Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,201	G→A	intergenic (‑9544/‑63017)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,201	0	G	A	100.0%	116.9 / NA	44	intergenic (‑9544/‑63017)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/25);  total (19/25)

AACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTG  >  1/858954‑859438                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aaCCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGa                                                                                                                                                                                                                                            <  1:647886/251‑1 (MQ=255)           aCCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGc                                                                                                                                                                                                                                  <  1:276557/251‑1 (MQ=255)                  aCTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttcttc                                                                                                                                                                                                                           <  1:481702/251‑1 (MQ=255)                   cTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttctt                                                                                                                                                                                                                            >  1:494294/1‑249 (MQ=255)                     aaTGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTg                                                                                                                                                                                                                        <  1:686430/251‑1 (MQ=255)                                       aCGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttacctt                                                                                                                                                                                                       >  1:766454/1‑245 (MQ=255)                                        tattccgttctttAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttaccttca                                                                                                                                                                                                     >  1:175643/12‑244 (MQ=255)                                             cTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTa                                                                                                                                                                                                 >  1:548782/1‑250 (MQ=255)                                               ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                                                                                                                                                                              <  1:769744/251‑1 (MQ=255)                                               ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                                                                                                                                                                              <  1:17735/251‑1 (MQ=255)                                                 aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGa                                                                                                                                                                                                                                            >  1:391127/1‑203 (MQ=255)                                                 aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTgtg                                                                                                                                                                                                                      <  1:40864/225‑3 (MQ=255)                                                 aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                            >  1:806362/1‑250 (MQ=255)                                                 aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                            >  1:237683/1‑250 (MQ=255)                                                 aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                            >  1:878691/1‑250 (MQ=255)                                                 aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                                                                                                                                                                            >  1:159963/1‑250 (MQ=255)                                                  gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                                                                                                                                                                           >  1:226130/1‑249 (MQ=255)                                                  gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                                                                                                                                                                           >  1:117591/1‑249 (MQ=255)                                                    tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAGCACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                         <  1:653579/251‑1 (MQ=255)                                                    tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                         <  1:894327/251‑1 (MQ=255)                                                    tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                                                                                                                                                                         <  1:340674/251‑1 (MQ=255)                                                         cccATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                                                                                                                                                                    <  1:781966/251‑4 (MQ=255)                                                            atcgCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTAc                                                                                                                                                                                 <  1:244491/248‑1 (MQ=255)                                                                      aaaGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACtttcttcac                                                                                                                                                                        <  1:216054/250‑7 (MQ=255)                                                                          tttcttatttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACagat                                                                                                                                                                    <  1:176042/246‑1 (MQ=255)                                                                                ttttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAg                                                                                                                                                             <  1:837636/250‑1 (MQ=255)                                                                                   taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt                                                                                                                                                          <  1:862888/251‑1 (MQ=255)                                                                                   taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt                                                                                                                                                          <  1:795982/251‑1 (MQ=255)                                                                                      ctctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTg                                                                                                                                                       >  1:251446/1‑251 (MQ=255)                                                                                        ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttcttc                                                                                                                                                                                                                           <  1:361749/181‑1 (MQ=255)                                                                                        ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                     >  1:742506/1‑250 (MQ=255)                                                                                        ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                     >  1:735964/1‑250 (MQ=255)                                                                                        ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga                                                                                                                                                     >  1:591569/1‑250 (MQ=255)                                                                                          acaacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTggaaa                                                                                                                                                   <  1:567193/251‑4 (MQ=255)                                                                                           caacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                                                                                                                                                                    >  1:546111/1‑214 (MQ=255)                                                                                                                                                                                                           ttCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTc                                  <  1:818469/251‑1 (MQ=255)                                                                                                                                                                                                            tCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGtt                                   >  1:80305/1‑249 (MQ=255)                                                                                                                                                                                                                tAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTAc                               <  1:726356/249‑1 (MQ=255)                                                                                                                                                                                                                                tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTc                                       >  1:556071/1‑225 (MQ=255)                                                                                                                                                                                                                                tCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGt                                    >  1:395681/1‑228 (MQ=255)                                                                                                                                                                                                                                      aGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTg                   <  1:676765/239‑1 (MQ=255)                                                                                                                                                                                                                                        cTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATaaa      <  1:703990/250‑1 (MQ=255)                                                                                                                                                                                                                                           ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg  <  1:605228/251‑1 (MQ=255)                                                                                                                                                                                                                                           ctcCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTGGAAAATGGCTCTTCAGTTGTTTATTCGTATCTGCAATGCTGGTGACAACAGAATCAACATTAACGGTATCAGACGTCAAAGGCCGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGgtg  <  1:597552/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAAACGGCTTTTAAGTTGTTTATTCGTGTCTGCAATACTGGTCACAACAGAATCAACATTAACAGTATCGGATGTTAAGGGACGACCATCTGCTCCGAAAGCAGTAACTTCTGTTCTACCTTCCTCCTTTGCGTAGATAATAAAGGTG  >  1/858954‑859438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑