Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	859,075	A→G	intergenic (‑9418/‑63143)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	859,075	0	A	G	100.0%	212.4 / NA	77	intergenic (‑9418/‑63143)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/37);  total (40/37)

AGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAATAAAGGCAGAAATACCTTTCGCATCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAA  >  1/858832‑859293                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           aaaaaTGTTCGGTTCGGCCAGAACCTTACCATTACTTTGTTAATCTAAGGCATTAATAAACGCAAAAATACCTTTTGCGTCTAAACGTAATACCCCTTAATTTCCATTGAATCCCCCTCCAAAACCGAAGTTACCTCCGACT‑ATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAat                                                                                                                                                                                                                    <  1:870228/249‑1 (MQ=255)                ggCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCaa                                                                                                                                                                                                      >  1:520483/1‑251 (MQ=255)                ggCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCaa                                                                                                                                                                                                      >  1:612325/1‑251 (MQ=255)                 gCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCg                                                                                                                                                                                                             >  1:517859/1‑243 (MQ=255)                      aaccttACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAacac                                                                                                                                                                                                <  1:764481/247‑1 (MQ=255)                      aaccttACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAacac                                                                                                                                                                                                <  1:591270/247‑1 (MQ=255)                           tACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCAt                                                                                                                                                                                             >  1:47155/1‑249 (MQ=255)                           tACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCg                                                                                                                                                                                           >  1:246843/1‑251 (MQ=255)                           tACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCg                                                                                                                                                                                           >  1:24880/1‑251 (MQ=255)                                   cTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATggta                                                                                                                                                                                   <  1:751663/251‑4 (MQ=255)                                      tGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAacac                                                                                                                                                                                                >  1:683427/1‑235 (MQ=255)                                             cTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAg                                                                                                                                                                         >  1:653987/1‑251 (MQ=255)                                                     ttAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGaacggcaa                                                                                                                                                                 >  1:272800/1‑245 (MQ=255)                                                       aataaACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTc                                                                                                                                                                             <  1:66897/237‑1 (MQ=255)                                                        ataaACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAagc                                                                                                                                                              <  1:142977/251‑2 (MQ=255)                                                               cAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAacac                                                                                                                                                                                                >  1:897524/1‑210 (MQ=255)                                                                 gAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAagcatcttccc                                                                                                                                                      >  1:628113/1‑241 (MQ=255)                                                                  aaaTACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAagcatcttcccc                                                                                                                                                     <  1:527983/250‑11 (MQ=255)                                                                     tACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATgg                                                                                                                                                                                     >  1:802641/1‑214 (MQ=255)                                                                       ccTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAAcaac                                                                                                                                                                                                  >  1:412657/1‑197 (MQ=255)                                                                               gtCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAAca                                                                                                                                                                                                    <  1:841814/189‑2 (MQ=255)                                                                                     aCGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTg                                                                                                                                                                                                               >  1:254779/1‑173 (MQ=255)                                                                                     aCGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAAcaac                                                                                                                                                                                                  >  1:259762/1‑183 (MQ=255)                                                                                     aCGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAAc                                                                                                                                   >  1:341443/1‑249 (MQ=255)                                                                                      cGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGTCTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCtt                                                                                                                                >  1:92207/1‑251 (MQ=255)                                                                                       gTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGaacggcaa                                                                                                                                                                 <  1:41348/217‑7 (MQ=255)                                                                                           tACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTcgac                                                                                                                           <  1:227691/251‑4 (MQ=255)                                                                                             cacCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGAccg                                                                                                                         >  1:322667/1‑250 (MQ=255)                                                                                                   gATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGAccgtttc                                                                                                                     >  1:808615/1‑244 (MQ=255)                                                                                                     tttCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTaa                                                                                                                 <  1:430707/251‑1 (MQ=255)                                                                                                        ccATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGc                                                                                                              <  1:225218/251‑1 (MQ=255)                                                                                                          aTTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCtt                                                                                                            <  1:412058/251‑1 (MQ=255)                                                                                                          aTTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATAGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAAcaac                                                                                                                                                                                                  >  1:363553/1‑162 (MQ=255)                                                                                                                      tCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTc                                                                                                <  1:82274/251‑1 (MQ=255)                                                                                                                           aaCCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGa                                                                                           <  1:647886/251‑1 (MQ=255)                                                                                                                                     aCCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGc                                                                                 <  1:276557/251‑1 (MQ=255)                                                                                                                                        tCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCg                                                                                                                                                                                                             >  1:843136/1‑124 (MQ=255)                                                                                                                                            aCTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttcttc                                                                          <  1:481702/251‑1 (MQ=255)                                                                                                                                             cTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATgg                                                                                                                                                                                     >  1:596387/1‑142 (MQ=255)                                                                                                                                             cTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTc                                                                                                                                         <  1:804591/187‑1 (MQ=255)                                                                                                                                             cTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttctt                                                                           >  1:494294/1‑249 (MQ=255)                                                                                                                                               aaTGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTg                                                                       <  1:686430/251‑1 (MQ=255)                                                                                                                                                aTGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGc                                                                                                              <  1:305182/211‑1 (MQ=255)                                                                                                                                                                 aCGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttacctt                                                      >  1:766454/1‑245 (MQ=255)                                                                                                                                                                  tattccgttctttAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCtttaccttca                                                    >  1:175643/12‑244 (MQ=255)                                                                                                                                                                       cTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTa                                                >  1:548782/1‑250 (MQ=255)                                                                                                                                                                         ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAagcat                                                                                                                                                            >  1:121394/1‑137 (MQ=255)                                                                                                                                                                         ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                             <  1:17735/251‑1 (MQ=255)                                                                                                                                                                         ccAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACAt                                             <  1:769744/251‑1 (MQ=255)                                                                                                                                                                          cAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGaa                                                                                                                                                                       <  1:604360/128‑1 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGaa                                                                                                                                                                       >  1:83425/1‑127 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTcgac                                                                                                                           <  1:175589/171‑4 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGa                                                                                           >  1:391127/1‑203 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTgtg                                                                     <  1:40864/225‑3 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:237683/1‑250 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:159963/1‑250 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:806362/1‑250 (MQ=255)                                                                                                                                                                           aGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATag                                           >  1:878691/1‑250 (MQ=255)                                                                                                                                                                            gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                          >  1:117591/1‑249 (MQ=255)                                                                                                                                                                            gTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATagg                                          >  1:226130/1‑249 (MQ=255)                                                                                                                                                                              tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAGCACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                        <  1:653579/251‑1 (MQ=255)                                                                                                                                                                              tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                        <  1:340674/251‑1 (MQ=255)                                                                                                                                                                              tAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGcc                                        <  1:894327/251‑1 (MQ=255)                                                                                                                                                                                   cccATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                   <  1:781966/251‑4 (MQ=255)                                                                                                                                                                                      atcgCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTAc                                <  1:244491/248‑1 (MQ=255)                                                                                                                                                                                                aaaGTTTCTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACtttcttcac                       <  1:216054/250‑7 (MQ=255)                                                                                                                                                                                                    tttcttatttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACagat                   <  1:176042/246‑1 (MQ=255)                                                                                                                                                                                                          ttttaCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAg            <  1:837636/250‑1 (MQ=255)                                                                                                                                                                                                             taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt         <  1:862888/251‑1 (MQ=255)                                                                                                                                                                                                             taCCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTAt         <  1:795982/251‑1 (MQ=255)                                                                                                                                                                                                                ctctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTg      >  1:251446/1‑251 (MQ=255)                                                                                                                                                                                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTttcttc                                                                          <  1:361749/181‑1 (MQ=255)                                                                                                                                                                                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga    >  1:735964/1‑250 (MQ=255)                                                                                                                                                                                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga    >  1:742506/1‑250 (MQ=255)                                                                                                                                                                                                                  ctACAACAGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTgga    >  1:591569/1‑250 (MQ=255)                                                                                                                                                                                                                    acaacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCTTACCTACTTTCTTCACAGATAGATTAGTATTTggaaa  <  1:567193/251‑4 (MQ=255)                                                                                                                                                                                                                     caacaGAAAGCTTCACATTAATTTGGGTTGTGTCTGCAATATTTGCGTTATCAACAACACCATCGTAATGGTATTTGTCTAAGAACGGCAAAGCATCTTCCCCTAACTTTGTTTCAGTAACCTTTCGACCGTTTCCTAAAGCTTCTCCTACAATTCTACGAACTTCATCGCTTTCTTCTTGTGAACGAGCTTTACCTTCAATTACATAGGCCttacc                                   >  1:546111/1‑214 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           AGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAATAAAGGCAGAAATACCTTTCGCATCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGCTTCACATTAATTTGGGTTGTATCGGCAATATTTGCGTTATCAACGACACCATCGTAATGATATTTGTCTAAGAATGGTAAAGAGTCTTCTCCTAATTTTGTTTCAGTAACCTTTCTACCACTTCCTAAAGCTTCTCCTACAATTCTGCGAACCTCATCGCTTTCTTCTTGAGAACGGGCTTTTCCCTCAATTACATAAGCCTTGCCTACTTTTTTTACAGATAGATTAGTATTTGGGAA  >  1/858832‑859293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑