                                                                               cAACTTCTTGAGTATTATTTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTg                  <  1:410931/251‑1 (MQ=255)                                                                                                                                                                                                                                               attTTCGACAGGTAATATGACTAACTTCGATTCTCTATCCAGTGAATAGAATTTTTTAGCTTGTTCAGCAGTTATTTTTAATGCTACATAACCGGCATATACTTCACTATTTGG‑AGCCAGACCTAACGCTTTCTCAGTTTCA‑GTG‑‑CCTTGTTCTGCTACTTGAGCAAAATTCCGTGCTTGTAGAACCACTGCCTTACCTAAAACTTTAACTAAATCTTTACGATCATAAGTATTGCGGGAATCTGAAGcgg  >  1:725284/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        GTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCATATTAATTGTTTTTTGCCAAAAGTATGATTCCAATTTTGCATCTTATTGACCTCTTAATTTTCTGATGAATACACCGCGTGAATCAACTTCTTGAGTATTATTTTCGGCAGGTAATATAACTAACTTTGATTCTCTATCCAGTGAATAGAATTTTTTTGCTTGTTCAGCAGTTATTTTTAGTGCTACATAACCAGCATATTCTTGATTGTTTGGAATCCCG‑TCTGCTGCCTTTTCAGCTTCAGGAGCACCTTGTTCTGTTGATTGAGTAAAGTTTCTTGCTTGTAAAACCAATACTTTACCTAAAATTTTAACTAAATCTTTACGATCATAAGTGTTGCGCGAATCTGAAGCAA  >  1/859459‑859950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑