Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,823	G→T	intergenic (‑9166/‑63395)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,823	0	G	T	100.0%	205.7 / NA	76	intergenic (‑9166/‑63395)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/35);  total (41/35)

ATTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑TTCCATCAAAGTTATAGTTACCTGCAATAGTGCTTACTGTTTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTAGCCGCAACAGCGAGTTTTATCCCAAACTCCTTATAGATAATTGTTGGTGAACCATCTCTATCCCGCTGTGCAAATGGGATTTCTCCCCCCACTAAGATATCGGCAGTTTCACCGGATAACATAGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAATAAAGGCAGAAATACCTTTCGCATCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGC  >  1/858588‑859054                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGACTTCC‑TCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                                                            <  1:699856/247‑1 (MQ=255) actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                                                            <  1:42944/247‑1 (MQ=255) actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATGTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                                                            <  1:619916/247‑1 (MQ=255)      gCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATgttcgg                                                                                                                                                                                                                     >  1:503844/1‑251 (MQ=255)              ggTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGAt                                                                                                                                                                                                                                      >  1:385210/1‑226 (MQ=255)               gTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAg                                                                                                                                                                                                            >  1:132618/1‑251 (MQ=255)                    aaaGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAAc                                                                                                                                                                                                         >  1:858954/1‑249 (MQ=255)                            gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTAc                                                                                                                                                                                                    <  1:610723/246‑1 (MQ=255)                            gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCAtt                                                                                                                                                                                                <  1:54873/250‑1 (MQ=255)                             aaTATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTAc                                                                                                                                                                                              <  1:183680/251‑1 (MQ=255)                                atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACttt                                                                                                                                                                                           >  1:203713/1‑251 (MQ=255)                                atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACttt                                                                                                                                                                                           >  1:33771/1‑251 (MQ=255)                                   cccGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGt                                                                                                                                                                                        <  1:522610/251‑1 (MQ=255)                                                   gTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                                                            <  1:591550/200‑1 (MQ=255)                                                    ttATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaat                                                                                                                                                                        <  1:2316/251‑1 (MQ=255)                                                           taccagctatggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCa                                                                                                                                                                 >  1:716543/12‑251 (MQ=255)                                                              cagctatggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATaaacgcggaa                                                                                                                                                              >  1:419890/9‑244 (MQ=255)                                                                gctatggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGtt                                                                                                                                                                                       >  1:791753/7‑224 (MQ=255)                                                                   atggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                                                            >  1:580253/4‑184 (MQ=255)                                                                    tggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCt                                                                                                                                                        <  1:685726/249‑1 (MQ=255)                                                                     ggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATgt                                                                                                                                                                                                                         <  1:582482/184‑1 (MQ=255)                                                                     ggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAAc                                                                                                                                                                                                         <  1:269539/200‑1 (MQ=255)                                                                                  tGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACg                                                                                                                                          <  1:514622/251‑1 (MQ=255)                                                                                  tGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACg                                                                                                                                          <  1:869532/251‑1 (MQ=255)                                                                                   gATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaat                                                                                                                                                                        >  1:303161/1‑220 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTAc                                                                                                                                                                                              <  1:637786/197‑1 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTaaggc                                                                                                                                                                              <  1:17872/213‑4 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaa                                                                                                                                                                         <  1:398208/218‑1 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATaaa                                                                                                                                                                     <  1:348614/222‑1 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTa                                                                                                                                        >  1:592527/1‑251 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTa                                                                                                                                        >  1:184271/1‑251 (MQ=255)                                                                                    aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTa                                                                                                                                        >  1:300619/1‑251 (MQ=255)                                                                                          ggCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGTAACAGCGAGCTTTATCCCAAACTCCTTATATATACTTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGAGATCGGCAGTTTCACCTGACAACATAGAAATGTTCGGTTCGGCCAGTACCTTACCATTACTTTGGTTATCTAAGGCATTAATaaacgc                                                                                                                                                                  >  1:510225/1‑216 (MQ=255)                                                                                                   aaTACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGAttt                                                                                                                          <  1:735236/250‑1 (MQ=255)                                                                                                           tattTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAAt                                                                                                                 <  1:351871/251‑1 (MQ=255)                                                                                                            attTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATc                                                                                                                <  1:2014/251‑1 (MQ=255)                                                                                                                  ttGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTc                                                                                                          <  1:532332/251‑1 (MQ=255)                                                                                                                                  aCAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATac                                                                                                                                    >  1:688489/1‑209 (MQ=255)                                                                                                                                         gctttATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGa                                                                                     >  1:595627/3‑249 (MQ=255)                                                                                                                                              aTCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATgg                                                                              <  1:421541/251‑1 (MQ=255)                                                                                                                                                      cTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGaaa                                                                                                                                                             <  1:249823/164‑1 (MQ=255)                                                                                                                                                      cTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTa                                                                       >  1:627781/1‑250 (MQ=255)                                                                                                                                                        ccTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATac                                                                                                                                    >  1:134773/1‑187 (MQ=255)                                                                                                                                                          ttATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGc                                                                  <  1:420350/251‑1 (MQ=255)                                                                                                                                                            atataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGt                                                                                                                                         >  1:754848/5‑178 (MQ=255)                                                                                                                                                            atataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATgg                                                                              >  1:753350/5‑237 (MQ=255)                                                                                                                                                            atataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCaa                                                                >  1:13981/5‑251 (MQ=255)                                                                                                                                                              ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAAc                                                                                                    >  1:839881/3‑213 (MQ=255)                                                                                                                                                              ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGc                                                                  >  1:226137/3‑247 (MQ=255)                                                                                                                                                              ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACg                                                              >  1:760940/3‑251 (MQ=255)                                                                                                                                                              ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACg                                                              >  1:372500/3‑251 (MQ=255)                                                                                                                                                              ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACg                                                              >  1:464950/3‑251 (MQ=255)                                                                                                                                                                   aTTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTga                                                           >  1:568677/1‑248 (MQ=255)                                                                                                                                                                              aCCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAAtac                                              <  1:195924/251‑3 (MQ=255)                                                                                                                                                                                 aTCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCa                                           >  1:403054/1‑251 (MQ=255)                                                                                                                                                                                       atctcgttgtgCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCAtcgc                                       >  1:502991/8‑246 (MQ=255)                                                                                                                                                                                            gttgtgCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGaa                                >  1:501298/3‑251 (MQ=255)                                                                                                                                                                                               gtgCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTAc                                                                                                                                                                                              <  1:325592/90‑1 (MQ=255)                                                                                                                                                                                               gtgCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGtt                                                   >  1:644635/1‑229 (MQ=255)                                                                                                                                                                                               gtgCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAAtac                                              >  1:474023/1‑232 (MQ=255)                                                                                                                                                                                                    aaTGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGtttctt                        >  1:838673/1‑248 (MQ=255)                                                                                                                                                                                                      tGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTcttat                      <  1:687412/251‑1 (MQ=255)                                                                                                                                                                                                        ggATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTcttattt                    >  1:415451/1‑251 (MQ=255)                                                                                                                                                                                                           tttcacctcccACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTAc                  >  1:558930/9‑250 (MQ=255)                                                                                                                                                                                                           tttcacctcccACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAACACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTAcc                 <  1:630824/243‑1 (MQ=255)                                                                                                                                                                                                                   cccACTAGGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTacaa          >  1:319096/1‑250 (MQ=255)                                                                                                                                                                                                                   cccACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAAtac                                              <  1:701386/214‑3 (MQ=255)                                                                                                                                                                                                                   cccACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTacaac         <  1:624125/251‑1 (MQ=255)                                                                                                                                                                                                                     cACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATacac                                                                                                                                  >  1:391034/1‑128 (MQ=255)                                                                                                                                                                                                                     cACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAg       <  1:465496/251‑1 (MQ=255)                                                                                                                                                                                                                     cACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAg       >  1:448773/1‑251 (MQ=255)                                                                                                                                                                                                                      aCTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAAtac                                              <  1:541170/211‑3 (MQ=255)                                                                                                                                                                                                                       cTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCt                                                                                                                                                        >  1:316470/1‑104 (MQ=255)                                                                                                                                                                                                                           gATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAg   >  1:831077/1‑249 (MQ=255)                                                                                                                                                                                                                            atatCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCATCGCCTCAGAAAGTTTCTTATTTACCTCTACAACAGAAAGc  <  1:180434/249‑1 (MQ=255)                                                                                                                                                                                                                                    aGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGAc                                                                            <  1:859420/167‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        ATTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑TTCCATCAAAGTTATAGTTACCTGCAATAGTGCTTACTGTTTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTAGCCGCAACAGCGAGTTTTATCCCAAACTCCTTATAGATAATTGTTGGTGAACCATCTCTATCCCGCTGTGCAAATGGGATTTCTCCCCCCACTAAGATATCGGCAGTTTCACCGGATAACATAGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAATAAAGGCAGAAATACCTTTCGCATCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCATTGCCTCAGAAAGTTTTTTATTTACCTCTACAACAGAAAGC  >  1/858588‑859054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑