Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,252	A→G	intergenic (‑9595/‑62966)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,252	0	A	G	100.0%	115.7 / NA	41	intergenic (‑9595/‑62966)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

TAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAT  >  1/859005‑859497                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAGCACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                                                                                    <  1:653579/251‑1 (MQ=255) tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                                                                                    <  1:894327/251‑1 (MQ=255) tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                                                                                    <  1:340674/251‑1 (MQ=255)      cccATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                                                                                                                                                                                                                               <  1:781966/251‑4 (MQ=255)         atcgCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTAc                                                                                                                                                                                                                                            <  1:244491/248‑1 (MQ=255)                   aaaGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACtttcttcac                                                                                                                                                                                                                                   <  1:216054/250‑7 (MQ=255)                       tttcttatttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACagat                                                                                                                                                                                                                               <  1:176042/246‑1 (MQ=255)                             ttttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAg                                                                                                                                                                                                                        <  1:837636/250‑1 (MQ=255)                                taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt                                                                                                                                                                                                                     <  1:862888/251‑1 (MQ=255)                                taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt                                                                                                                                                                                                                     <  1:795982/251‑1 (MQ=255)                                   ctctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTg                                                                                                                                                                                                                  >  1:251446/1‑251 (MQ=255)                                     ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                                                                                >  1:735964/1‑250 (MQ=255)                                     ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                                                                                >  1:742506/1‑250 (MQ=255)                                     ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                                                                                >  1:591569/1‑250 (MQ=255)                                       acaacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTggaaa                                                                                                                                                                                                              <  1:567193/251‑4 (MQ=255)                                        caacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                                                                                                                                                                                                                               >  1:546111/1‑214 (MQ=255)                                                                                                                                                        ttCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTc                                                                                             <  1:818469/251‑1 (MQ=255)                                                                                                                                                         tCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGtt                                                                                              >  1:80305/1‑249 (MQ=255)                                                                                                                                                             tAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTAc                                                                                          <  1:726356/249‑1 (MQ=255)                                                                                                                                                                             tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTc                                                                                                  >  1:556071/1‑225 (MQ=255)                                                                                                                                                                             tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGt                                                                                               >  1:395681/1‑228 (MQ=255)                                                                                                                                                                                   aGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTg                                                                              <  1:676765/239‑1 (MQ=255)                                                                                                                                                                                     cTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATaaa                                                                 <  1:703990/250‑1 (MQ=255)                                                                                                                                                                                        ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                                             <  1:597552/251‑1 (MQ=255)                                                                                                                                                                                        ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                                             <  1:605228/251‑1 (MQ=255)                                                                                                                                                                                                  tctacGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTa                                                   >  1:144644/5‑251 (MQ=255)                                                                                                                                                                                                       gAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                                             >  1:244589/1‑236 (MQ=255)                                                                                                                                                                                                                             tgaACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTg                        <  1:75060/250‑1 (MQ=255)                                                                                                                                                                                                                                         accttCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTtcctc                                                                                   >  1:224996/5‑180 (MQ=255)                                                                                                                                                                                                                                              cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTg                                                                                                >  1:455650/1‑162 (MQ=255)                                                                                                                                                                                                                                              cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTAc                                                                                          <  1:519009/168‑1 (MQ=255)                                                                                                                                                                                                                                              cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg       >  1:682816/1‑251 (MQ=255)                                                                                                                                                                                                                                              cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg       >  1:639839/1‑251 (MQ=255)                                                                                                                                                                                                                                              cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg       >  1:554590/1‑251 (MQ=255)                                                                                                                                                                                                                                              cAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAgtg       >  1:873240/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATaaa                                                                 <  1:337503/190‑1 (MQ=255)                                                                                                                                                                                                                                                   aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAAt           >  1:673619/1‑242 (MQ=255)                                                                                                                                                                                                                                                   aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt  >  1:641929/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt  >  1:737393/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt  >  1:580437/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aCATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAt  >  1:368532/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTGAAGAGACGAAAATAGTGTCGAT  >  1/859005‑859497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑