Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,231	G→A	intergenic (‑9574/‑62987)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,231	0	G	A	100.0%	107.4 / NA	39	intergenic (‑9574/‑62987)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

ACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTG  >  1/858992‑859475                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     aCGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttacctt                                                                                                                                                                                                                                            >  1:766454/1‑245 (MQ=255)  tattccgttctttAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttaccttca                                                                                                                                                                                                                                          >  1:175643/12‑244 (MQ=255)       cTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTa                                                                                                                                                                                                                                      >  1:548782/1‑250 (MQ=255)         ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                                                                                                                                                                                                                   <  1:17735/251‑1 (MQ=255)         ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                                                                                                                                                                                                                   <  1:769744/251‑1 (MQ=255)           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                                                                 >  1:159963/1‑250 (MQ=255)           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                                                                 >  1:237683/1‑250 (MQ=255)           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                                                                 >  1:878691/1‑250 (MQ=255)           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                                                                 >  1:806362/1‑250 (MQ=255)            gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                                                                                                                                                                                                                >  1:117591/1‑249 (MQ=255)            gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                                                                                                                                                                                                                >  1:226130/1‑249 (MQ=255)              tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAGCACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                                                              <  1:653579/251‑1 (MQ=255)              tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                                                              <  1:894327/251‑1 (MQ=255)              tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                                                              <  1:340674/251‑1 (MQ=255)                   cccATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                                                                                                                                                                                                         <  1:781966/251‑4 (MQ=255)                      atcgCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTAc                                                                                                                                                                                                                      <  1:244491/248‑1 (MQ=255)                                aaaGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACtttcttcac                                                                                                                                                                                                             <  1:216054/250‑7 (MQ=255)                                    tttcttatttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACagat                                                                                                                                                                                                         <  1:176042/246‑1 (MQ=255)                                          ttttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAg                                                                                                                                                                                                  <  1:837636/250‑1 (MQ=255)                                             taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt                                                                                                                                                                                               <  1:795982/251‑1 (MQ=255)                                             taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt                                                                                                                                                                                               <  1:862888/251‑1 (MQ=255)                                                ctctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTg                                                                                                                                                                                            >  1:251446/1‑251 (MQ=255)                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                                                          >  1:591569/1‑250 (MQ=255)                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                                                          >  1:742506/1‑250 (MQ=255)                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                                                          >  1:735964/1‑250 (MQ=255)                                                    acaacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTggaaa                                                                                                                                                                                        <  1:567193/251‑4 (MQ=255)                                                     caacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                                                                                                                                                                                                         >  1:546111/1‑214 (MQ=255)                                                                                                                                                                     ttCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTc                                                                       <  1:818469/251‑1 (MQ=255)                                                                                                                                                                      tCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGtt                                                                        >  1:80305/1‑249 (MQ=255)                                                                                                                                                                          tAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTAc                                                                    <  1:726356/249‑1 (MQ=255)                                                                                                                                                                                          tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTc                                                                            >  1:556071/1‑225 (MQ=255)                                                                                                                                                                                          tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGt                                                                         >  1:395681/1‑228 (MQ=255)                                                                                                                                                                                                aGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTg                                                        <  1:676765/239‑1 (MQ=255)                                                                                                                                                                                                  cTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATaaa                                           <  1:703990/250‑1 (MQ=255)                                                                                                                                                                                                     ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                       <  1:597552/251‑1 (MQ=255)                                                                                                                                                                                                     ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                       <  1:605228/251‑1 (MQ=255)                                                                                                                                                                                                               tctacGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTa                             >  1:144644/5‑251 (MQ=255)                                                                                                                                                                                                                    gAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg                                       >  1:244589/1‑236 (MQ=255)                                                                                                                                                                                                                                          tgaACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTg  <  1:75060/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTGTTGTCGTCTAAAATTTCATAGTCGGCAATATTTGGTG  >  1/858992‑859475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑