Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,772	C→T	intergenic (‑9115/‑63446)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,772	0	C	T	100.0%	183.8 / NA	66	intergenic (‑9115/‑63446)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/31);  total (35/31)

CTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACCTCAAATAAAGATTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑TTCCATCAAAGTTATAGTTACCTGCAATAGTGCTTACTGTTTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTAGCCGCAACAGCGAGTTTTATCCCAAACTCCTTATAGATAATTGTTGGTGAACCATCTCTATCCCGCTGTGCAAATGGGATTTCTCCCCCCACTAAGATATCGGCAGTTTCACCGGATAACATAGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAATAAAGGCAGAAATACCTTTCGCATCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCA  >  1/858536‑859013                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  cTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATg                                                                                                                                                                                                                                      <  1:686939/250‑1 (MQ=255)   gAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGa                                                                                                                                                                                                                                   >  1:298449/1‑251 (MQ=255)   gAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTA‑ATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGAt                                                                                                                                                                                                                                  >  1:610478/1‑251 (MQ=255)                   ataaAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTaa                                                                                                                                                                                                                   >  1:572700/1‑251 (MQ=255)                      aaaCTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGat                                                                                                                                                                                                                <  1:672845/251‑1 (MQ=255)                      aaaCTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGat                                                                                                                                                                                                                <  1:537119/251‑1 (MQ=255)                                       tacttCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCAcctg                                                                                                                                                                                               <  1:299047/247‑3 (MQ=255)                                        acttCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCAcctga                                                                                                                                                                                              >  1:458771/4‑248 (MQ=255)                                                   agactttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTA‑TTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGAtt                                                                                                                                                                                                                                 >  1:213319/5‑204 (MQ=255)                                                     actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGACTTCC‑TCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                   <  1:699856/247‑1 (MQ=255)                                                     actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                   <  1:42944/247‑1 (MQ=255)                                                     actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATGTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                   <  1:619916/247‑1 (MQ=255)                                                          gCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATgttcgg                                                                                                                                                                            >  1:503844/1‑251 (MQ=255)                                                                  ggTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGAt                                                                                                                                                                                             >  1:385210/1‑226 (MQ=255)                                                                   gTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAg                                                                                                                                                                   >  1:132618/1‑251 (MQ=255)                                                                        aaaGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAAc                                                                                                                                                                >  1:858954/1‑249 (MQ=255)                                                                                gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTAc                                                                                                                                                           <  1:610723/246‑1 (MQ=255)                                                                                gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCAtt                                                                                                                                                       <  1:54873/250‑1 (MQ=255)                                                                                 aaTATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTAc                                                                                                                                                     <  1:183680/251‑1 (MQ=255)                                                                                    atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACttt                                                                                                                                                  >  1:203713/1‑251 (MQ=255)                                                                                    atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACttt                                                                                                                                                  >  1:33771/1‑251 (MQ=255)                                                                                       cccGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGt                                                                                                                                               <  1:522610/251‑1 (MQ=255)                                                                                                       gTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                   <  1:591550/200‑1 (MQ=255)                                                                                                        ttATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaat                                                                                                                               <  1:2316/251‑1 (MQ=255)                                                                                                               taccagctatggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCa                                                                                                                        >  1:716543/12‑251 (MQ=255)                                                                                                                  cagctatggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATaaacgcggaa                                                                                                                     >  1:419890/9‑244 (MQ=255)                                                                                                                    gctatggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGtt                                                                                                                                              >  1:791753/7‑224 (MQ=255)                                                                                                                       atggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                                                                                                                                                   >  1:580253/4‑184 (MQ=255)                                                                                                                        tggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCt                                                                                                               <  1:685726/249‑1 (MQ=255)                                                                                                                         ggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATgt                                                                                                                                                                                <  1:582482/184‑1 (MQ=255)                                                                                                                         ggTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAAc                                                                                                                                                                <  1:269539/200‑1 (MQ=255)                                                                                                                               tACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGatat                                                                                                                                                                                                              >  1:781322/1‑149 (MQ=255)                                                                                                                                  tgattGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATg                                                                                                                                                                                                                                      <  1:489199/119‑1 (MQ=255)                                                                                                                                      tGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACg                                                                                                 <  1:514622/251‑1 (MQ=255)                                                                                                                                      tGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACg                                                                                                 <  1:869532/251‑1 (MQ=255)                                                                                                                                       gATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaat                                                                                                                               >  1:303161/1‑220 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTAc                                                                                                                                                     <  1:637786/197‑1 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTaaggc                                                                                                                                     <  1:17872/213‑4 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaa                                                                                                                                <  1:398208/218‑1 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATaaa                                                                                                                            <  1:348614/222‑1 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTa                                                                                               >  1:300619/1‑251 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTa                                                                                               >  1:184271/1‑251 (MQ=255)                                                                                                                                        aTCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTa                                                                                               >  1:592527/1‑251 (MQ=255)                                                                                                                                              ggCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGTAACAGCGAGCTTTATCCCAAACTCCTTATATATACTTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGAGATCGGCAGTTTCACCTGACAACATAGAAATGTTCGGTTCGGCCAGTACCTTACCATTACTTTGGTTATCTAAGGCATTAATaaacgc                                                                                                                         >  1:510225/1‑216 (MQ=255)                                                                                                                                                       aaTACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGAttt                                                                                 <  1:735236/250‑1 (MQ=255)                                                                                                                                                               tattTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAAt                                                                        <  1:351871/251‑1 (MQ=255)                                                                                                                                                                attTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATc                                                                       <  1:2014/251‑1 (MQ=255)                                                                                                                                                                      ttGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTc                                                                 <  1:532332/251‑1 (MQ=255)                                                                                                                                                                                      aCAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATac                                                                                           >  1:688489/1‑209 (MQ=255)                                                                                                                                                                                             gctttATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGa                                            >  1:595627/3‑249 (MQ=255)                                                                                                                                                                                                  aTCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATgg                                     <  1:421541/251‑1 (MQ=255)                                                                                                                                                                                                          cTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGaaa                                                                                                                    <  1:249823/164‑1 (MQ=255)                                                                                                                                                                                                          cTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTa                              >  1:627781/1‑250 (MQ=255)                                                                                                                                                                                                            ccTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATac                                                                                           >  1:134773/1‑187 (MQ=255)                                                                                                                                                                                                              ttATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGc                         <  1:420350/251‑1 (MQ=255)                                                                                                                                                                                                                atataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGt                                                                                                >  1:754848/5‑178 (MQ=255)                                                                                                                                                                                                                atataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATgg                                     >  1:753350/5‑237 (MQ=255)                                                                                                                                                                                                                atataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCaa                       >  1:13981/5‑251 (MQ=255)                                                                                                                                                                                                                  ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAAc                                                           >  1:839881/3‑213 (MQ=255)                                                                                                                                                                                                                  ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGc                         >  1:226137/3‑247 (MQ=255)                                                                                                                                                                                                                  ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACg                     >  1:372500/3‑251 (MQ=255)                                                                                                                                                                                                                  ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACg                     >  1:760940/3‑251 (MQ=255)                                                                                                                                                                                                                  ataTAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACg                     >  1:464950/3‑251 (MQ=255)                                                                                                                                                                                                                       aTTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTga                  >  1:568677/1‑248 (MQ=255)                                                                                                                                                                                                                                  aCCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAAtac     <  1:195924/251‑3 (MQ=255)                                                                                                                                                                                                                                     aTCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATTAATAAACGCAGAAATACCTTTTGCGTCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGACTCCAGTTAATACCCa  >  1:403054/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  CTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACCTCAAATAAAGATTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑TTCCATCAAAGTTATAGTTACCTGCAATAGTGCTTACTGTTTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTAGCCGCAACAGCGAGTTTTATCCCAAACTCCTTATAGATAATTGTTGGTGAACCATCTCTATCCCGCTGTGCAAATGGGATTTCTCCCCCCACTAAGATATCGGCAGTTTCACCGGATAACATAGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAATAAAGGCAGAAATACCTTTCGCATCTAAACGTAATACACCTTGATTTCCATTGAATCCACCTCCGAAACCGAAGTTACCTCCGACTAATGGACCACTACCGGCAACGTGGCTCCAGTTAATGCCCA  >  1/858536‑859013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑