Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,360	G→A	intergenic (‑9703/‑62858)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,360	0	G	A	100.0%	178.5 / NA	68	intergenic (‑9703/‑62858)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/29);  total (39/29)

TTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCA  >  1/859156‑859596                                                                                                                                                                                                             |                                                                                                                                                                                                                                             ttCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTc                                                                                                                                                                                                <  1:818469/251‑1 (MQ=255)  tCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGtt                                                                                                                                                                                                 >  1:80305/1‑249 (MQ=255)      tAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTAc                                                                                                                                                                                             <  1:726356/249‑1 (MQ=255)                      tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTc                                                                                                                                                                                                     >  1:556071/1‑225 (MQ=255)                      tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGt                                                                                                                                                                                                  >  1:395681/1‑228 (MQ=255)                            aGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTg                                                                                                                                                                                 <  1:676765/239‑1 (MQ=255)                              cTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATaaa                                                                                                                                                                    <  1:703990/250‑1 (MQ=255)                                 ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                                                                                                                                                <  1:597552/251‑1 (MQ=255)                                 ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                                                                                                                                                <  1:605228/251‑1 (MQ=255)                                           tctacGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTa                                                                                                                                                      >  1:144644/5‑251 (MQ=255)                                                gAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                                                                                                                                                >  1:244589/1‑236 (MQ=255)                                                                      tgaACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTg                                                                                                                           <  1:75060/250‑1 (MQ=255)                                                                                  accttCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTtcctc                                                                                                                                                                                      >  1:224996/5‑180 (MQ=255)                                                                                       cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTg                                                                                                                                                                                                   >  1:455650/1‑162 (MQ=255)                                                                                       cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTAc                                                                                                                                                                                             <  1:519009/168‑1 (MQ=255)                                                                                       cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg                                                                                                          >  1:682816/1‑251 (MQ=255)                                                                                       cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg                                                                                                          >  1:873240/1‑251 (MQ=255)                                                                                       cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg                                                                                                          >  1:554590/1‑251 (MQ=255)                                                                                       cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg                                                                                                          >  1:639839/1‑251 (MQ=255)                                                                                          ttACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATaaa                                                                                                                                                                    <  1:337503/190‑1 (MQ=255)                                                                                            aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAAt                                                                                                              >  1:673619/1‑242 (MQ=255)                                                                                            aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt                                                                                                     >  1:737393/1‑251 (MQ=255)                                                                                            aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt                                                                                                     >  1:368532/1‑251 (MQ=255)                                                                                            aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt                                                                                                     >  1:580437/1‑251 (MQ=255)                                                                                            aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt                                                                                                     >  1:641929/1‑251 (MQ=255)                                                                                                 ggCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCt                                                                                                                                                                                               >  1:658551/2‑156 (MQ=255)                                                                                                 ggCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAatat                                                                                                                                 >  1:296150/2‑218 (MQ=255)                                                                                                 ggCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATAttt                                                                                                                               <  1:266301/219‑1 (MQ=255)                                                                                                 ggCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTg                                                                                                                           <  1:259137/223‑1 (MQ=255)                                                                                                 ggCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAtctttt                                                                                                >  1:226588/2‑251 (MQ=255)                                                                                                  gCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTc                                                                                                                                                                                                <  1:112598/154‑1 (MQ=255)                                                                                                  gCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAtctttt                                                                                                >  1:321199/1‑250 (MQ=255)                                                                                                      taccTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATcttttctttt                                                                                           >  1:391479/3‑251 (MQ=255)                                                                                                           aCTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTtttgtttg                                                                                      >  1:230276/1‑251 (MQ=255)                                                                                                              ttcttcaCAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAAc                                                                                   <  1:587261/245‑1 (MQ=255)                                                                                                                      agatagatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTggg                                                                           >  1:398006/1‑251 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTg                                                                                                                                                                                 <  1:182938/144‑1 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAg                                                                                                                                                                   <  1:273481/158‑1 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAAt                                                                                                              <  1:301991/211‑1 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTg                                                                             >  1:748234/1‑244 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCa                                                                      >  1:235684/1‑251 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCa                                                                      >  1:237979/1‑251 (MQ=255)                                                                                                                           gatTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGATGACAACAGAATCAACATTAACGGTATCAGGCGTCAAAGGCCGACCTTCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAgg                                                                                                                                                                  <  1:643571/159‑1 (MQ=255)                                                                                                                            atTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCAc                                                                     <  1:726530/251‑1 (MQ=255)                                                                                                                            atTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCAc                                                                     <  1:419808/251‑1 (MQ=255)                                                                                                                            atTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCAc                                                                     <  1:379363/251‑1 (MQ=255)                                                                                                                               aGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCtt                                                                  >  1:809596/1‑251 (MQ=255)                                                                                                                                gTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTc                                                                                                                                                                                                <  1:322637/124‑1 (MQ=255)                                                                                                                                gTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAtcttttcttt                                                                                            >  1:790791/1‑224 (MQ=255)                                                                                                                                       gaaaatggctcttcaGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCag                                                           <  1:252177/236‑1 (MQ=255)                                                                                                                                         aaatggctcttcaGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCagaga                                                        <  1:91018/239‑1 (MQ=255)                                                                                                                                            tggctcttcaGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGaa                                                     >  1:442610/10‑251 (MQ=255)                                                                                                                                               ctcttcaGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAGTCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAAtt                                                  >  1:809690/7‑251 (MQ=255)                                                                                                                                                 cttcaGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAg                                                                                                                                           >  1:551428/5‑160 (MQ=255)                                                                                                                                                 cttcaGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAAtttt                                                >  1:312855/5‑251 (MQ=255)                                                                                                                                                       ttgttTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGc                                            <  1:585934/249‑1 (MQ=255)                                                                                                                                                         gttTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTa                                         >  1:331450/1‑250 (MQ=255)                                                                                                                                                         gttTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGCTGCACCTTTATCCAGAGAGAAATTTTGTGCGTa                                         >  1:669579/1‑250 (MQ=255)                                                                                                                                                                  tatCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCGCCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAAt                               >  1:232526/3‑251 (MQ=255)                                                                                                                                                                     cTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATgag                            >  1:297018/1‑251 (MQ=255)                                                                                                                                                                          atgcTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACAt                       <  1:861191/248‑1 (MQ=255)                                                                                                                                                                                gtgacaacaGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGctc                  <  1:398083/247‑3 (MQ=255)                                                                                                                                                                                         gAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCAt                                    >  1:261636/1‑223 (MQ=255)                                                                                                                                                                                          aaTCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGc        <  1:551242/250‑1 (MQ=255)                                                                                                                                                                                           aTCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGcac      <  1:12346/251‑1 (MQ=255)                                                                                                                                                                                           aTCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGCGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAAc                                                                                   >  1:899541/1‑174 (MQ=255)                                                                                                                                                                                              aaCATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGc   <  1:98374/251‑1 (MQ=255)                                                                                                                                                                                               aCATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCTCCGAGAACTGCACAGCa  >  1:300522/1‑251 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                             TTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGATCTTTTCTTTTGTTTGAACCAACTGGGTTGCACCTTTATCCAGAGAGAAATTTTGTGCGTACGCATTTAATGAGAACATTGCCCCGAGAACTGCACAGCA  >  1/859156‑859596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑